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Characterization of polymethoxylated flavonoids (PMFs) in the peels of 'Shatangju' mandarin (Citrus reticulata Blanco) by online high-performance liquid chromatography coupled to photodiode array detection and electrospray tandem mass spectrometry.
J Agric Food Chem. 2012 Sep 12; 60(36):9023-34.JA

Abstract

A sensitive HPLC-DAD-ESI-MS/MS method was established to screen and identify the polymethoxylated flavonoids (PMFs) in the peels of 'Shatangju' mandarin (Citrus reticulata Blanco). Eight PMF standards, including four polymethoxylated flavones, two polymethoxylated flavanones, and two polymethoxylated chalcones, were first to be analyzed in positive mode by CID-MS/MS. On the basis of the ESI-MS(n) characteristics of PMFs and the results of EIC-MS/MS experiment, 32 PMFs including 24 flavones and 8 flavanones or chalcones were screened from the complex extract of the peels of 'Shatangju' mandarin. Among them, 10 PMFs were hydroxylated polymethoxyflavonoids (OH-PMFs), and the rest were all permethoxylated PMFs. This was the first systematic report of the presence of PMFs in the peels of 'Shatangju' mandarin, especially for polymethoxylated flavanones and chalcones. Meanwhile, the contents of the three main PMFs and total flavonoids in the peels of 'Shatangju' were determined by HPLC and UV spectrophotometry, respectively. The results indicated that the developed analytical method could be employed as an effective technique for the characterization of PMFs.

Authors+Show Affiliations

School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100029, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

22917253

Citation

Zhang, Jia-Yu, et al. "Characterization of Polymethoxylated Flavonoids (PMFs) in the Peels of 'Shatangju' Mandarin (Citrus Reticulata Blanco) By Online High-performance Liquid Chromatography Coupled to Photodiode Array Detection and Electrospray Tandem Mass Spectrometry." Journal of Agricultural and Food Chemistry, vol. 60, no. 36, 2012, pp. 9023-34.
Zhang JY, Zhang Q, Zhang HX, et al. Characterization of polymethoxylated flavonoids (PMFs) in the peels of 'Shatangju' mandarin (Citrus reticulata Blanco) by online high-performance liquid chromatography coupled to photodiode array detection and electrospray tandem mass spectrometry. J Agric Food Chem. 2012;60(36):9023-34.
Zhang, J. Y., Zhang, Q., Zhang, H. X., Ma, Q., Lu, J. Q., & Qiao, Y. J. (2012). Characterization of polymethoxylated flavonoids (PMFs) in the peels of 'Shatangju' mandarin (Citrus reticulata Blanco) by online high-performance liquid chromatography coupled to photodiode array detection and electrospray tandem mass spectrometry. Journal of Agricultural and Food Chemistry, 60(36), 9023-34. https://doi.org/10.1021/jf302713c
Zhang JY, et al. Characterization of Polymethoxylated Flavonoids (PMFs) in the Peels of 'Shatangju' Mandarin (Citrus Reticulata Blanco) By Online High-performance Liquid Chromatography Coupled to Photodiode Array Detection and Electrospray Tandem Mass Spectrometry. J Agric Food Chem. 2012 Sep 12;60(36):9023-34. PubMed PMID: 22917253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of polymethoxylated flavonoids (PMFs) in the peels of 'Shatangju' mandarin (Citrus reticulata Blanco) by online high-performance liquid chromatography coupled to photodiode array detection and electrospray tandem mass spectrometry. AU - Zhang,Jia-Yu, AU - Zhang,Qian, AU - Zhang,Hong-Xia, AU - Ma,Qun, AU - Lu,Jian-Qiu, AU - Qiao,Yan-Jiang, Y1 - 2012/09/04/ PY - 2012/8/25/entrez PY - 2012/8/25/pubmed PY - 2013/1/25/medline SP - 9023 EP - 34 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 60 IS - 36 N2 - A sensitive HPLC-DAD-ESI-MS/MS method was established to screen and identify the polymethoxylated flavonoids (PMFs) in the peels of 'Shatangju' mandarin (Citrus reticulata Blanco). Eight PMF standards, including four polymethoxylated flavones, two polymethoxylated flavanones, and two polymethoxylated chalcones, were first to be analyzed in positive mode by CID-MS/MS. On the basis of the ESI-MS(n) characteristics of PMFs and the results of EIC-MS/MS experiment, 32 PMFs including 24 flavones and 8 flavanones or chalcones were screened from the complex extract of the peels of 'Shatangju' mandarin. Among them, 10 PMFs were hydroxylated polymethoxyflavonoids (OH-PMFs), and the rest were all permethoxylated PMFs. This was the first systematic report of the presence of PMFs in the peels of 'Shatangju' mandarin, especially for polymethoxylated flavanones and chalcones. Meanwhile, the contents of the three main PMFs and total flavonoids in the peels of 'Shatangju' were determined by HPLC and UV spectrophotometry, respectively. The results indicated that the developed analytical method could be employed as an effective technique for the characterization of PMFs. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/22917253/Characterization_of_polymethoxylated_flavonoids__PMFs__in_the_peels_of_'Shatangju'_mandarin__Citrus_reticulata_Blanco__by_online_high_performance_liquid_chromatography_coupled_to_photodiode_array_detection_and_electrospray_tandem_mass_spectrometry_ L2 - https://doi.org/10.1021/jf302713c DB - PRIME DP - Unbound Medicine ER -