Tags

Type your tag names separated by a space and hit enter

Anti-diabetic effects of Centratherum anthelminticum seeds methanolic fraction on pancreatic cells, β-TC6 and its alleviating role in type 2 diabetic rats.
J Ethnopharmacol. 2012 Oct 31; 144(1):22-32.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Seeds of Centratherum anthelminticum (Asteraceae) have been popularly used in Ayurvedic medicine to treat diabetes and skin disorders. Folk medicine from Rayalaseema (Andhra Pradesh, India) reported wide spread usage in diabetes.

AIM OF THE STUDY

To investigate the hypoglycemic properties and mechanism of the methanolic fraction of C. anthelminticum seeds (CAMFs) on mouse β-TC6 pancreatic cell line and streptozotocin (STZ)-induced diabetic rat models.

MATERIALS AND METHODS

We investigated the crude methanolic fraction of C. anthelminticum seeds (CAMFs) on β-TC6 cell line and confirmed its effects on type 1 and type 2 diabetic rats to understand its mechanism in managing diabetes mellitus. CAMFs were initially tested on β-TC6 cells for cytotoxicity, 2-NBDG glucose uptake, insulin secretion and glucose transporter (GLUT-1, 2 and 4) protein expression. Furthermore, streptozotocin (STZ)-induced type 1 diabetic and STZ-nicotinamide-induced type 2 diabetic rats were intraperitoneally (i.p) injected or administered orally with CAMFs daily for 28 days. The effect of CAMFs on blood glucose and insulin levels was subsequently evaluated.

RESULTS

In cell line studies, CAMFs showed non-cytotoxic effect on β-TC6 cell proliferation compared to untreated control cells at 50 μg/ml. CAMFs increased glucose uptake and insulin secretion dose-dependently by up-regulating GLUT-2 and GLUT-4 expression in these cells. Further in vivo studies on streptozotocin induced diabetic rat models revealed that CAMFs significantly reduced hyperglycemia by augmenting insulin secretion in type 2 diabetic rats. However, CAMFs displayed less significant effects on type 1 diabetic rats.

CONCLUSIONS

CAMFs demonstrated anti-diabetic potential on β-TC6 cells and type 2 diabetic rat model, plausibly through enhancing glucose uptake and insulin secretion.

Authors+Show Affiliations

Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22954496

Citation

Arya, Aditya, et al. "Anti-diabetic Effects of Centratherum Anthelminticum Seeds Methanolic Fraction On Pancreatic Cells, β-TC6 and Its Alleviating Role in Type 2 Diabetic Rats." Journal of Ethnopharmacology, vol. 144, no. 1, 2012, pp. 22-32.
Arya A, Looi CY, Cheah SC, et al. Anti-diabetic effects of Centratherum anthelminticum seeds methanolic fraction on pancreatic cells, β-TC6 and its alleviating role in type 2 diabetic rats. J Ethnopharmacol. 2012;144(1):22-32.
Arya, A., Looi, C. Y., Cheah, S. C., Mustafa, M. R., & Mohd, M. A. (2012). Anti-diabetic effects of Centratherum anthelminticum seeds methanolic fraction on pancreatic cells, β-TC6 and its alleviating role in type 2 diabetic rats. Journal of Ethnopharmacology, 144(1), 22-32. https://doi.org/10.1016/j.jep.2012.08.014
Arya A, et al. Anti-diabetic Effects of Centratherum Anthelminticum Seeds Methanolic Fraction On Pancreatic Cells, β-TC6 and Its Alleviating Role in Type 2 Diabetic Rats. J Ethnopharmacol. 2012 Oct 31;144(1):22-32. PubMed PMID: 22954496.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-diabetic effects of Centratherum anthelminticum seeds methanolic fraction on pancreatic cells, β-TC6 and its alleviating role in type 2 diabetic rats. AU - Arya,Aditya, AU - Looi,Chung Yeng, AU - Cheah,Shiau Chuen, AU - Mustafa,Mohd Rais, AU - Mohd,Mustafa Ali, Y1 - 2012/08/31/ PY - 2012/06/03/received PY - 2012/07/28/revised PY - 2012/08/13/accepted PY - 2012/9/8/entrez PY - 2012/9/8/pubmed PY - 2013/3/15/medline SP - 22 EP - 32 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 144 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Seeds of Centratherum anthelminticum (Asteraceae) have been popularly used in Ayurvedic medicine to treat diabetes and skin disorders. Folk medicine from Rayalaseema (Andhra Pradesh, India) reported wide spread usage in diabetes. AIM OF THE STUDY: To investigate the hypoglycemic properties and mechanism of the methanolic fraction of C. anthelminticum seeds (CAMFs) on mouse β-TC6 pancreatic cell line and streptozotocin (STZ)-induced diabetic rat models. MATERIALS AND METHODS: We investigated the crude methanolic fraction of C. anthelminticum seeds (CAMFs) on β-TC6 cell line and confirmed its effects on type 1 and type 2 diabetic rats to understand its mechanism in managing diabetes mellitus. CAMFs were initially tested on β-TC6 cells for cytotoxicity, 2-NBDG glucose uptake, insulin secretion and glucose transporter (GLUT-1, 2 and 4) protein expression. Furthermore, streptozotocin (STZ)-induced type 1 diabetic and STZ-nicotinamide-induced type 2 diabetic rats were intraperitoneally (i.p) injected or administered orally with CAMFs daily for 28 days. The effect of CAMFs on blood glucose and insulin levels was subsequently evaluated. RESULTS: In cell line studies, CAMFs showed non-cytotoxic effect on β-TC6 cell proliferation compared to untreated control cells at 50 μg/ml. CAMFs increased glucose uptake and insulin secretion dose-dependently by up-regulating GLUT-2 and GLUT-4 expression in these cells. Further in vivo studies on streptozotocin induced diabetic rat models revealed that CAMFs significantly reduced hyperglycemia by augmenting insulin secretion in type 2 diabetic rats. However, CAMFs displayed less significant effects on type 1 diabetic rats. CONCLUSIONS: CAMFs demonstrated anti-diabetic potential on β-TC6 cells and type 2 diabetic rat model, plausibly through enhancing glucose uptake and insulin secretion. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22954496/Anti_diabetic_effects_of_Centratherum_anthelminticum_seeds_methanolic_fraction_on_pancreatic_cells_β_TC6_and_its_alleviating_role_in_type_2_diabetic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00539-9 DB - PRIME DP - Unbound Medicine ER -