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Incorporation of eicosapentaenoic and docosahexaenoic acids into lipid pools when given as supplements providing doses equivalent to typical intakes of oily fish.
Am J Clin Nutr. 2012 Oct; 96(4):748-58.AJ

Abstract

BACKGROUND

Estimation of the intake of oily fish at a population level is difficult. The measurement of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in biological samples may provide a useful biomarker of intake.

OBJECTIVE

We identified the most appropriate biomarkers for the assessment of habitual oily fish intake and changes in intake by elucidating the dose- and time-dependent response of EPA and DHA incorporation into various biological samples that represent roles in fatty acid transport, function, and storage.

DESIGN

This was a double-blind, randomized, controlled intervention trial in 204 men and women that lasted 12 mo. EPA and DHA capsules were provided in a manner to reflect sporadic consumption of oily fish (ie, 1, 2, or 4 times/wk). EPA and DHA were assessed at 9 time points over 12 mo in 9 sample types (red blood cells, mononuclear cells, platelets, buccal cells, adipose tissue, plasma phosphatidylcholine, triglycerides, cholesteryl esters, and nonesterified fatty acids).

RESULTS

A dose response (P < 0.05) was observed for EPA and DHA in all pools except for red blood cell EPA (P = 0.057). EPA and DHA measures in plasma phosphatidylcholine and platelets were best for the discrimination between different intakes (P < 0.0001). The rate of incorporation varied between sample types, with the time to maximal incorporation ranging from days (plasma phosphatidylcholine) to months (mononuclear cells) to >12 mo (adipose tissue).

CONCLUSIONS

Plasma phosphatidylcholine EPA plus DHA was identified as the most suitable biomarker of acute changes in EPA and DHA intake, and platelet and mononuclear cell EPA plus DHA were the most suitable biomarkers of habitual intake.

Authors+Show Affiliations

Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, Cambridge, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Multicenter Study
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22932281

Citation

Browning, Lucy M., et al. "Incorporation of Eicosapentaenoic and Docosahexaenoic Acids Into Lipid Pools when Given as Supplements Providing Doses Equivalent to Typical Intakes of Oily Fish." The American Journal of Clinical Nutrition, vol. 96, no. 4, 2012, pp. 748-58.
Browning LM, Walker CG, Mander AP, et al. Incorporation of eicosapentaenoic and docosahexaenoic acids into lipid pools when given as supplements providing doses equivalent to typical intakes of oily fish. Am J Clin Nutr. 2012;96(4):748-58.
Browning, L. M., Walker, C. G., Mander, A. P., West, A. L., Madden, J., Gambell, J. M., Young, S., Wang, L., Jebb, S. A., & Calder, P. C. (2012). Incorporation of eicosapentaenoic and docosahexaenoic acids into lipid pools when given as supplements providing doses equivalent to typical intakes of oily fish. The American Journal of Clinical Nutrition, 96(4), 748-58.
Browning LM, et al. Incorporation of Eicosapentaenoic and Docosahexaenoic Acids Into Lipid Pools when Given as Supplements Providing Doses Equivalent to Typical Intakes of Oily Fish. Am J Clin Nutr. 2012;96(4):748-58. PubMed PMID: 22932281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Incorporation of eicosapentaenoic and docosahexaenoic acids into lipid pools when given as supplements providing doses equivalent to typical intakes of oily fish. AU - Browning,Lucy M, AU - Walker,Celia G, AU - Mander,Adrian P, AU - West,Annette L, AU - Madden,Jackie, AU - Gambell,Joanna M, AU - Young,Stephen, AU - Wang,Laura, AU - Jebb,Susan A, AU - Calder,Philip C, Y1 - 2012/08/29/ PY - 2012/8/31/entrez PY - 2012/8/31/pubmed PY - 2012/12/12/medline SP - 748 EP - 58 JF - The American journal of clinical nutrition JO - Am J Clin Nutr VL - 96 IS - 4 N2 - BACKGROUND: Estimation of the intake of oily fish at a population level is difficult. The measurement of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in biological samples may provide a useful biomarker of intake. OBJECTIVE: We identified the most appropriate biomarkers for the assessment of habitual oily fish intake and changes in intake by elucidating the dose- and time-dependent response of EPA and DHA incorporation into various biological samples that represent roles in fatty acid transport, function, and storage. DESIGN: This was a double-blind, randomized, controlled intervention trial in 204 men and women that lasted 12 mo. EPA and DHA capsules were provided in a manner to reflect sporadic consumption of oily fish (ie, 1, 2, or 4 times/wk). EPA and DHA were assessed at 9 time points over 12 mo in 9 sample types (red blood cells, mononuclear cells, platelets, buccal cells, adipose tissue, plasma phosphatidylcholine, triglycerides, cholesteryl esters, and nonesterified fatty acids). RESULTS: A dose response (P < 0.05) was observed for EPA and DHA in all pools except for red blood cell EPA (P = 0.057). EPA and DHA measures in plasma phosphatidylcholine and platelets were best for the discrimination between different intakes (P < 0.0001). The rate of incorporation varied between sample types, with the time to maximal incorporation ranging from days (plasma phosphatidylcholine) to months (mononuclear cells) to >12 mo (adipose tissue). CONCLUSIONS: Plasma phosphatidylcholine EPA plus DHA was identified as the most suitable biomarker of acute changes in EPA and DHA intake, and platelet and mononuclear cell EPA plus DHA were the most suitable biomarkers of habitual intake. SN - 1938-3207 UR - https://www.unboundmedicine.com/medline/citation/22932281/Incorporation_of_eicosapentaenoic_and_docosahexaenoic_acids_into_lipid_pools_when_given_as_supplements_providing_doses_equivalent_to_typical_intakes_of_oily_fish_ L2 - https://academic.oup.com/ajcn/article-lookup/doi/10.3945/ajcn.112.041343 DB - PRIME DP - Unbound Medicine ER -