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Potentiation of etoposide and temozolomide cytotoxicity by curcumin and turmeric force™ in brain tumor cell lines.

Abstract

We have investigated on the potentiation of etoposide (ETP) and temozolomide (TMZ) cytotoxicity in U-87MG glioblastoma and D283 medulloblastoma cell lines by curcumin (CUR) and turmeric force (TF), a nutraceutical formulation of turmeric, with the objective of assessing the potential for their adjuvant use in brain tumor chemotherapy. While U-87MG cell line was generally resistant to TMZ, IC50 values for CUR and TF were 37.33 and 30.75 µg/ml, respectively. TF is the only agent that demonstrated efficacy at the IC90 level. When CUR or TF was combined with ETP and TMZ, increased chemotherapeutic efficiency in the U-87MG cells was observed. TF is highly cytotoxic to D283 Med cell line compared to curcumin with an IC50 value of 1.55 ug/ml. Although both CUR and TF potentiated ETP and TMZ cytotoxicity, TF is more efficient than CUR in both U-87MG and D283 Med cell lines. Treatment of U-87MG cells with the triple combination of TMZ+ETP+TF induced a high percentage of apoptotic cells. Potential mechanisms that may explain evidence of synergy include down regulation of p10 and p53 mRNAs and increase in BAX/Bcl-2 mRNA ratio. These pre-clinical results suggest that TF may be useful as an adjuvant with ETP and TMZ for brain tumor chemotherapy.

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    MeSH

    Antineoplastic Agents
    Antineoplastic Agents, Alkylating
    Antineoplastic Agents, Phytogenic
    Antineoplastic Combined Chemotherapy Protocols
    Apoptosis
    Brain Neoplasms
    Cell Line, Tumor
    Cell Proliferation
    Cerebellar Neoplasms
    Curcuma
    Curcumin
    Dacarbazine
    Dose-Response Relationship, Drug
    Drug Synergism
    Etoposide
    Glioblastoma
    Humans
    Inhibitory Concentration 50
    Medulloblastoma
    Phytotherapy
    Plant Extracts
    Plants, Medicinal
    Rhizome

    Pub Type(s)

    Evaluation Studies
    Journal Article

    Language

    eng

    PubMed ID

    22944718