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[Effects of electroacupuncture at "Futu"(LI 18), etc. on pain threshold and cervico-spinal mGlu receptor 5/cAmp/CREB signaling in rats with neck incision pain].
Zhen Ci Yan Jiu. 2012 Jun; 37(3):191-6.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) at "Futu" (LI 18),etc. on the pain threshold and expression of metabotropic glutamate receptor subtype 5 (mGluR 5), cyclic adenosine monophosphate (cAMP), mitogen-activated protein kinase (MAPK), cAMP responsive element binding protein (CREB) genes in the cervico-spinal dorsal region in rats with neck incision pain so as to analyze its underlying analgesic mechanism in performing thyroid surgery.

METHODS

Forty male Wistar rats were randomly divided into control, model, Futu (LI 18), Hegu (LI 4)-Neiguan (PC 6), Zusanli (ST 36)-Yanglingquan (GB 34) groups, with 8 cases in each group. Neck-incision pain model was established by making a longitudinal incision along the midline of the neck under anesthesia (isoflurane) except rats in the control group. EA(2 Hz/15 Hz, 1-2 mA) was applied to bilateral LI 18, LI 4-PC 6 and ST 36-GB 34 for 30 min, 4 h, 24 h and 48 h after modeling, respectively. The thermal pain threshold was detected before modeling, and before and after each treatment. After removing the ventral part, the spinal tissue of the cervical 1 -4 segments was collected for detecting the expression of mGluR 5, cAMP, MAPK, CREB genes by using quantitative real-time-PCR technique.

RESULTS

In comparison with the control group, the pain threshold of the model group was decreased obviously (P<0.05). Compared with the model group, the pain thresholds of the LI 18 and LI 4-PC 6 groups were upregulated markedly after treatment (P<0.05). No significant differences were found between the LI 18 and LI 4-PC 6 groups, and between the model and ST 36-GB 34 groups in pain thresholds (P>0.05). Compared with the control group, the expression le-vels of mGluR 5 mRNA, cAMP mRNA and CREB mRNA in the cervico-spinal cord were upregulated significantly in the model group (P<0.05). While in comparison with the model group, cervico-spinal cAMP mRNA, and CREB mRNA were down-regulated markedly (P<0.05) and mGluR 5 mRNA was down-regulated moderately (P>0.05) in the LI 18 group, rather than in the LI 4-PC 6 and ST 36-GB 34 groups (P>0.05).

CONCLUSION

EA-LI 18 can significantly suppress neck incision pain induced pain reaction, which may be closely associated with its functions in down-regulating expression levels of intracellular cAMP mRNA and CREB mRNA in the cervico-spinal cord.

Authors+Show Affiliations

Lin D
Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Kan Y
No affiliation info available
Qiao LN
No affiliation info available
Wang JY
No affiliation info available
Chen SP
No affiliation info available
Han YJ
No affiliation info available
Zhang JL
No affiliation info available
Liu JL
No affiliation info available

MeSH

Acupuncture PointsAnimalsCervical VertebraeCyclic AMPCyclic AMP Response Element-Binding ProteinElectroacupunctureGene Expression RegulationMaleMitogen-Activated Protein KinasesNeck PainPain ThresholdRatsRats, WistarReceptor, Metabotropic Glutamate 5Receptors, Metabotropic GlutamateSignal Transduction

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

22934388

Citation

Lin, Dan, et al. "[Effects of Electroacupuncture at "Futu"(LI 18), Etc. On Pain Threshold and Cervico-spinal mGlu Receptor 5/cAmp/CREB Signaling in Rats With Neck Incision Pain]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 37, no. 3, 2012, pp. 191-6.
Lin D, Kan Y, Qiao LN, et al. [Effects of electroacupuncture at "Futu"(LI 18), etc. on pain threshold and cervico-spinal mGlu receptor 5/cAmp/CREB signaling in rats with neck incision pain]. Zhen Ci Yan Jiu. 2012;37(3):191-6.
Lin, D., Kan, Y., Qiao, L. N., Wang, J. Y., Chen, S. P., Han, Y. J., Zhang, J. L., & Liu, J. L. (2012). [Effects of electroacupuncture at "Futu"(LI 18), etc. on pain threshold and cervico-spinal mGlu receptor 5/cAmp/CREB signaling in rats with neck incision pain]. Zhen Ci Yan Jiu = Acupuncture Research, 37(3), 191-6.
Lin D, et al. [Effects of Electroacupuncture at "Futu"(LI 18), Etc. On Pain Threshold and Cervico-spinal mGlu Receptor 5/cAmp/CREB Signaling in Rats With Neck Incision Pain]. Zhen Ci Yan Jiu. 2012;37(3):191-6. PubMed PMID: 22934388.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of electroacupuncture at "Futu"(LI 18), etc. on pain threshold and cervico-spinal mGlu receptor 5/cAmp/CREB signaling in rats with neck incision pain]. AU - Lin,Dan, AU - Kan,Yu, AU - Qiao,Li-Na, AU - Wang,Jun-Ying, AU - Chen,Shu-Ping, AU - Han,Yan-Jing, AU - Zhang,Jian-Liang, AU - Liu,Jun-Ling, PY - 2012/9/1/entrez PY - 2012/9/1/pubmed PY - 2012/10/13/medline SP - 191 EP - 6 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 37 IS - 3 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) at "Futu" (LI 18),etc. on the pain threshold and expression of metabotropic glutamate receptor subtype 5 (mGluR 5), cyclic adenosine monophosphate (cAMP), mitogen-activated protein kinase (MAPK), cAMP responsive element binding protein (CREB) genes in the cervico-spinal dorsal region in rats with neck incision pain so as to analyze its underlying analgesic mechanism in performing thyroid surgery. METHODS: Forty male Wistar rats were randomly divided into control, model, Futu (LI 18), Hegu (LI 4)-Neiguan (PC 6), Zusanli (ST 36)-Yanglingquan (GB 34) groups, with 8 cases in each group. Neck-incision pain model was established by making a longitudinal incision along the midline of the neck under anesthesia (isoflurane) except rats in the control group. EA(2 Hz/15 Hz, 1-2 mA) was applied to bilateral LI 18, LI 4-PC 6 and ST 36-GB 34 for 30 min, 4 h, 24 h and 48 h after modeling, respectively. The thermal pain threshold was detected before modeling, and before and after each treatment. After removing the ventral part, the spinal tissue of the cervical 1 -4 segments was collected for detecting the expression of mGluR 5, cAMP, MAPK, CREB genes by using quantitative real-time-PCR technique. RESULTS: In comparison with the control group, the pain threshold of the model group was decreased obviously (P<0.05). Compared with the model group, the pain thresholds of the LI 18 and LI 4-PC 6 groups were upregulated markedly after treatment (P<0.05). No significant differences were found between the LI 18 and LI 4-PC 6 groups, and between the model and ST 36-GB 34 groups in pain thresholds (P>0.05). Compared with the control group, the expression le-vels of mGluR 5 mRNA, cAMP mRNA and CREB mRNA in the cervico-spinal cord were upregulated significantly in the model group (P<0.05). While in comparison with the model group, cervico-spinal cAMP mRNA, and CREB mRNA were down-regulated markedly (P<0.05) and mGluR 5 mRNA was down-regulated moderately (P>0.05) in the LI 18 group, rather than in the LI 4-PC 6 and ST 36-GB 34 groups (P>0.05). CONCLUSION: EA-LI 18 can significantly suppress neck incision pain induced pain reaction, which may be closely associated with its functions in down-regulating expression levels of intracellular cAMP mRNA and CREB mRNA in the cervico-spinal cord. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/22934388/[Effects_of_electroacupuncture_at_"Futu"_LI_18__etc__on_pain_threshold_and_cervico_spinal_mGlu_receptor_5/cAmp/CREB_signaling_in_rats_with_neck_incision_pain]_ L2 - https://medlineplus.gov/neckinjuriesanddisorders.html DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓4]

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