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A miRNA signature in leukocytes from sporadic amyotrophic lateral sclerosis.
Gene. 2012 Oct 15; 508(1):35-40.GENE

Abstract

Amyotrophic lateral sclerosis (ALS) is a progressive and seriously disabling adult-onset neurological disease. Accumulating evidence indicates that various miRNAs, expressed in a spatially and temporally controlled manner in the brain, play a key role in neuronal development. In addition, misregulation of microRNAs contributes to some mental disorders and neurodegeneration diseases. Here, we analyzed the expression profiles of 911 human miRNAs using microarray technology in leukocytes, the most readily available human tissue cells, obtained from 8 patients affected by sporadic amyotrophic lateral sclerosis (sALS) and 12 healthy controls. An independent group of 14 sALS patients and 14 controls was used for validation by TaqMan real-time polymerase chain reaction assay. We identified 8 miRNAs that were significantly up- or downregulated in sALS patients as compared to healthy controls. The significant variations in miRNAs profiles detected in leukocytes have been related to miRNAs predominantly expressed in the nervous system. One of these miRNAs, miR-338-3p, has previously been shown to be de-regulated in ALS brains. This study, for the first time, detected specific microRNAs disease-related changes at an earlier stage of sALS. We suggest that miRNAs profiles found in the peripheral blood leukocytes from sALS patients can be relevant to understand the pathogenesis of sALS and/or used as biomarkers of the disease.

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Authors+Show Affiliations

De Felice B
Department of Life Sciences, University of Naples II, Via Vivaldi 43, 81100 Caserta, Italy. bruna.defelice@unina2.it
Guida M
No affiliation info available
Guida M
No affiliation info available
Coppola C
No affiliation info available
De Mieri G
No affiliation info available
Cotrufo R
No affiliation info available

MeSH

AgedAmyotrophic Lateral SclerosisBiomarkersExcitatory Amino Acid Transporter 2FemaleGene Expression ProfilingGenetic Predisposition to DiseaseGlutamate Plasma Membrane Transport ProteinsHumansLeukocytesMaleMicroRNAsMiddle AgedOligonucleotide Array Sequence AnalysisReal-Time Polymerase Chain Reaction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22903028

Citation

De Felice, Bruna, et al. "A miRNA Signature in Leukocytes From Sporadic Amyotrophic Lateral Sclerosis." Gene, vol. 508, no. 1, 2012, pp. 35-40.
De Felice B, Guida M, Guida M, et al. A miRNA signature in leukocytes from sporadic amyotrophic lateral sclerosis. Gene. 2012;508(1):35-40.
De Felice, B., Guida, M., Guida, M., Coppola, C., De Mieri, G., & Cotrufo, R. (2012). A miRNA signature in leukocytes from sporadic amyotrophic lateral sclerosis. Gene, 508(1), 35-40. https://doi.org/10.1016/j.gene.2012.07.058
De Felice B, et al. A miRNA Signature in Leukocytes From Sporadic Amyotrophic Lateral Sclerosis. Gene. 2012 Oct 15;508(1):35-40. PubMed PMID: 22903028.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A miRNA signature in leukocytes from sporadic amyotrophic lateral sclerosis. AU - De Felice,Bruna, AU - Guida,Marco, AU - Guida,Maurizio, AU - Coppola,Cinzia, AU - De Mieri,Giovanna, AU - Cotrufo,Roberto, Y1 - 2012/08/08/ PY - 2012/04/13/received PY - 2012/07/19/revised PY - 2012/07/30/accepted PY - 2012/8/21/entrez PY - 2012/8/21/pubmed PY - 2012/11/9/medline SP - 35 EP - 40 JF - Gene JO - Gene VL - 508 IS - 1 N2 - Amyotrophic lateral sclerosis (ALS) is a progressive and seriously disabling adult-onset neurological disease. Accumulating evidence indicates that various miRNAs, expressed in a spatially and temporally controlled manner in the brain, play a key role in neuronal development. In addition, misregulation of microRNAs contributes to some mental disorders and neurodegeneration diseases. Here, we analyzed the expression profiles of 911 human miRNAs using microarray technology in leukocytes, the most readily available human tissue cells, obtained from 8 patients affected by sporadic amyotrophic lateral sclerosis (sALS) and 12 healthy controls. An independent group of 14 sALS patients and 14 controls was used for validation by TaqMan real-time polymerase chain reaction assay. We identified 8 miRNAs that were significantly up- or downregulated in sALS patients as compared to healthy controls. The significant variations in miRNAs profiles detected in leukocytes have been related to miRNAs predominantly expressed in the nervous system. One of these miRNAs, miR-338-3p, has previously been shown to be de-regulated in ALS brains. This study, for the first time, detected specific microRNAs disease-related changes at an earlier stage of sALS. We suggest that miRNAs profiles found in the peripheral blood leukocytes from sALS patients can be relevant to understand the pathogenesis of sALS and/or used as biomarkers of the disease. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/22903028/A_miRNA_signature_in_leukocytes_from_sporadic_amyotrophic_lateral_sclerosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(12)00909-2 DB - PRIME DP - Unbound Medicine ER -