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Preparative isolation and purification of three stilbene glycosides from the tibetan medicinal plant Rheum tanguticum maxim. Ex Balf. by high-speed counter-current chromatography.
Phytochem Anal. 2013 Feb; 24(2):171-5.PA

Abstract

INTRODUCTION

Stilbene glycosides are the primary constituents of Rheum tanguticum Maxim. ex Balf., to which different bioactivities has been attributed, including: anti-HIV, anti-oxidant, anti-tumour, anti-malarial, and anti-allergy activity. However, effective methods for the isolation and purification of stilbene glycosides, such as trans-rhapontin, cis-rhapontin and trans-desoxyrhaponticin, from this herb are not currently available.

OBJECTIVE

To develop an efficient method for the preparative isolation and purification of three stilbene glycosides from Rheum tanguticum Maxim. ex Balf. via high-speed counter-current chromatography (HSCCC).

METHODS

A solvent system composed of chloroform:n-butanol:methanol:water (4:1:3:2, v/v/v/v) was developed for the separation. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase. The flow rate was 1.8 mL/min. The apparatus was controlled at 800 rpm and 25 °C, and the effluent was monitored at 280 nm. Chemical constituents were analysed by high-performance liquid chromatography (HPLC), and their structures were identified by ¹H- and ¹³C-NMR.

RESULTS

Under the optimised conditions, 25.5 mg trans-rhapontin, 16.0 mg cis-rhapontin and 20.5 mg trans-desoxyrhaponticin were separated from 80 mg crude sample; the isolates had purities of 99.6, 97.2 and 99.2%, respectively.

CONCLUSION

A simple and efficient HSCCC method has been optimised for the preparative separation of stilbene glycosides from Rheum tanguticum Maxim. ex Balf.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Zhao XH
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China. xhzhao@nwipb.ac.cn
Han F
No affiliation info available
Li YL
No affiliation info available
Yue HL
No affiliation info available

MeSH

Countercurrent DistributionGlycosidesMolecular StructurePlant RootsPlants, MedicinalReproducibility of ResultsRheumSolventsStilbenesTibet

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22936596

Citation

Zhao, Xiao-Hui, et al. "Preparative Isolation and Purification of Three Stilbene Glycosides From the Tibetan Medicinal Plant Rheum Tanguticum Maxim. Ex Balf. By High-speed Counter-current Chromatography." Phytochemical Analysis : PCA, vol. 24, no. 2, 2013, pp. 171-5.
Zhao XH, Han F, Li YL, et al. Preparative isolation and purification of three stilbene glycosides from the tibetan medicinal plant Rheum tanguticum maxim. Ex Balf. by high-speed counter-current chromatography. Phytochem Anal. 2013;24(2):171-5.
Zhao, X. H., Han, F., Li, Y. L., & Yue, H. L. (2013). Preparative isolation and purification of three stilbene glycosides from the tibetan medicinal plant Rheum tanguticum maxim. Ex Balf. by high-speed counter-current chromatography. Phytochemical Analysis : PCA, 24(2), 171-5. https://doi.org/10.1002/pca.2397
Zhao XH, et al. Preparative Isolation and Purification of Three Stilbene Glycosides From the Tibetan Medicinal Plant Rheum Tanguticum Maxim. Ex Balf. By High-speed Counter-current Chromatography. Phytochem Anal. 2013;24(2):171-5. PubMed PMID: 22936596.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparative isolation and purification of three stilbene glycosides from the tibetan medicinal plant Rheum tanguticum maxim. Ex Balf. by high-speed counter-current chromatography. AU - Zhao,Xiao-Hui, AU - Han,Fa, AU - Li,Yu-Lin, AU - Yue,Hui-Lan, Y1 - 2012/08/31/ PY - 2012/02/07/received PY - 2012/07/12/revised PY - 2012/07/17/accepted PY - 2012/9/1/entrez PY - 2012/9/1/pubmed PY - 2013/8/21/medline SP - 171 EP - 5 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 24 IS - 2 N2 - INTRODUCTION: Stilbene glycosides are the primary constituents of Rheum tanguticum Maxim. ex Balf., to which different bioactivities has been attributed, including: anti-HIV, anti-oxidant, anti-tumour, anti-malarial, and anti-allergy activity. However, effective methods for the isolation and purification of stilbene glycosides, such as trans-rhapontin, cis-rhapontin and trans-desoxyrhaponticin, from this herb are not currently available. OBJECTIVE: To develop an efficient method for the preparative isolation and purification of three stilbene glycosides from Rheum tanguticum Maxim. ex Balf. via high-speed counter-current chromatography (HSCCC). METHODS: A solvent system composed of chloroform:n-butanol:methanol:water (4:1:3:2, v/v/v/v) was developed for the separation. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase. The flow rate was 1.8 mL/min. The apparatus was controlled at 800 rpm and 25 °C, and the effluent was monitored at 280 nm. Chemical constituents were analysed by high-performance liquid chromatography (HPLC), and their structures were identified by ¹H- and ¹³C-NMR. RESULTS: Under the optimised conditions, 25.5 mg trans-rhapontin, 16.0 mg cis-rhapontin and 20.5 mg trans-desoxyrhaponticin were separated from 80 mg crude sample; the isolates had purities of 99.6, 97.2 and 99.2%, respectively. CONCLUSION: A simple and efficient HSCCC method has been optimised for the preparative separation of stilbene glycosides from Rheum tanguticum Maxim. ex Balf. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/22936596/Preparative_isolation_and_purification_of_three_stilbene_glycosides_from_the_tibetan_medicinal_plant_Rheum_tanguticum_maxim__Ex_Balf__by_high_speed_counter_current_chromatography_ L2 - https://doi.org/10.1002/pca.2397 DB - PRIME DP - Unbound Medicine ER -
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