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A label-free electrochemiluminescence aptasensor for thrombin based on novel assembly strategy of oligonucleotide and luminol functionalized gold nanoparticles.
Biosens Bioelectron. 2013 Jan 15; 39(1):261-7.BB

Abstract

In the work, a label-free electrochemiluminescence (ECL) aptasensor for the sensitive and selective detection of thrombin was constructed based on target-induced direct ECL signal change by virtue of a novel assembly strategy of oligonucleotide and luminol functionalized gold nanoparticles (luminol-AuNPs). It is the first label-free ECL biosensor based on luminol and its analogs functionalized AuNPs. Streptavidin AuNPs coated with biotinylated DNA capture probe 1 (AuNPs-probe 1) were firstly assembled onto an gold electrode through 1,3-propanedithiol. Then luminol-AuNPs co-loaded with thiolated DNA capture probe 2 and thiolated thrombin binding aptamer (TBA) (luminol-AuNPs-probe 2/TBA) were assembled onto AuNPs-probe 1 modified electrode through the hybridization between capture probes 1 and 2. The luminol-AuNPs-probe 2/TBA acted as both molecule recognition probe and sensing interface. An Au/AuNPs/ds-DNA/luminol-AuNPs/TBA multilayer architecture was obtained. In the presence of target thrombin, TBA on the luminol-AuNPs could capture the thrombin onto the electrode surface, which produced a barrier for electro-transfer and influenced the electro-oxidation reaction of luminol, leading to a decrease in ECL intensity. The change of ECL intensity indirectly reflected the concentration of thrombin. Thus, the approach showed a high sensitivity and a wider linearity for the detection of thrombin in the range of 0.005-50nM with a detection limit of 1.7pM. This work reveals that luminol-AuNPs are ideal platform for label-free ECL bioassays.

Authors+Show Affiliations

CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, PR China.No affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22917918

Citation

Li, Fang, and Hua Cui. "A Label-free Electrochemiluminescence Aptasensor for Thrombin Based On Novel Assembly Strategy of Oligonucleotide and Luminol Functionalized Gold Nanoparticles." Biosensors & Bioelectronics, vol. 39, no. 1, 2013, pp. 261-7.
Li F, Cui H. A label-free electrochemiluminescence aptasensor for thrombin based on novel assembly strategy of oligonucleotide and luminol functionalized gold nanoparticles. Biosens Bioelectron. 2013;39(1):261-7.
Li, F., & Cui, H. (2013). A label-free electrochemiluminescence aptasensor for thrombin based on novel assembly strategy of oligonucleotide and luminol functionalized gold nanoparticles. Biosensors & Bioelectronics, 39(1), 261-7. https://doi.org/10.1016/j.bios.2012.07.060
Li F, Cui H. A Label-free Electrochemiluminescence Aptasensor for Thrombin Based On Novel Assembly Strategy of Oligonucleotide and Luminol Functionalized Gold Nanoparticles. Biosens Bioelectron. 2013 Jan 15;39(1):261-7. PubMed PMID: 22917918.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A label-free electrochemiluminescence aptasensor for thrombin based on novel assembly strategy of oligonucleotide and luminol functionalized gold nanoparticles. AU - Li,Fang, AU - Cui,Hua, Y1 - 2012/08/09/ PY - 2012/05/22/received PY - 2012/07/08/revised PY - 2012/07/27/accepted PY - 2012/8/25/entrez PY - 2012/8/25/pubmed PY - 2013/1/29/medline SP - 261 EP - 7 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 39 IS - 1 N2 - In the work, a label-free electrochemiluminescence (ECL) aptasensor for the sensitive and selective detection of thrombin was constructed based on target-induced direct ECL signal change by virtue of a novel assembly strategy of oligonucleotide and luminol functionalized gold nanoparticles (luminol-AuNPs). It is the first label-free ECL biosensor based on luminol and its analogs functionalized AuNPs. Streptavidin AuNPs coated with biotinylated DNA capture probe 1 (AuNPs-probe 1) were firstly assembled onto an gold electrode through 1,3-propanedithiol. Then luminol-AuNPs co-loaded with thiolated DNA capture probe 2 and thiolated thrombin binding aptamer (TBA) (luminol-AuNPs-probe 2/TBA) were assembled onto AuNPs-probe 1 modified electrode through the hybridization between capture probes 1 and 2. The luminol-AuNPs-probe 2/TBA acted as both molecule recognition probe and sensing interface. An Au/AuNPs/ds-DNA/luminol-AuNPs/TBA multilayer architecture was obtained. In the presence of target thrombin, TBA on the luminol-AuNPs could capture the thrombin onto the electrode surface, which produced a barrier for electro-transfer and influenced the electro-oxidation reaction of luminol, leading to a decrease in ECL intensity. The change of ECL intensity indirectly reflected the concentration of thrombin. Thus, the approach showed a high sensitivity and a wider linearity for the detection of thrombin in the range of 0.005-50nM with a detection limit of 1.7pM. This work reveals that luminol-AuNPs are ideal platform for label-free ECL bioassays. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/22917918/A_label_free_electrochemiluminescence_aptasensor_for_thrombin_based_on_novel_assembly_strategy_of_oligonucleotide_and_luminol_functionalized_gold_nanoparticles_ DB - PRIME DP - Unbound Medicine ER -