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Inhibitory effects of Zataria multiflora essential oil and its main components on nitric oxide and hydrogen peroxide production in lipopolysaccharide-stimulated macrophages.
J Pharm Pharmacol. 2012 Oct; 64(10):1491-500.JP

Abstract

OBJECTIVES

Zataria multiflora is an aromatic plant that is used in flavouring and preserving foods and also used as an antispasmodic, anaesthetic and antinociceptive agent. In this study, the effects of Z. multiflora essential oil on nitric oxide (NO) and hydrogen peroxide (H(2) O(2)) production in lipopolysaccharide (LPS)-stimulated macrophages was investigated.

METHODS

Z. multiflora essential oil was extracted by water-distillation, analysed by GC-MS and then the effect of the essential oil on NO and H(2) O(2) production was investigated.

KEY FINDINGS

Carvacrol (52%), thymol (16%) and p-cymene (10%) were the main components of the oil. The IC50 (concentration providing 50% inhibition) for reactive oxygen scavenging was estimated to be 5.7, 3 and 4.2 µg/ml for the essential oil, thymol and carvacrol, respectively, while the corresponding IC50 values for reactive nitrogen scavenging were estimated to be 8.6, 4.7 and 6.6 µg/ml. Z. multiflora essential oil, thymol, and carvacrol significantly reduced NO and H(2) O(2) production as well as NO synthase and NADH oxidase activity in LPS-stimulated murine macrophages while p-cymene did not show any antioxidant activity.

CONCLUSIONS

Z. multiflora essential oil has the potential to be used in the therapy of oxidative damage.

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Authors+Show Affiliations

Kavoosi G
Institute of Biotechnology Department of Horticultural Sciences, Faculty of Agriculture, Shiraz University, Shiraz, Iran. ghkavoosi@shirazu.ac.ir
Teixeira da Silva JA
No affiliation info available
Saharkhiz MJ
No affiliation info available

MeSH

AnimalsAntioxidantsCell LineGas Chromatography-Mass SpectrometryHydrogen PeroxideInhibitory Concentration 50LamiaceaeLipopolysaccharidesMacrophagesMiceMultienzyme ComplexesNADH, NADPH OxidoreductasesNitric OxideNitric Oxide SynthaseOils, VolatileOxidative StressPlant ExtractsReactive Nitrogen SpeciesReactive Oxygen Species

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22943180

Citation

Kavoosi, Gholamreza, et al. "Inhibitory Effects of Zataria Multiflora Essential Oil and Its Main Components On Nitric Oxide and Hydrogen Peroxide Production in Lipopolysaccharide-stimulated Macrophages." The Journal of Pharmacy and Pharmacology, vol. 64, no. 10, 2012, pp. 1491-500.
Kavoosi G, Teixeira da Silva JA, Saharkhiz MJ. Inhibitory effects of Zataria multiflora essential oil and its main components on nitric oxide and hydrogen peroxide production in lipopolysaccharide-stimulated macrophages. J Pharm Pharmacol. 2012;64(10):1491-500.
Kavoosi, G., Teixeira da Silva, J. A., & Saharkhiz, M. J. (2012). Inhibitory effects of Zataria multiflora essential oil and its main components on nitric oxide and hydrogen peroxide production in lipopolysaccharide-stimulated macrophages. The Journal of Pharmacy and Pharmacology, 64(10), 1491-500. https://doi.org/10.1111/j.2042-7158.2012.01510.x
Kavoosi G, Teixeira da Silva JA, Saharkhiz MJ. Inhibitory Effects of Zataria Multiflora Essential Oil and Its Main Components On Nitric Oxide and Hydrogen Peroxide Production in Lipopolysaccharide-stimulated Macrophages. J Pharm Pharmacol. 2012;64(10):1491-500. PubMed PMID: 22943180.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effects of Zataria multiflora essential oil and its main components on nitric oxide and hydrogen peroxide production in lipopolysaccharide-stimulated macrophages. AU - Kavoosi,Gholamreza, AU - Teixeira da Silva,Jaime A, AU - Saharkhiz,Mohammad J, Y1 - 2012/04/05/ PY - 2012/9/5/entrez PY - 2012/9/5/pubmed PY - 2013/2/5/medline SP - 1491 EP - 500 JF - The Journal of pharmacy and pharmacology JO - J Pharm Pharmacol VL - 64 IS - 10 N2 - OBJECTIVES: Zataria multiflora is an aromatic plant that is used in flavouring and preserving foods and also used as an antispasmodic, anaesthetic and antinociceptive agent. In this study, the effects of Z. multiflora essential oil on nitric oxide (NO) and hydrogen peroxide (H(2) O(2)) production in lipopolysaccharide (LPS)-stimulated macrophages was investigated. METHODS: Z. multiflora essential oil was extracted by water-distillation, analysed by GC-MS and then the effect of the essential oil on NO and H(2) O(2) production was investigated. KEY FINDINGS: Carvacrol (52%), thymol (16%) and p-cymene (10%) were the main components of the oil. The IC50 (concentration providing 50% inhibition) for reactive oxygen scavenging was estimated to be 5.7, 3 and 4.2 µg/ml for the essential oil, thymol and carvacrol, respectively, while the corresponding IC50 values for reactive nitrogen scavenging were estimated to be 8.6, 4.7 and 6.6 µg/ml. Z. multiflora essential oil, thymol, and carvacrol significantly reduced NO and H(2) O(2) production as well as NO synthase and NADH oxidase activity in LPS-stimulated murine macrophages while p-cymene did not show any antioxidant activity. CONCLUSIONS: Z. multiflora essential oil has the potential to be used in the therapy of oxidative damage. SN - 2042-7158 UR - https://www.unboundmedicine.com/medline/citation/22943180/Inhibitory_effects_of_Zataria_multiflora_essential_oil_and_its_main_components_on_nitric_oxide_and_hydrogen_peroxide_production_in_lipopolysaccharide_stimulated_macrophages_ L2 - https://doi.org/10.1111/j.2042-7158.2012.01510.x DB - PRIME DP - Unbound Medicine ER -