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Ultrasensitive and selective homogeneous sandwich immunoassay detection by Surface Enhanced Raman Scattering (SERS).
Analyst. 2012 Oct 21; 137(20):4834-40.A

Abstract

In this report, a simple and highly selective homogeneous sandwich immunoassay was developed for ultrasensitive detection of Staphylococcal Enterotoxin B (SEB) using Surface Enhanced Raman Scattering (SERS). The assay uses polyclonal-antibody functionalized magnetic gold nanorod particles as capture probes for SEB, which can be collected via a simple magnet. After separating SEB from the sample matrix, they are sandwiched by using binding-specific antibody-antigen pairs with the help of gold nanorod particles. Gold nanorod particles are bifunctional by design and contain self-assembled monolayers (SAMs) of a SERS tag molecule (5,5-dithiobis (2-nitrobenzoic acid), DTNB) and carboxylic functionalities of DTNB for coupling with a suitable antibody. The correlation between the SEB concentration and SERS signal was found to be linear within the range of 3 fM to 0.3 μM. The limit of detection for the assay was determined to be 768 aM (ca., 9250 SEB molecules per 20 μL sample volume). The gold heterogeneous assay system for SEB detection was also compared with the same SERS probes and gold-coated surfaces as capture substrates. The developed method was further evaluated for detecting SEB in artificially contaminated milk. Finally, the method was used for investigating the SEB specificity on bovine serum albumin (BSA) and avidin.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Etiler, Ankara, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22943047

Citation

Pekdemir, Mustafa Ersin, et al. "Ultrasensitive and Selective Homogeneous Sandwich Immunoassay Detection By Surface Enhanced Raman Scattering (SERS)." The Analyst, vol. 137, no. 20, 2012, pp. 4834-40.
Pekdemir ME, Ertürkan D, Külah H, et al. Ultrasensitive and selective homogeneous sandwich immunoassay detection by Surface Enhanced Raman Scattering (SERS). Analyst. 2012;137(20):4834-40.
Pekdemir, M. E., Ertürkan, D., Külah, H., Boyacı, I. H., Ozgen, C., & Tamer, U. (2012). Ultrasensitive and selective homogeneous sandwich immunoassay detection by Surface Enhanced Raman Scattering (SERS). The Analyst, 137(20), 4834-40. https://doi.org/10.1039/c2an35471c
Pekdemir ME, et al. Ultrasensitive and Selective Homogeneous Sandwich Immunoassay Detection By Surface Enhanced Raman Scattering (SERS). Analyst. 2012 Oct 21;137(20):4834-40. PubMed PMID: 22943047.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrasensitive and selective homogeneous sandwich immunoassay detection by Surface Enhanced Raman Scattering (SERS). AU - Pekdemir,Mustafa Ersin, AU - Ertürkan,Deniz, AU - Külah,Haluk, AU - Boyacı,Ismail H, AU - Ozgen,Canan, AU - Tamer,Uğur, PY - 2012/9/4/entrez PY - 2012/9/4/pubmed PY - 2012/9/4/medline SP - 4834 EP - 40 JF - The Analyst JO - Analyst VL - 137 IS - 20 N2 - In this report, a simple and highly selective homogeneous sandwich immunoassay was developed for ultrasensitive detection of Staphylococcal Enterotoxin B (SEB) using Surface Enhanced Raman Scattering (SERS). The assay uses polyclonal-antibody functionalized magnetic gold nanorod particles as capture probes for SEB, which can be collected via a simple magnet. After separating SEB from the sample matrix, they are sandwiched by using binding-specific antibody-antigen pairs with the help of gold nanorod particles. Gold nanorod particles are bifunctional by design and contain self-assembled monolayers (SAMs) of a SERS tag molecule (5,5-dithiobis (2-nitrobenzoic acid), DTNB) and carboxylic functionalities of DTNB for coupling with a suitable antibody. The correlation between the SEB concentration and SERS signal was found to be linear within the range of 3 fM to 0.3 μM. The limit of detection for the assay was determined to be 768 aM (ca., 9250 SEB molecules per 20 μL sample volume). The gold heterogeneous assay system for SEB detection was also compared with the same SERS probes and gold-coated surfaces as capture substrates. The developed method was further evaluated for detecting SEB in artificially contaminated milk. Finally, the method was used for investigating the SEB specificity on bovine serum albumin (BSA) and avidin. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/22943047/Ultrasensitive_and_selective_homogeneous_sandwich_immunoassay_detection_by_Surface_Enhanced_Raman_Scattering__SERS__ L2 - https://doi.org/10.1039/c2an35471c DB - PRIME DP - Unbound Medicine ER -
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