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Antimicrobial and antioxidant activities of the extracts and compounds from the leaves of Psorospermum aurantiacum Engl. and Hypericum lanceolatum Lam.
BMC Complement Altern Med. 2012 Aug 24; 12:136.BC

Abstract

BACKGROUND

Psorospermun aurantiacum and Hypericum lanceolatum are plants locally used in Cameroon and other parts of Africa for the treatment of gastrointestinal and urinary tract infections, skin infections, venereal diseases, gastrointestinal disorder, infertility, epilepsy as well as microbial infections. The present study was designed in order to investigate the in vitro antimicrobial and radical scavenging activities of the extracts and isolated compounds from the leaves of these plants.

METHODS

The plant extract was prepared by maceration in ethyl acetate and methanol and fractionated by column chromatography. The structures of isolated compounds were elucidated by spectroscopic analyses in conjunction with literature data. The broth microdilution method was used to evaluate the in vitro antimicrobial activity against bacteria, yeasts and dermatophytes. The antioxidant potentials of the extracts and their isolated compounds were evaluated using the DPPH radical scavenging method.

RESULTS

Five known compounds: physcion (1), 1,8-dihydroxy-3-geranyloxy-6-methylanthraquinone (2), kenganthranol B (3), vismiaquinone (4), and octacosanol (5) were isolated from the leaves of P. aurantiacum while six compounds including friedelin (6), betulinic acid (7), 2,2',5,6'-tetrahydroxybenzophenone (8), allanxanthone A (9), 1,3,6- trihydroxyxanthone (10) and isogarcinol (11) were isolated from H. lanceolatum. Compound 8 and 4 exhibited the highest antibacterial and antifungal activities with MIC ranges of 2-8 μg/ml and 4-32 μg/ml respectively. P. aurantiacum crude extract (Rsa50 = 6.359 ± 0.101) showed greater radical scavenging activity compared with H. lanceolatum extract (Rsa50 = 30.996 ± 0.879). Compound 11 showed the highest radical scavenging activity (RSa50 = 1.012 ± 0.247) among the isolated compounds, comparable to that of L-arscobic acid (RSa50 = 0.0809 ± 0.045).

CONCLUSIONS

The experimental findings show that the ethyl acetate and methanol extracts and isolated compounds from P. aurantiacum and H. lanceolatum stem bark possess significant antimicrobial and antioxidant activities justifying the use of these plants in traditional medicine, which may be developed as phytomedicines.

Authors+Show Affiliations

Laboratory of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22916964

Citation

Tchakam, Patricia D., et al. "Antimicrobial and Antioxidant Activities of the Extracts and Compounds From the Leaves of Psorospermum Aurantiacum Engl. and Hypericum Lanceolatum Lam." BMC Complementary and Alternative Medicine, vol. 12, 2012, p. 136.
Tchakam PD, Lunga PK, Kowa TK, et al. Antimicrobial and antioxidant activities of the extracts and compounds from the leaves of Psorospermum aurantiacum Engl. and Hypericum lanceolatum Lam. BMC Complement Altern Med. 2012;12:136.
Tchakam, P. D., Lunga, P. K., Kowa, T. K., Lonfouo, A. H., Wabo, H. K., Tapondjou, L. A., Tane, P., & Kuiate, J. R. (2012). Antimicrobial and antioxidant activities of the extracts and compounds from the leaves of Psorospermum aurantiacum Engl. and Hypericum lanceolatum Lam. BMC Complementary and Alternative Medicine, 12, 136. https://doi.org/10.1186/1472-6882-12-136
Tchakam PD, et al. Antimicrobial and Antioxidant Activities of the Extracts and Compounds From the Leaves of Psorospermum Aurantiacum Engl. and Hypericum Lanceolatum Lam. BMC Complement Altern Med. 2012 Aug 24;12:136. PubMed PMID: 22916964.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial and antioxidant activities of the extracts and compounds from the leaves of Psorospermum aurantiacum Engl. and Hypericum lanceolatum Lam. AU - Tchakam,Patricia D, AU - Lunga,Paul K, AU - Kowa,Théodora K, AU - Lonfouo,Antoine Honoré N, AU - Wabo,Hippolyte K, AU - Tapondjou,Léon A, AU - Tane,Pierre, AU - Kuiate,Jules-Roger, Y1 - 2012/08/24/ PY - 2012/03/15/received PY - 2012/08/14/accepted PY - 2012/8/25/entrez PY - 2012/8/25/pubmed PY - 2013/8/6/medline SP - 136 EP - 136 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 12 N2 - BACKGROUND: Psorospermun aurantiacum and Hypericum lanceolatum are plants locally used in Cameroon and other parts of Africa for the treatment of gastrointestinal and urinary tract infections, skin infections, venereal diseases, gastrointestinal disorder, infertility, epilepsy as well as microbial infections. The present study was designed in order to investigate the in vitro antimicrobial and radical scavenging activities of the extracts and isolated compounds from the leaves of these plants. METHODS: The plant extract was prepared by maceration in ethyl acetate and methanol and fractionated by column chromatography. The structures of isolated compounds were elucidated by spectroscopic analyses in conjunction with literature data. The broth microdilution method was used to evaluate the in vitro antimicrobial activity against bacteria, yeasts and dermatophytes. The antioxidant potentials of the extracts and their isolated compounds were evaluated using the DPPH radical scavenging method. RESULTS: Five known compounds: physcion (1), 1,8-dihydroxy-3-geranyloxy-6-methylanthraquinone (2), kenganthranol B (3), vismiaquinone (4), and octacosanol (5) were isolated from the leaves of P. aurantiacum while six compounds including friedelin (6), betulinic acid (7), 2,2',5,6'-tetrahydroxybenzophenone (8), allanxanthone A (9), 1,3,6- trihydroxyxanthone (10) and isogarcinol (11) were isolated from H. lanceolatum. Compound 8 and 4 exhibited the highest antibacterial and antifungal activities with MIC ranges of 2-8 μg/ml and 4-32 μg/ml respectively. P. aurantiacum crude extract (Rsa50 = 6.359 ± 0.101) showed greater radical scavenging activity compared with H. lanceolatum extract (Rsa50 = 30.996 ± 0.879). Compound 11 showed the highest radical scavenging activity (RSa50 = 1.012 ± 0.247) among the isolated compounds, comparable to that of L-arscobic acid (RSa50 = 0.0809 ± 0.045). CONCLUSIONS: The experimental findings show that the ethyl acetate and methanol extracts and isolated compounds from P. aurantiacum and H. lanceolatum stem bark possess significant antimicrobial and antioxidant activities justifying the use of these plants in traditional medicine, which may be developed as phytomedicines. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/22916964/Antimicrobial_and_antioxidant_activities_of_the_extracts_and_compounds_from_the_leaves_of_Psorospermum_aurantiacum_Engl__and_Hypericum_lanceolatum_Lam_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-12-136 DB - PRIME DP - Unbound Medicine ER -