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Deconvolution of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry isotope patterns to determine ratios of A-type to B-type interflavan bonds in cranberry proanthocyanidins.
Food Chem 2012; 135(3):1485-93FC

Abstract

A method to deconvolute overlapping isotope patterns in positive mode matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was developed to determine ratios of A- to B-type interflavan bonds in proanthocyanidins that were isolated from cranberry (Vaccinium macrocarpon, Ait.) press cake (c-PAC). Precision and accuracy was validated for binary mixtures of procyanidins A2 and B2. Deconvolution of c-PAC spectra indicated that oligomers with one or more A-type interflavan bonds occur in a higher proportion than oligomers with all B-type interflavan bonds. c-PAC with at least one A-type bond accounted for more than 91% of the oligomers between trimers and undecamers. The c-PAC isotope patterns are highly repeatable, suggesting that the method can be applied to authentication, standardization and efficacy of cranberry products in relationship to urinary tract health. This is the first time MALDI-TOF MS has been used for estimating ratios of A- to B-type bonds in PAC.

Authors+Show Affiliations

University of Wisconsin-Madison, Dept. of Food Science, 1605 Linden Drive, Madison, WI 53706, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22953884

Citation

Feliciano, Rodrigo P., et al. "Deconvolution of Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry Isotope Patterns to Determine Ratios of A-type to B-type Interflavan Bonds in Cranberry Proanthocyanidins." Food Chemistry, vol. 135, no. 3, 2012, pp. 1485-93.
Feliciano RP, Krueger CG, Shanmuganayagam D, et al. Deconvolution of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry isotope patterns to determine ratios of A-type to B-type interflavan bonds in cranberry proanthocyanidins. Food Chem. 2012;135(3):1485-93.
Feliciano, R. P., Krueger, C. G., Shanmuganayagam, D., Vestling, M. M., & Reed, J. D. (2012). Deconvolution of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry isotope patterns to determine ratios of A-type to B-type interflavan bonds in cranberry proanthocyanidins. Food Chemistry, 135(3), pp. 1485-93. doi:10.1016/j.foodchem.2012.05.102.
Feliciano RP, et al. Deconvolution of Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry Isotope Patterns to Determine Ratios of A-type to B-type Interflavan Bonds in Cranberry Proanthocyanidins. Food Chem. 2012 Dec 1;135(3):1485-93. PubMed PMID: 22953884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deconvolution of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry isotope patterns to determine ratios of A-type to B-type interflavan bonds in cranberry proanthocyanidins. AU - Feliciano,Rodrigo P, AU - Krueger,Christian G, AU - Shanmuganayagam,Dhanansayan, AU - Vestling,Martha M, AU - Reed,Jess D, Y1 - 2012/06/07/ PY - 2011/12/13/received PY - 2012/05/04/revised PY - 2012/05/28/accepted PY - 2012/9/8/entrez PY - 2012/9/8/pubmed PY - 2013/2/7/medline SP - 1485 EP - 93 JF - Food chemistry JO - Food Chem VL - 135 IS - 3 N2 - A method to deconvolute overlapping isotope patterns in positive mode matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was developed to determine ratios of A- to B-type interflavan bonds in proanthocyanidins that were isolated from cranberry (Vaccinium macrocarpon, Ait.) press cake (c-PAC). Precision and accuracy was validated for binary mixtures of procyanidins A2 and B2. Deconvolution of c-PAC spectra indicated that oligomers with one or more A-type interflavan bonds occur in a higher proportion than oligomers with all B-type interflavan bonds. c-PAC with at least one A-type bond accounted for more than 91% of the oligomers between trimers and undecamers. The c-PAC isotope patterns are highly repeatable, suggesting that the method can be applied to authentication, standardization and efficacy of cranberry products in relationship to urinary tract health. This is the first time MALDI-TOF MS has been used for estimating ratios of A- to B-type bonds in PAC. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/22953884/Deconvolution_of_matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_isotope_patterns_to_determine_ratios_of_A_type_to_B_type_interflavan_bonds_in_cranberry_proanthocyanidins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(12)00951-X DB - PRIME DP - Unbound Medicine ER -