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Characterization of the procera tomato mutant shows novel functions of the SlDELLA protein in the control of flower morphology, cell division and expansion, and the auxin-signaling pathway during fruit-set and development.
Plant Physiol. 2012 Nov; 160(3):1581-96.PP

Abstract

procera (pro) is a tall tomato (Solanum lycopersicum) mutant carrying a point mutation in the GRAS region of the gene encoding SlDELLA, a repressor in the gibberellin (GA) signaling pathway. Consistent with the SlDELLA loss of function, pro plants display a GA-constitutive response phenotype, mimicking wild-type plants treated with GA₃. The ovaries from both nonemasculated and emasculated pro flowers had very strong parthenocarpic capacity, associated with enhanced growth of preanthesis ovaries due to more and larger cells. pro parthenocarpy is facultative because seeded fruits were obtained by manual pollination. Most pro pistils had exserted stigmas, thus preventing self-pollination, similar to wild-type pistils treated with GA₃ or auxins. However, Style2.1, a gene responsible for long styles in noncultivated tomato, may not control the enhanced style elongation of pro pistils, because its expression was not higher in pro styles and did not increase upon GA₃ application. Interestingly, a high percentage of pro flowers had meristic alterations, with one additional petal, sepal, stamen, and carpel at each of the four whorls, respectively, thus unveiling a role of SlDELLA in flower organ development. Microarray analysis showed significant changes in the transcriptome of preanthesis pro ovaries compared with the wild type, indicating that the molecular mechanism underlying the parthenocarpic capacity of pro is complex and that it is mainly associated with changes in the expression of genes involved in GA and auxin pathways. Interestingly, it was found that GA activity modulates the expression of cell division and expansion genes and an auxin signaling gene (tomato AUXIN RESPONSE FACTOR7) during fruit-set.

Authors+Show Affiliations

Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, Ingeniero Fausto Elio s/n, 46022 Valencia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22942390

Citation

Carrera, Esther, et al. "Characterization of the Procera Tomato Mutant Shows Novel Functions of the SlDELLA Protein in the Control of Flower Morphology, Cell Division and Expansion, and the Auxin-signaling Pathway During Fruit-set and Development." Plant Physiology, vol. 160, no. 3, 2012, pp. 1581-96.
Carrera E, Ruiz-Rivero O, Peres LE, et al. Characterization of the procera tomato mutant shows novel functions of the SlDELLA protein in the control of flower morphology, cell division and expansion, and the auxin-signaling pathway during fruit-set and development. Plant Physiol. 2012;160(3):1581-96.
Carrera, E., Ruiz-Rivero, O., Peres, L. E., Atares, A., & Garcia-Martinez, J. L. (2012). Characterization of the procera tomato mutant shows novel functions of the SlDELLA protein in the control of flower morphology, cell division and expansion, and the auxin-signaling pathway during fruit-set and development. Plant Physiology, 160(3), 1581-96. https://doi.org/10.1104/pp.112.204552
Carrera E, et al. Characterization of the Procera Tomato Mutant Shows Novel Functions of the SlDELLA Protein in the Control of Flower Morphology, Cell Division and Expansion, and the Auxin-signaling Pathway During Fruit-set and Development. Plant Physiol. 2012;160(3):1581-96. PubMed PMID: 22942390.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the procera tomato mutant shows novel functions of the SlDELLA protein in the control of flower morphology, cell division and expansion, and the auxin-signaling pathway during fruit-set and development. AU - Carrera,Esther, AU - Ruiz-Rivero,Omar, AU - Peres,Lazaro Eustaquio Pereira, AU - Atares,Alejandro, AU - Garcia-Martinez,Jose Luis, Y1 - 2012/08/31/ PY - 2012/9/4/entrez PY - 2012/9/4/pubmed PY - 2013/4/23/medline SP - 1581 EP - 96 JF - Plant physiology JO - Plant Physiol VL - 160 IS - 3 N2 - procera (pro) is a tall tomato (Solanum lycopersicum) mutant carrying a point mutation in the GRAS region of the gene encoding SlDELLA, a repressor in the gibberellin (GA) signaling pathway. Consistent with the SlDELLA loss of function, pro plants display a GA-constitutive response phenotype, mimicking wild-type plants treated with GA₃. The ovaries from both nonemasculated and emasculated pro flowers had very strong parthenocarpic capacity, associated with enhanced growth of preanthesis ovaries due to more and larger cells. pro parthenocarpy is facultative because seeded fruits were obtained by manual pollination. Most pro pistils had exserted stigmas, thus preventing self-pollination, similar to wild-type pistils treated with GA₃ or auxins. However, Style2.1, a gene responsible for long styles in noncultivated tomato, may not control the enhanced style elongation of pro pistils, because its expression was not higher in pro styles and did not increase upon GA₃ application. Interestingly, a high percentage of pro flowers had meristic alterations, with one additional petal, sepal, stamen, and carpel at each of the four whorls, respectively, thus unveiling a role of SlDELLA in flower organ development. Microarray analysis showed significant changes in the transcriptome of preanthesis pro ovaries compared with the wild type, indicating that the molecular mechanism underlying the parthenocarpic capacity of pro is complex and that it is mainly associated with changes in the expression of genes involved in GA and auxin pathways. Interestingly, it was found that GA activity modulates the expression of cell division and expansion genes and an auxin signaling gene (tomato AUXIN RESPONSE FACTOR7) during fruit-set. SN - 1532-2548 UR - https://www.unboundmedicine.com/medline/citation/22942390/Characterization_of_the_procera_tomato_mutant_shows_novel_functions_of_the_SlDELLA_protein_in_the_control_of_flower_morphology_cell_division_and_expansion_and_the_auxin_signaling_pathway_during_fruit_set_and_development_ L2 - http://www.plantphysiol.org/cgi/pmidlookup?view=long&pmid=22942390 DB - PRIME DP - Unbound Medicine ER -