Tags

Type your tag names separated by a space and hit enter

First Korean case of Robinsoniella peoriensis bacteremia in a patient with aspiration pneumonia.
Ann Lab Med. 2012 Sep; 32(5):370-4.AL

Abstract

Robinsoniella peoriensis has recently been identified as a Gram-positive, spore-forming, anaerobic rod originally recovered from swine manure storage pits. To date, 6 cases of R. peoriensis infection have been reported, including 2 cases of bacteremia, 1 of abdominal fluid collection, and 3 of wound infection. In the present study, we report a 76-yr-old man with R. peoriensis bacteremia who developed aspiration pneumonia. Gram staining of a purified colony revealed Gram-positive, rod-shaped bacteria. Biochemical identification using API 20 A (bioMérieux, France) indicated presence of Clostridium spp. We performed both 500-bp and full-gene sequencing of 16S rRNA of the isolate. The sequence was analyzed with MicroSeq ID 16S rRNA Library v2.0 (Applied Biosystems, USA), GenBank Basic Local Alignment Search Tool (BLAST) (http://www.ncbi.nlm.nih.gov/genbank), and EzTaxon database v2.1 (http://www.eztaxon.org). The 500-bp 16S rRNA sequence of the blood culture isolate showed 99.16-99.79% similarity with R. peoriensis and the full-gene 16S rRNA sequence showed 98.87-99.50% similarity with R. peoriensis. The organism was confirmed as R. peoriensis by using all of the mentioned databases except for MicroSeq, which did not include the RNA sequence of this bacterium. This case suggests that identification of R. peoriensis might be challenging in clinical laboratories with no access to molecular methods, as certain commercial identification systems may not identify, or may misidentify, this organism. To the best of our knowledge, this is the first report of the isolation of R. peoriensis in Korea.

Authors+Show Affiliations

Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

22950075

Citation

Jeon, Yongbum, et al. "First Korean Case of Robinsoniella Peoriensis Bacteremia in a Patient With Aspiration Pneumonia." Annals of Laboratory Medicine, vol. 32, no. 5, 2012, pp. 370-4.
Jeon Y, Kim TS, Kim HB, et al. First Korean case of Robinsoniella peoriensis bacteremia in a patient with aspiration pneumonia. Ann Lab Med. 2012;32(5):370-4.
Jeon, Y., Kim, T. S., Kim, H. B., Park, K. U., Song, J., & Kim, E. C. (2012). First Korean case of Robinsoniella peoriensis bacteremia in a patient with aspiration pneumonia. Annals of Laboratory Medicine, 32(5), 370-4. https://doi.org/10.3343/alm.2012.32.5.370
Jeon Y, et al. First Korean Case of Robinsoniella Peoriensis Bacteremia in a Patient With Aspiration Pneumonia. Ann Lab Med. 2012;32(5):370-4. PubMed PMID: 22950075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - First Korean case of Robinsoniella peoriensis bacteremia in a patient with aspiration pneumonia. AU - Jeon,Yongbum, AU - Kim,Taek Soo, AU - Kim,Hong Bin, AU - Park,Kyoung Un, AU - Song,Junghan, AU - Kim,Eui Chong, Y1 - 2012/08/13/ PY - 2012/04/09/received PY - 2012/04/28/revised PY - 2012/07/17/accepted PY - 2012/9/6/entrez PY - 2012/9/6/pubmed PY - 2013/1/12/medline KW - 16S ribosomal RNA KW - Bacteremia KW - Robinsoniella peoriensis SP - 370 EP - 4 JF - Annals of laboratory medicine JO - Ann Lab Med VL - 32 IS - 5 N2 - Robinsoniella peoriensis has recently been identified as a Gram-positive, spore-forming, anaerobic rod originally recovered from swine manure storage pits. To date, 6 cases of R. peoriensis infection have been reported, including 2 cases of bacteremia, 1 of abdominal fluid collection, and 3 of wound infection. In the present study, we report a 76-yr-old man with R. peoriensis bacteremia who developed aspiration pneumonia. Gram staining of a purified colony revealed Gram-positive, rod-shaped bacteria. Biochemical identification using API 20 A (bioMérieux, France) indicated presence of Clostridium spp. We performed both 500-bp and full-gene sequencing of 16S rRNA of the isolate. The sequence was analyzed with MicroSeq ID 16S rRNA Library v2.0 (Applied Biosystems, USA), GenBank Basic Local Alignment Search Tool (BLAST) (http://www.ncbi.nlm.nih.gov/genbank), and EzTaxon database v2.1 (http://www.eztaxon.org). The 500-bp 16S rRNA sequence of the blood culture isolate showed 99.16-99.79% similarity with R. peoriensis and the full-gene 16S rRNA sequence showed 98.87-99.50% similarity with R. peoriensis. The organism was confirmed as R. peoriensis by using all of the mentioned databases except for MicroSeq, which did not include the RNA sequence of this bacterium. This case suggests that identification of R. peoriensis might be challenging in clinical laboratories with no access to molecular methods, as certain commercial identification systems may not identify, or may misidentify, this organism. To the best of our knowledge, this is the first report of the isolation of R. peoriensis in Korea. SN - 2234-3814 UR - https://www.unboundmedicine.com/medline/citation/22950075/First_Korean_case_of_Robinsoniella_peoriensis_bacteremia_in_a_patient_with_aspiration_pneumonia_ L2 - http://www.annlabmed.org/journal/viewJournal.html?year=2012&vol=32&page=370 DB - PRIME DP - Unbound Medicine ER -