Tags

Type your tag names separated by a space and hit enter

Multilocus sequence typing of Cryptococcus neoformans in non-HIV associated cryptococcosis in Nagasaki, Japan.
Med Mycol. 2013 Apr; 51(3):252-60.MM

Abstract

Cryptococcosis is primarily caused by two Cryptococcus species, i.e., Cryptococcus neoformans and C. gattii. Both include several genetically diverse subgroups that can be differentiated using various molecular strain typing methods. Since little is known about the molecular epidemiology of the C. neoformans/C. gattii species complex in Japan, we conducted a molecular epidemiological analysis of 35 C. neoformans isolates from non-HIV patients in Nagasaki, Japan and 10 environmental isolates from Thailand. All were analyzed using URA5-restriction fragment length polymorphism (RFLP) and multilocus sequence typing (MLST). Combined sequence data for all isolates were evaluated with the neighbor-joining method. All were found to be serotype A and mating type MATα. Thirty-two of the 35 clinical isolates molecular type VNI, while the three remaining isolates were VNII as determined through the URA5-RFLP method. Thirty-one of the VNI isolates were identified as MLST sequence type (ST) 5, the remaining one was ST 32 and the three VNII isolates were found to be ST 43. All the environmental isolates were identified as molecular type VNI (four MLST ST 5 and six ST 4). Our study shows that C. neoformans isolates in Nagasaki are genetically homogeneous, with most of the isolates being ST 5.

Links

Publisher Full Text

Authors+Show Affiliations

Mihara T
Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Izumikawa K
No affiliation info available
Kakeya H
No affiliation info available
Ngamskulrungroj P
No affiliation info available
Umeyama T
No affiliation info available
Takazono T
No affiliation info available
Tashiro M
No affiliation info available
Nakamura S
No affiliation info available
Imamura Y
No affiliation info available
Miyazaki T
No affiliation info available
Ohno H
No affiliation info available
Yamamoto Y
No affiliation info available
Yanagihara K
No affiliation info available
Miyzaki Y
No affiliation info available
Kohno S
No affiliation info available

MeSH

AdultAgedAged, 80 and overCluster AnalysisCryptococcosisCryptococcus neoformansEnvironmental MicrobiologyFemaleGenes, Mating Type, FungalGenotypeHumansJapanMaleMiddle AgedMolecular EpidemiologyMultilocus Sequence TypingMycological Typing TechniquesPolymorphism, Restriction Fragment LengthSerotypingThailandYoung Adult

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22901045

Citation

Mihara, Tomo, et al. "Multilocus Sequence Typing of Cryptococcus Neoformans in non-HIV Associated Cryptococcosis in Nagasaki, Japan." Medical Mycology, vol. 51, no. 3, 2013, pp. 252-60.
Mihara T, Izumikawa K, Kakeya H, et al. Multilocus sequence typing of Cryptococcus neoformans in non-HIV associated cryptococcosis in Nagasaki, Japan. Med Mycol. 2013;51(3):252-60.
Mihara, T., Izumikawa, K., Kakeya, H., Ngamskulrungroj, P., Umeyama, T., Takazono, T., Tashiro, M., Nakamura, S., Imamura, Y., Miyazaki, T., Ohno, H., Yamamoto, Y., Yanagihara, K., Miyzaki, Y., & Kohno, S. (2013). Multilocus sequence typing of Cryptococcus neoformans in non-HIV associated cryptococcosis in Nagasaki, Japan. Medical Mycology, 51(3), 252-60. https://doi.org/10.3109/13693786.2012.708883
Mihara T, et al. Multilocus Sequence Typing of Cryptococcus Neoformans in non-HIV Associated Cryptococcosis in Nagasaki, Japan. Med Mycol. 2013;51(3):252-60. PubMed PMID: 22901045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multilocus sequence typing of Cryptococcus neoformans in non-HIV associated cryptococcosis in Nagasaki, Japan. AU - Mihara,Tomo, AU - Izumikawa,Koichi, AU - Kakeya,Hiroshi, AU - Ngamskulrungroj,Popchai, AU - Umeyama,Takashi, AU - Takazono,Takahiro, AU - Tashiro,Masato, AU - Nakamura,Shigeki, AU - Imamura,Yoshifumi, AU - Miyazaki,Taiga, AU - Ohno,Hideaki, AU - Yamamoto,Yoshihiro, AU - Yanagihara,Katsunori, AU - Miyzaki,Yoshitsugu, AU - Kohno,Shigeru, Y1 - 2012/08/17/ PY - 2012/8/21/entrez PY - 2012/8/21/pubmed PY - 2013/12/16/medline SP - 252 EP - 60 JF - Medical mycology JO - Med Mycol VL - 51 IS - 3 N2 - Cryptococcosis is primarily caused by two Cryptococcus species, i.e., Cryptococcus neoformans and C. gattii. Both include several genetically diverse subgroups that can be differentiated using various molecular strain typing methods. Since little is known about the molecular epidemiology of the C. neoformans/C. gattii species complex in Japan, we conducted a molecular epidemiological analysis of 35 C. neoformans isolates from non-HIV patients in Nagasaki, Japan and 10 environmental isolates from Thailand. All were analyzed using URA5-restriction fragment length polymorphism (RFLP) and multilocus sequence typing (MLST). Combined sequence data for all isolates were evaluated with the neighbor-joining method. All were found to be serotype A and mating type MATα. Thirty-two of the 35 clinical isolates molecular type VNI, while the three remaining isolates were VNII as determined through the URA5-RFLP method. Thirty-one of the VNI isolates were identified as MLST sequence type (ST) 5, the remaining one was ST 32 and the three VNII isolates were found to be ST 43. All the environmental isolates were identified as molecular type VNI (four MLST ST 5 and six ST 4). Our study shows that C. neoformans isolates in Nagasaki are genetically homogeneous, with most of the isolates being ST 5. SN - 1460-2709 UR - https://www.unboundmedicine.com/medline/citation/22901045/Multilocus_sequence_typing_of_Cryptococcus_neoformans_in_non_HIV_associated_cryptococcosis_in_Nagasaki_Japan_ L2 - https://academic.oup.com/mmy/article-lookup/doi/10.3109/13693786.2012.708883 DB - PRIME DP - Unbound Medicine ER -