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Label-free electrochemical aptasensor for sensitive thrombin detection using layer-by-layer self-assembled multilayers with toluidine blue-graphene composites and gold nanoparticles.
Talanta. 2012 Aug 30; 98:7-13.T

Abstract

In the present study, toluidine blue-graphene (Tb-Gra) nanocomposites were prepared to design a Lable-free electrochemical aptasensor for highly sensitive detection of thrombin based on layer-by-layer (LBL) technology. The nanocomposites with excellent redox electrochemical activities were first immobilized on the gold nanoparticles (nano-Au) modified glassy carbon electrodes (GCE). Then, the LBL structure was performed by electrostatic adsorption between the positively charged Tb-Gra and negatively charged nano-Au, which formed {Tb-Gra/nano-Au}(n) multilayer films for electroactive species enrichment and biomolecule immobilization. Subsequently, the thiolated thrombin binding aptamer (TBA) was assembled on the nano-Au surface through Au-S bond. In the presence of target thrombin (TB), the TBA on the multilayer could catch the thrombin onto the electrode surface, which resulted in a barrier for electro-transfer, leading to the decrease of the electrochemical signal of Tb-Gra nanocomposites. Under the optimal conditions, a wide detection range from 0.001 nM to 80 nM and a low detection limit of 0.33 pM (defined as S/N=3) for thrombin were obtained. In addition, the sensor exhibited excellent selectivity against other proteins.

Authors+Show Affiliations

Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22939121

Citation

Xie, Shunbi, et al. "Label-free Electrochemical Aptasensor for Sensitive Thrombin Detection Using Layer-by-layer Self-assembled Multilayers With Toluidine Blue-graphene Composites and Gold Nanoparticles." Talanta, vol. 98, 2012, pp. 7-13.
Xie S, Yuan R, Chai Y, et al. Label-free electrochemical aptasensor for sensitive thrombin detection using layer-by-layer self-assembled multilayers with toluidine blue-graphene composites and gold nanoparticles. Talanta. 2012;98:7-13.
Xie, S., Yuan, R., Chai, Y., Bai, L., Yuan, Y., & Wang, Y. (2012). Label-free electrochemical aptasensor for sensitive thrombin detection using layer-by-layer self-assembled multilayers with toluidine blue-graphene composites and gold nanoparticles. Talanta, 98, 7-13. https://doi.org/10.1016/j.talanta.2012.06.019
Xie S, et al. Label-free Electrochemical Aptasensor for Sensitive Thrombin Detection Using Layer-by-layer Self-assembled Multilayers With Toluidine Blue-graphene Composites and Gold Nanoparticles. Talanta. 2012 Aug 30;98:7-13. PubMed PMID: 22939121.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Label-free electrochemical aptasensor for sensitive thrombin detection using layer-by-layer self-assembled multilayers with toluidine blue-graphene composites and gold nanoparticles. AU - Xie,Shunbi, AU - Yuan,Ruo, AU - Chai,Yaqin, AU - Bai,Lijuan, AU - Yuan,Yali, AU - Wang,Yan, Y1 - 2012/06/22/ PY - 2012/03/07/received PY - 2012/06/06/revised PY - 2012/06/09/accepted PY - 2012/9/4/entrez PY - 2012/9/4/pubmed PY - 2013/1/18/medline SP - 7 EP - 13 JF - Talanta JO - Talanta VL - 98 N2 - In the present study, toluidine blue-graphene (Tb-Gra) nanocomposites were prepared to design a Lable-free electrochemical aptasensor for highly sensitive detection of thrombin based on layer-by-layer (LBL) technology. The nanocomposites with excellent redox electrochemical activities were first immobilized on the gold nanoparticles (nano-Au) modified glassy carbon electrodes (GCE). Then, the LBL structure was performed by electrostatic adsorption between the positively charged Tb-Gra and negatively charged nano-Au, which formed {Tb-Gra/nano-Au}(n) multilayer films for electroactive species enrichment and biomolecule immobilization. Subsequently, the thiolated thrombin binding aptamer (TBA) was assembled on the nano-Au surface through Au-S bond. In the presence of target thrombin (TB), the TBA on the multilayer could catch the thrombin onto the electrode surface, which resulted in a barrier for electro-transfer, leading to the decrease of the electrochemical signal of Tb-Gra nanocomposites. Under the optimal conditions, a wide detection range from 0.001 nM to 80 nM and a low detection limit of 0.33 pM (defined as S/N=3) for thrombin were obtained. In addition, the sensor exhibited excellent selectivity against other proteins. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/22939121/Label_free_electrochemical_aptasensor_for_sensitive_thrombin_detection_using_layer_by_layer_self_assembled_multilayers_with_toluidine_blue_graphene_composites_and_gold_nanoparticles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(12)00486-9 DB - PRIME DP - Unbound Medicine ER -