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Omega-3 polyunsaturated fatty acid profiling using fingertip-prick whole blood does not require overnight fasting before blood collection.
Nutr Res 2012; 32(8):547-56NR

Abstract

Fatty acid profiling through the rapid analysis of capillary blood collected by fingertip prick could enable economical screening for omega-3 polyunsaturated fatty acid (PUFA) status, although the typical requirement of fasting prior to sample collection may limit application. We hypothesize that moderate changes in omega-3 biomarkers determined from fingertip-prick blood will occur and correspond to omega-3 PUFA content of the meals. Eight participants consumed a single breakfast with high fat, high fat with omega-3 functional foods, and low fat and low fat with fish oil capsules in a cross-over design. The fatty acid composition of fingertip-prick blood total lipid and venous blood erythrocyte total lipid, plasma total lipid, plasma triacylglycerol, and plasma phospholipids were analyzed at baseline and 1, 2, 3 and 4 hours after each single breakfast consumption. Omega-3 blood biomarkers; % of omega-3 highly unsaturated fatty acid (HUFA) in total HUFA, weight % of eicosapentaenoic acid+docosahexaenoic acid, weight % of eicosapentaenoic acid+omega-3 docosapentaenoic acid+docosahexaenoic acid, and the ratio of total omega-6 PUFA to total omega-3 PUFA in fingertip-prick blood, did not change from baseline during the postprandial period (P > .05). However, meal type yielded lower (P < .05) % omega-3 HUFA in total HUFA in the low fat meal (22.8 ± 3.9) as compared with the low fat with omega-3 (24.2 ± 3.9) and, the high fat (23.8 ± 4) meals. The ratio of total omega-6 PUFA to total omega-3 PUFA was generally higher in meals without omega-3 compared with omega-3. In conclusion, determinations of omega-3 status by fingertip-prick blood sampling may not require prior overnight fasting.

Authors+Show Affiliations

Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22935337

Citation

Metherel, Adam H., et al. "Omega-3 Polyunsaturated Fatty Acid Profiling Using Fingertip-prick Whole Blood Does Not Require Overnight Fasting Before Blood Collection." Nutrition Research (New York, N.Y.), vol. 32, no. 8, 2012, pp. 547-56.
Metherel AH, Buzikievich LM, Charkhzarin P, et al. Omega-3 polyunsaturated fatty acid profiling using fingertip-prick whole blood does not require overnight fasting before blood collection. Nutr Res. 2012;32(8):547-56.
Metherel, A. H., Buzikievich, L. M., Charkhzarin, P., Patterson, A. C., Peel, A. C., Howorth, A. M., ... Stark, K. D. (2012). Omega-3 polyunsaturated fatty acid profiling using fingertip-prick whole blood does not require overnight fasting before blood collection. Nutrition Research (New York, N.Y.), 32(8), pp. 547-56. doi:10.1016/j.nutres.2012.06.016.
Metherel AH, et al. Omega-3 Polyunsaturated Fatty Acid Profiling Using Fingertip-prick Whole Blood Does Not Require Overnight Fasting Before Blood Collection. Nutr Res. 2012;32(8):547-56. PubMed PMID: 22935337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Omega-3 polyunsaturated fatty acid profiling using fingertip-prick whole blood does not require overnight fasting before blood collection. AU - Metherel,Adam H, AU - Buzikievich,Lindy M, AU - Charkhzarin,Payman, AU - Patterson,Ashley C, AU - Peel,Alexandrea C, AU - Howorth,Allison M, AU - Kishi,Diane M, AU - Stark,Ken D, Y1 - 2012/08/02/ PY - 2012/01/30/received PY - 2012/06/25/revised PY - 2012/06/28/accepted PY - 2012/9/1/entrez PY - 2012/9/1/pubmed PY - 2013/1/12/medline SP - 547 EP - 56 JF - Nutrition research (New York, N.Y.) JO - Nutr Res VL - 32 IS - 8 N2 - Fatty acid profiling through the rapid analysis of capillary blood collected by fingertip prick could enable economical screening for omega-3 polyunsaturated fatty acid (PUFA) status, although the typical requirement of fasting prior to sample collection may limit application. We hypothesize that moderate changes in omega-3 biomarkers determined from fingertip-prick blood will occur and correspond to omega-3 PUFA content of the meals. Eight participants consumed a single breakfast with high fat, high fat with omega-3 functional foods, and low fat and low fat with fish oil capsules in a cross-over design. The fatty acid composition of fingertip-prick blood total lipid and venous blood erythrocyte total lipid, plasma total lipid, plasma triacylglycerol, and plasma phospholipids were analyzed at baseline and 1, 2, 3 and 4 hours after each single breakfast consumption. Omega-3 blood biomarkers; % of omega-3 highly unsaturated fatty acid (HUFA) in total HUFA, weight % of eicosapentaenoic acid+docosahexaenoic acid, weight % of eicosapentaenoic acid+omega-3 docosapentaenoic acid+docosahexaenoic acid, and the ratio of total omega-6 PUFA to total omega-3 PUFA in fingertip-prick blood, did not change from baseline during the postprandial period (P > .05). However, meal type yielded lower (P < .05) % omega-3 HUFA in total HUFA in the low fat meal (22.8 ± 3.9) as compared with the low fat with omega-3 (24.2 ± 3.9) and, the high fat (23.8 ± 4) meals. The ratio of total omega-6 PUFA to total omega-3 PUFA was generally higher in meals without omega-3 compared with omega-3. In conclusion, determinations of omega-3 status by fingertip-prick blood sampling may not require prior overnight fasting. SN - 1879-0739 UR - https://www.unboundmedicine.com/medline/citation/22935337/Omega_3_polyunsaturated_fatty_acid_profiling_using_fingertip_prick_whole_blood_does_not_require_overnight_fasting_before_blood_collection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0271-5317(12)00143-1 DB - PRIME DP - Unbound Medicine ER -