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Potentiation of etoposide and temozolomide cytotoxicity by curcumin and turmeric force™ in brain tumor cell lines.

Abstract

We have investigated on the potentiation of etoposide (ETP) and temozolomide (TMZ) cytotoxicity in U-87MG glioblastoma and D283 medulloblastoma cell lines by curcumin (CUR) and turmeric force (TF), a nutraceutical formulation of turmeric, with the objective of assessing the potential for their adjuvant use in brain tumor chemotherapy. While U-87MG cell line was generally resistant to TMZ, IC50 values for CUR and TF were 37.33 and 30.75 µg/ml, respectively. TF is the only agent that demonstrated efficacy at the IC90 level. When CUR or TF was combined with ETP and TMZ, increased chemotherapeutic efficiency in the U-87MG cells was observed. TF is highly cytotoxic to D283 Med cell line compared to curcumin with an IC50 value of 1.55 ug/ml. Although both CUR and TF potentiated ETP and TMZ cytotoxicity, TF is more efficient than CUR in both U-87MG and D283 Med cell lines. Treatment of U-87MG cells with the triple combination of TMZ+ETP+TF induced a high percentage of apoptotic cells. Potential mechanisms that may explain evidence of synergy include down regulation of p10 and p53 mRNAs and increase in BAX/Bcl-2 mRNA ratio. These pre-clinical results suggest that TF may be useful as an adjuvant with ETP and TMZ for brain tumor chemotherapy.

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    MeSH

    Antineoplastic Agents
    Antineoplastic Agents, Alkylating
    Antineoplastic Agents, Phytogenic
    Antineoplastic Combined Chemotherapy Protocols
    Apoptosis
    Brain Neoplasms
    Cell Line, Tumor
    Cell Proliferation
    Cerebellar Neoplasms
    Curcuma
    Curcumin
    Dacarbazine
    Dose-Response Relationship, Drug
    Drug Synergism
    Etoposide
    Glioblastoma
    Humans
    Inhibitory Concentration 50
    Medulloblastoma
    Phytotherapy
    Plant Extracts
    Plants, Medicinal
    Rhizome

    Pub Type(s)

    Evaluation Studies
    Journal Article

    Language

    eng

    PubMed ID

    22944718

    Citation

    TY - JOUR T1 - Potentiation of etoposide and temozolomide cytotoxicity by curcumin and turmeric force™ in brain tumor cell lines. AU - Ramachandran,Cheppail, AU - Nair,Smitha M, AU - Escalon,Enrique, AU - Melnick,Steven J, Y1 - 2012/08/10/ PY - 2012/9/5/entrez PY - 2012/9/5/pubmed PY - 2013/1/23/medline SP - Article 20 EP - Article 20 JF - Journal of complementary & integrative medicine JO - J Complement Integr Med VL - 9 N2 - We have investigated on the potentiation of etoposide (ETP) and temozolomide (TMZ) cytotoxicity in U-87MG glioblastoma and D283 medulloblastoma cell lines by curcumin (CUR) and turmeric force (TF), a nutraceutical formulation of turmeric, with the objective of assessing the potential for their adjuvant use in brain tumor chemotherapy. While U-87MG cell line was generally resistant to TMZ, IC50 values for CUR and TF were 37.33 and 30.75 µg/ml, respectively. TF is the only agent that demonstrated efficacy at the IC90 level. When CUR or TF was combined with ETP and TMZ, increased chemotherapeutic efficiency in the U-87MG cells was observed. TF is highly cytotoxic to D283 Med cell line compared to curcumin with an IC50 value of 1.55 ug/ml. Although both CUR and TF potentiated ETP and TMZ cytotoxicity, TF is more efficient than CUR in both U-87MG and D283 Med cell lines. Treatment of U-87MG cells with the triple combination of TMZ+ETP+TF induced a high percentage of apoptotic cells. Potential mechanisms that may explain evidence of synergy include down regulation of p10 and p53 mRNAs and increase in BAX/Bcl-2 mRNA ratio. These pre-clinical results suggest that TF may be useful as an adjuvant with ETP and TMZ for brain tumor chemotherapy. SN - 1553-3840 UR - https://www.unboundmedicine.com/medline/citation/22944718/Potentiation_of_etoposide_and_temozolomide_cytotoxicity_by_curcumin_and_turmeric_force™_in_brain_tumor_cell_lines_ L2 - http://www.degruyter.com/doi/10.1515/1553-3840.1614 ER -