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Mechanisms of natural brassinosteroid-induced apoptosis of prostate cancer cells.
Food Chem Toxicol. 2012 Nov; 50(11):4068-76.FC

Abstract

Brassinosteroids (BRs) are a group of polyhydroxylated sterol derivatives with important regulatory roles in various plant physiological processes. The aim of this study was to examine the mechanism of the antiproliferative activity of natural BRs 28-homocastasterone (28-homoCS) and 24-epibrassinolide (24-epiBL) in hormone-sensitive and -insensitive (LNCaP and DU-145, respectively) human prostate cancer cell lines. The effects of BRs on prostate cancer cells were surveyed using flow cytometry, Western blotting, TUNEL, DNA ladder assays and immunofluorescence analyses. The studied BRs inhibited cell growth and induced G(1) blocks in LNCaP cells accompanied by reductions in cyclin D(1), CDK4/6 and pRb expression. Following BR treatment of DU-145 cells, increases in proportions of cells in the G(2)/M phase of cell cycle were observed, accompanied by down-regulation of cyclins A and B(1). Changes in AR localization patterns in LNCaP cells treated with BRs were shown by immunofluorescence analysis. Furthermore, apoptotic detection methods demonstrated induction of apoptosis mediated by BRs in both cell lines, although changes in the expression of apoptosis-related proteins were modulated differently by 28-homoCS and 24-piBL in each cell line. The studied BRs seem to exert potent growth inhibitory and pro-apoptotic effects and could be therefore highly valuable new candidates for prostate anticancer drugs.

Authors+Show Affiliations

Laboratory of Molecular Pathology, Department of Clinical and Molecular Pathology, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic. jana.steigerova@seznam.czNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22939933

Citation

Steigerová, Jana, et al. "Mechanisms of Natural Brassinosteroid-induced Apoptosis of Prostate Cancer Cells." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 50, no. 11, 2012, pp. 4068-76.
Steigerová J, Rárová L, Oklešt'ková J, et al. Mechanisms of natural brassinosteroid-induced apoptosis of prostate cancer cells. Food Chem Toxicol. 2012;50(11):4068-76.
Steigerová, J., Rárová, L., Oklešt'ková, J., Křížová, K., Levková, M., Sváchová, M., Kolář, Z., & Strnad, M. (2012). Mechanisms of natural brassinosteroid-induced apoptosis of prostate cancer cells. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 50(11), 4068-76. https://doi.org/10.1016/j.fct.2012.08.031
Steigerová J, et al. Mechanisms of Natural Brassinosteroid-induced Apoptosis of Prostate Cancer Cells. Food Chem Toxicol. 2012;50(11):4068-76. PubMed PMID: 22939933.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanisms of natural brassinosteroid-induced apoptosis of prostate cancer cells. AU - Steigerová,Jana, AU - Rárová,Lucie, AU - Oklešt'ková,Jana, AU - Křížová,Kateřina, AU - Levková,Monika, AU - Sváchová,Michaela, AU - Kolář,Zdeněk, AU - Strnad,Miroslav, Y1 - 2012/08/23/ PY - 2011/08/19/received PY - 2012/07/03/revised PY - 2012/08/15/accepted PY - 2012/9/4/entrez PY - 2012/9/4/pubmed PY - 2013/5/2/medline SP - 4068 EP - 76 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 50 IS - 11 N2 - Brassinosteroids (BRs) are a group of polyhydroxylated sterol derivatives with important regulatory roles in various plant physiological processes. The aim of this study was to examine the mechanism of the antiproliferative activity of natural BRs 28-homocastasterone (28-homoCS) and 24-epibrassinolide (24-epiBL) in hormone-sensitive and -insensitive (LNCaP and DU-145, respectively) human prostate cancer cell lines. The effects of BRs on prostate cancer cells were surveyed using flow cytometry, Western blotting, TUNEL, DNA ladder assays and immunofluorescence analyses. The studied BRs inhibited cell growth and induced G(1) blocks in LNCaP cells accompanied by reductions in cyclin D(1), CDK4/6 and pRb expression. Following BR treatment of DU-145 cells, increases in proportions of cells in the G(2)/M phase of cell cycle were observed, accompanied by down-regulation of cyclins A and B(1). Changes in AR localization patterns in LNCaP cells treated with BRs were shown by immunofluorescence analysis. Furthermore, apoptotic detection methods demonstrated induction of apoptosis mediated by BRs in both cell lines, although changes in the expression of apoptosis-related proteins were modulated differently by 28-homoCS and 24-piBL in each cell line. The studied BRs seem to exert potent growth inhibitory and pro-apoptotic effects and could be therefore highly valuable new candidates for prostate anticancer drugs. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/22939933/Mechanisms_of_natural_brassinosteroid_induced_apoptosis_of_prostate_cancer_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(12)00611-4 DB - PRIME DP - Unbound Medicine ER -