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Improved method for isolation of lycopsamine from roots of comfrey (Symphytum officinale).
Nat Prod Commun 2012; 7(7):861-2NP

Abstract

An improved method for the isolation and purification of pyrrolizidine alkaloids from comfrey (Symphytum officinale L.) roots was developed, introducing very fast, selective and ion residue-free reduction of N-oxides followed by ion-exchange chromatography giving a non-aqueous solution of alkaloids, from which solvents can be easily removed. With this procedure the use of large volumes of organic solvents, very slow reduction of N-oxides and input of additional impurities was avoided. Lycopsamine, which proved to be the major alkaloid, was additionally purified by preparative layer chromatography (PLC) and high performance liquid chromatography (HPLC). The identity of the alkaloid was confirmed by (I)H NMR spectroscopy and mass spectrometry.

Authors+Show Affiliations

Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Slovenia. damjan.janes@ffa.uni-lj.siNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22908565

Citation

Janes, Damjan, et al. "Improved Method for Isolation of Lycopsamine From Roots of Comfrey (Symphytum Officinale)." Natural Product Communications, vol. 7, no. 7, 2012, pp. 861-2.
Janes D, Kalamar B, Kreft S. Improved method for isolation of lycopsamine from roots of comfrey (Symphytum officinale). Nat Prod Commun. 2012;7(7):861-2.
Janes, D., Kalamar, B., & Kreft, S. (2012). Improved method for isolation of lycopsamine from roots of comfrey (Symphytum officinale). Natural Product Communications, 7(7), pp. 861-2.
Janes D, Kalamar B, Kreft S. Improved Method for Isolation of Lycopsamine From Roots of Comfrey (Symphytum Officinale). Nat Prod Commun. 2012;7(7):861-2. PubMed PMID: 22908565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved method for isolation of lycopsamine from roots of comfrey (Symphytum officinale). AU - Janes,Damjan, AU - Kalamar,Bostjan, AU - Kreft,Samo, PY - 2012/8/23/entrez PY - 2012/8/23/pubmed PY - 2012/9/21/medline SP - 861 EP - 2 JF - Natural product communications JO - Nat Prod Commun VL - 7 IS - 7 N2 - An improved method for the isolation and purification of pyrrolizidine alkaloids from comfrey (Symphytum officinale L.) roots was developed, introducing very fast, selective and ion residue-free reduction of N-oxides followed by ion-exchange chromatography giving a non-aqueous solution of alkaloids, from which solvents can be easily removed. With this procedure the use of large volumes of organic solvents, very slow reduction of N-oxides and input of additional impurities was avoided. Lycopsamine, which proved to be the major alkaloid, was additionally purified by preparative layer chromatography (PLC) and high performance liquid chromatography (HPLC). The identity of the alkaloid was confirmed by (I)H NMR spectroscopy and mass spectrometry. SN - 1934-578X UR - https://www.unboundmedicine.com/medline/citation/22908565/Improved_method_for_isolation_of_lycopsamine_from_roots_of_comfrey__Symphytum_officinale__ L2 - https://www.lens.org/lens/search?q=citation_id:22908565 DB - PRIME DP - Unbound Medicine ER -