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Eutopic endometrium and peritoneal, ovarian and colorectal endometriotic tissues express a different profile of nectin-1, -3, -4 and nectin-like molecule 2.
Hum Reprod. 2012 Nov; 27(11):3179-86.HR

Abstract

STUDY QUESTION

How is the expression of nectins and nectin-like molecules (Necls) detected by immunostaining altered by endometriosis?

SUMMARY ANSWER

Our results suggest that Nectin-1, -3, -4 and Necl-2 may contribute to the pathogenesis of endometriosis. Immunostaining of nectins and Necls varies according to the anatomical location of endometriosis.

WHAT IS KNOWN AND WHAT THIS PAPER ADDS

Nectin and Necl molecules are immunoglobulin-like cell adhesion molecules involved in apoptosis, cell proliferation and in metastases. Previous studies have demonstrated the involvement of adhesion molecules in the development of endometriotic lesions but no data exist on immunostaining of nectins and Necls molecules in endometriosis.

DESIGN, PARTICIPANTS AND SETTING

This retrospective study was conducted in a tertiary-care hospital (Tenon Hospital, Paris, France). Samples were collected from 55 women undergoing endometrial biopsy or surgery for endometriosis and 20 controls having hysterectomy or endometrial biopsy for other reasons; multiple samples were collected from 15 women. We studied the immunostaining of Nectin-1, -3, -4 and Necl-2 in secretory and proliferative endometrium from women with (n = 20) or without endometriosis (i.e. control group, n = 20), and in peritoneal (n = 20), ovarian (n = 20) and colorectal endometriosis (n = 20).

MAIN RESULTS

Semi-quantitative immunostaining demonstrated that (1) Necl-2 staining was stronger in all types of endometriotic lesions than in the eutopic endometrium from patients with endometriosis (P < 0.0125) and in ovarian endometriotic cysts compared with other locations (P < 0.001); (2) Nectin-3 staining was stronger in the eutopic endometrium of patients with endometriosis compared with controls (P = 0.03) and in all endometriotic lesions compared with the eutopic endometrium from patients with endometriosis (P < 0.0125); (3) Nectin-4, staining was stronger in the eutopic endometrium of patients with endometriosis compared with controls (P = 0.04) and (4) Nectin-1 staining was significantly increased in colorectal endometriosis compared with other locations (P = 0.004).

BIAS, CONFOUNDING AND OTHER REASONS FOR CAUTION

We did not assess the pattern of expression in endometriosis of all nectins and Necl molecules. Indeed, Necl-5 is implicated in many pathophysiological processes such as cell movement and proliferation with potential relevance to endometriosis. GENERALISABILITY TO OTHER POPULATIONS: At present, few data on implication of nectins and Necl molecules in endometriosis exist. Hence, our results should be confirmed by further quantitative studies at protein or RNA levels.

STUDY FUNDING/COMPETING INTEREST(S)

No funding source. All the authors declare no conflict of interest.

Authors+Show Affiliations

ER2 UPMC, Université Pierre et Marie Curie, Paris 6, France. marcos.ballester@tnn.aphp.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

22926846

Citation

Ballester, Marcos, et al. "Eutopic Endometrium and Peritoneal, Ovarian and Colorectal Endometriotic Tissues Express a Different Profile of Nectin-1, -3, -4 and Nectin-like Molecule 2." Human Reproduction (Oxford, England), vol. 27, no. 11, 2012, pp. 3179-86.
Ballester M, Gonin J, Rodenas A, et al. Eutopic endometrium and peritoneal, ovarian and colorectal endometriotic tissues express a different profile of nectin-1, -3, -4 and nectin-like molecule 2. Hum Reprod. 2012;27(11):3179-86.
Ballester, M., Gonin, J., Rodenas, A., Bernaudin, J. F., Rouzier, R., Coutant, C., & Daraï, E. (2012). Eutopic endometrium and peritoneal, ovarian and colorectal endometriotic tissues express a different profile of nectin-1, -3, -4 and nectin-like molecule 2. Human Reproduction (Oxford, England), 27(11), 3179-86. https://doi.org/10.1093/humrep/des304
Ballester M, et al. Eutopic Endometrium and Peritoneal, Ovarian and Colorectal Endometriotic Tissues Express a Different Profile of Nectin-1, -3, -4 and Nectin-like Molecule 2. Hum Reprod. 2012;27(11):3179-86. PubMed PMID: 22926846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Eutopic endometrium and peritoneal, ovarian and colorectal endometriotic tissues express a different profile of nectin-1, -3, -4 and nectin-like molecule 2. AU - Ballester,Marcos, AU - Gonin,Julie, AU - Rodenas,Anita, AU - Bernaudin,Jean-François, AU - Rouzier,Roman, AU - Coutant,Charles, AU - Daraï,Emile, Y1 - 2012/08/27/ PY - 2012/8/29/entrez PY - 2012/8/29/pubmed PY - 2013/3/30/medline SP - 3179 EP - 86 JF - Human reproduction (Oxford, England) JO - Hum. Reprod. VL - 27 IS - 11 N2 - STUDY QUESTION: How is the expression of nectins and nectin-like molecules (Necls) detected by immunostaining altered by endometriosis? SUMMARY ANSWER: Our results suggest that Nectin-1, -3, -4 and Necl-2 may contribute to the pathogenesis of endometriosis. Immunostaining of nectins and Necls varies according to the anatomical location of endometriosis. WHAT IS KNOWN AND WHAT THIS PAPER ADDS: Nectin and Necl molecules are immunoglobulin-like cell adhesion molecules involved in apoptosis, cell proliferation and in metastases. Previous studies have demonstrated the involvement of adhesion molecules in the development of endometriotic lesions but no data exist on immunostaining of nectins and Necls molecules in endometriosis. DESIGN, PARTICIPANTS AND SETTING: This retrospective study was conducted in a tertiary-care hospital (Tenon Hospital, Paris, France). Samples were collected from 55 women undergoing endometrial biopsy or surgery for endometriosis and 20 controls having hysterectomy or endometrial biopsy for other reasons; multiple samples were collected from 15 women. We studied the immunostaining of Nectin-1, -3, -4 and Necl-2 in secretory and proliferative endometrium from women with (n = 20) or without endometriosis (i.e. control group, n = 20), and in peritoneal (n = 20), ovarian (n = 20) and colorectal endometriosis (n = 20). MAIN RESULTS: Semi-quantitative immunostaining demonstrated that (1) Necl-2 staining was stronger in all types of endometriotic lesions than in the eutopic endometrium from patients with endometriosis (P < 0.0125) and in ovarian endometriotic cysts compared with other locations (P < 0.001); (2) Nectin-3 staining was stronger in the eutopic endometrium of patients with endometriosis compared with controls (P = 0.03) and in all endometriotic lesions compared with the eutopic endometrium from patients with endometriosis (P < 0.0125); (3) Nectin-4, staining was stronger in the eutopic endometrium of patients with endometriosis compared with controls (P = 0.04) and (4) Nectin-1 staining was significantly increased in colorectal endometriosis compared with other locations (P = 0.004). BIAS, CONFOUNDING AND OTHER REASONS FOR CAUTION: We did not assess the pattern of expression in endometriosis of all nectins and Necl molecules. Indeed, Necl-5 is implicated in many pathophysiological processes such as cell movement and proliferation with potential relevance to endometriosis. GENERALISABILITY TO OTHER POPULATIONS: At present, few data on implication of nectins and Necl molecules in endometriosis exist. Hence, our results should be confirmed by further quantitative studies at protein or RNA levels. STUDY FUNDING/COMPETING INTEREST(S): No funding source. All the authors declare no conflict of interest. SN - 1460-2350 UR - https://www.unboundmedicine.com/medline/citation/22926846/Eutopic_endometrium_and_peritoneal_ovarian_and_colorectal_endometriotic_tissues_express_a_different_profile_of_nectin_1__3__4_and_nectin_like_molecule_2_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/des304 DB - PRIME DP - Unbound Medicine ER -