Tags

Type your tag names separated by a space and hit enter

Development of capsular polysaccharide-based glycoconjugates for immunization against melioidosis and glanders.

Abstract

Burkholderia pseudomallei and Burkholderia mallei, the etiologic agents of melioidosis and glanders, respectively, cause severe disease in humans and animals and are considered potential agents of biological warfare and terrorism. Diagnosis and treatment of infections caused by these pathogens can be challenging and, in the absence of chemotherapeutic intervention, acute disease is frequently fatal. At present, there are no human or veterinary vaccines available for immunization against these emerging/re-emerging infectious diseases. One of the long term objectives of our research, therefore, is to identify and characterize protective antigens expressed by B. pseudomallei and B. mallei and use them to develop efficacious vaccine candidates. Previous studies have demonstrated that the 6-deoxy-heptan capsular polysaccharide (CPS) expressed by these bacterial pathogens is both a virulence determinant and a protective antigen. Consequently, this carbohydrate moiety has become an important component of the various subunit vaccines that we are currently developing in our laboratory. In the present study, we describe a reliable method for isolating CPS antigens from O-polysaccharide (OPS) deficient strains of B. pseudomallei; including a derivative of the select agent excluded strain Bp82. Utilizing these purified CPS samples, we also describe a simple procedure for covalently linking these T-cell independent antigens to carrier proteins. In addition, we demonstrate that high titer IgG responses can be raised against the CPS component of such constructs. Collectively, these approaches provide a tangible starting point for the development of novel CPS-based glycoconjugates for immunization against melioidosis and glanders.

Authors+Show Affiliations

Department of Microbiology and Immunology, University of South Alabama Mobile, AL, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22912938

Citation

Burtnick, Mary N., et al. "Development of Capsular Polysaccharide-based Glycoconjugates for Immunization Against Melioidosis and Glanders." Frontiers in Cellular and Infection Microbiology, vol. 2, 2012, p. 108.
Burtnick MN, Heiss C, Roberts RA, et al. Development of capsular polysaccharide-based glycoconjugates for immunization against melioidosis and glanders. Front Cell Infect Microbiol. 2012;2:108.
Burtnick, M. N., Heiss, C., Roberts, R. A., Schweizer, H. P., Azadi, P., & Brett, P. J. (2012). Development of capsular polysaccharide-based glycoconjugates for immunization against melioidosis and glanders. Frontiers in Cellular and Infection Microbiology, 2, 108. https://doi.org/10.3389/fcimb.2012.00108
Burtnick MN, et al. Development of Capsular Polysaccharide-based Glycoconjugates for Immunization Against Melioidosis and Glanders. Front Cell Infect Microbiol. 2012;2:108. PubMed PMID: 22912938.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of capsular polysaccharide-based glycoconjugates for immunization against melioidosis and glanders. AU - Burtnick,Mary N, AU - Heiss,Christian, AU - Roberts,Rosemary A, AU - Schweizer,Herbert P, AU - Azadi,Parastoo, AU - Brett,Paul J, Y1 - 2012/08/15/ PY - 2012/06/25/received PY - 2012/07/28/accepted PY - 2012/8/23/entrez PY - 2012/8/23/pubmed PY - 2012/8/23/medline KW - Burkholderia mallei KW - Burkholderia pseudomallei KW - capsular polysaccharide KW - glycoconjugate KW - immunization KW - vaccine SP - 108 EP - 108 JF - Frontiers in cellular and infection microbiology JO - Front Cell Infect Microbiol VL - 2 N2 - Burkholderia pseudomallei and Burkholderia mallei, the etiologic agents of melioidosis and glanders, respectively, cause severe disease in humans and animals and are considered potential agents of biological warfare and terrorism. Diagnosis and treatment of infections caused by these pathogens can be challenging and, in the absence of chemotherapeutic intervention, acute disease is frequently fatal. At present, there are no human or veterinary vaccines available for immunization against these emerging/re-emerging infectious diseases. One of the long term objectives of our research, therefore, is to identify and characterize protective antigens expressed by B. pseudomallei and B. mallei and use them to develop efficacious vaccine candidates. Previous studies have demonstrated that the 6-deoxy-heptan capsular polysaccharide (CPS) expressed by these bacterial pathogens is both a virulence determinant and a protective antigen. Consequently, this carbohydrate moiety has become an important component of the various subunit vaccines that we are currently developing in our laboratory. In the present study, we describe a reliable method for isolating CPS antigens from O-polysaccharide (OPS) deficient strains of B. pseudomallei; including a derivative of the select agent excluded strain Bp82. Utilizing these purified CPS samples, we also describe a simple procedure for covalently linking these T-cell independent antigens to carrier proteins. In addition, we demonstrate that high titer IgG responses can be raised against the CPS component of such constructs. Collectively, these approaches provide a tangible starting point for the development of novel CPS-based glycoconjugates for immunization against melioidosis and glanders. SN - 2235-2988 UR - https://www.unboundmedicine.com/medline/citation/22912938/Development_of_capsular_polysaccharide_based_glycoconjugates_for_immunization_against_melioidosis_and_glanders_ L2 - https://doi.org/10.3389/fcimb.2012.00108 DB - PRIME DP - Unbound Medicine ER -