Tags

Type your tag names separated by a space and hit enter

Antimicrobial effect of conventional root canal medicaments vs propolis against Enterococcus faecalis, Staphylococcus aureus and Candida albicans.
J Contemp Dent Pract. 2012 May 01; 13(3):305-9.JC

Abstract

AIM

To evaluate and compare antimicrobial effect of various root canal medicaments against Enterococcus faecalis, Staphylococcus aureus and Candida albicans.

MATERIALS AND METHODS

Six root canal medicaments: 2% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) Calcium hydroxide (Ca(OH)2), EDTA, MTAD and propolis and three microorganisms: Staphylococcus aureus, Enterococcus faecalis and Candida albicans were used. These strains were inoculated in brain heart infusion (BHI) and incubated at 37 degrees C for 24 hours. For the agar diffusion test (ADT), petri plates with 20 ml of BHI agar were inoculated with 0.1 ml of the microbial suspensions, using sterile swabs that were spread on the medium, obtaining growth injunction. Paper disks were immersed in the experimental solutions for 1 minute. Subsequently, four papers disks containing one of the substances were placed on the BHI agar surface in each agar plate. The plates were incubated at 37°C for 48 hours. The diameter of microbial inhibition was measured around the papers disks containing the substances. One way ANOVA followed by Tukey's post-hoc test were used. p-value >0.05 was considered statistically significant.

RESULTS

Propolis and other irrigants were found to be effective on C. albicans, S. aureus and E. faecalis. CHX and MTAD were found to be most effective amongst all the materials tested followed by propolis.

CONCLUSION

Propolis showed antimicrobial activity against E. faecalis, S. aureus, C. albicans. It appears that propolis is an effective intracanal irrigant in eradicating E. faecalis and C. albicans.

CLINICAL SIGNIFICANCE

Propolis is an effective intracanal irrigant in eradicating E. faecalis and C. albicans. It could be used as an alternative intracanal medicament.

Authors+Show Affiliations

Reader, Department of Conservative Dentistry and Endodontics Krishna School of Dental Sciences, KIMSDU, Karad Satara, Maharashtra, India. sandeshphaphe@rediffmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

22918001

Citation

Mattigatti, Sudha, et al. "Antimicrobial Effect of Conventional Root Canal Medicaments Vs Propolis Against Enterococcus Faecalis, Staphylococcus Aureus and Candida Albicans." The Journal of Contemporary Dental Practice, vol. 13, no. 3, 2012, pp. 305-9.
Mattigatti S, Ratnakar P, Moturi S, et al. Antimicrobial effect of conventional root canal medicaments vs propolis against Enterococcus faecalis, Staphylococcus aureus and Candida albicans. J Contemp Dent Pract. 2012;13(3):305-9.
Mattigatti, S., Ratnakar, P., Moturi, S., Varma, S., & Rairam, S. (2012). Antimicrobial effect of conventional root canal medicaments vs propolis against Enterococcus faecalis, Staphylococcus aureus and Candida albicans. The Journal of Contemporary Dental Practice, 13(3), 305-9.
Mattigatti S, et al. Antimicrobial Effect of Conventional Root Canal Medicaments Vs Propolis Against Enterococcus Faecalis, Staphylococcus Aureus and Candida Albicans. J Contemp Dent Pract. 2012 May 1;13(3):305-9. PubMed PMID: 22918001.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial effect of conventional root canal medicaments vs propolis against Enterococcus faecalis, Staphylococcus aureus and Candida albicans. AU - Mattigatti,Sudha, AU - Ratnakar,P, AU - Moturi,Shridhar, AU - Varma,Sarath, AU - Rairam,Surabhi, Y1 - 2012/05/01/ PY - 2012/8/25/entrez PY - 2012/8/25/pubmed PY - 2013/3/19/medline SP - 305 EP - 9 JF - The journal of contemporary dental practice JO - J Contemp Dent Pract VL - 13 IS - 3 N2 - AIM: To evaluate and compare antimicrobial effect of various root canal medicaments against Enterococcus faecalis, Staphylococcus aureus and Candida albicans. MATERIALS AND METHODS: Six root canal medicaments: 2% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) Calcium hydroxide (Ca(OH)2), EDTA, MTAD and propolis and three microorganisms: Staphylococcus aureus, Enterococcus faecalis and Candida albicans were used. These strains were inoculated in brain heart infusion (BHI) and incubated at 37 degrees C for 24 hours. For the agar diffusion test (ADT), petri plates with 20 ml of BHI agar were inoculated with 0.1 ml of the microbial suspensions, using sterile swabs that were spread on the medium, obtaining growth injunction. Paper disks were immersed in the experimental solutions for 1 minute. Subsequently, four papers disks containing one of the substances were placed on the BHI agar surface in each agar plate. The plates were incubated at 37°C for 48 hours. The diameter of microbial inhibition was measured around the papers disks containing the substances. One way ANOVA followed by Tukey's post-hoc test were used. p-value >0.05 was considered statistically significant. RESULTS: Propolis and other irrigants were found to be effective on C. albicans, S. aureus and E. faecalis. CHX and MTAD were found to be most effective amongst all the materials tested followed by propolis. CONCLUSION: Propolis showed antimicrobial activity against E. faecalis, S. aureus, C. albicans. It appears that propolis is an effective intracanal irrigant in eradicating E. faecalis and C. albicans. CLINICAL SIGNIFICANCE: Propolis is an effective intracanal irrigant in eradicating E. faecalis and C. albicans. It could be used as an alternative intracanal medicament. SN - 1526-3711 UR - https://www.unboundmedicine.com/medline/citation/22918001/Antimicrobial_effect_of_conventional_root_canal_medicaments_vs_propolis_against_Enterococcus_faecalis_Staphylococcus_aureus_and_Candida_albicans_ DB - PRIME DP - Unbound Medicine ER -