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Determinants of the essential one-carbon metabolism metabolites, homocysteine, S-adenosylmethionine, S-adenosylhomocysteine and folate, in cerebrospinal fluid.
Clin Chem Lab Med. 2012 Mar 24; 50(9):1641-7.CC

Abstract

BACKGROUND

Disturbances in the levels of one-carbon (1C) metabolism metabolites have been associated with a wide variety of neuropsychiatric diseases. Cerebrospinal fluid (CSF) levels of homocysteine (Hcy) and the other 1C metabolites, nor their interrelatedness and putative determinants, have been studied extensively in a healthy population.

METHODS

Plasma and CSF samples from 100 individuals free from neuropsychiatric diseases were analyzed (55 male, 45 female; age 50±17 years). In blood, we measured plasma Hcy, serum folate and serum vitamin B12. In CSF, we measured total Hcy, S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH) and 5-methyltetrahydrofolate (5-methylTHF). Highly selective analytical methods like liquid chromatography combined with either mass spectrometry or fluorescence detection were used.

RESULTS

CSF Hcy was inversely correlated with CSF 5-methylTHF and positively with plasma Hcy, independent of serum folate status. CSF SAH correlated with age, lower CSF 5-methylTHF and higher CSF Hcy. CSF 5-methylTHF showed independent negative correlations with age and positive correlations with serum folate. CSF SAM did not correlate with any of the 1C metabolites.

CONCLUSIONS

Aging is characterized by a reduction in CSF 5-methylTHF levels and increased CSF levels of the potentially neurotoxic transmethylation inhibitor SAH. CSF 5-methylTHF, which is itself determined in part by systemic folate status, is a powerful independent determinant of CSF levels of Hcy and SAH.

Authors+Show Affiliations

Department of Clinical Chemistry, Metabolic Laboratory, VU University Medical Center, Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22962226

Citation

Smith, Desirée E C., et al. "Determinants of the Essential One-carbon Metabolism Metabolites, Homocysteine, S-adenosylmethionine, S-adenosylhomocysteine and Folate, in Cerebrospinal Fluid." Clinical Chemistry and Laboratory Medicine, vol. 50, no. 9, 2012, pp. 1641-7.
Smith DE, Smulders YM, Blom HJ, et al. Determinants of the essential one-carbon metabolism metabolites, homocysteine, S-adenosylmethionine, S-adenosylhomocysteine and folate, in cerebrospinal fluid. Clin Chem Lab Med. 2012;50(9):1641-7.
Smith, D. E., Smulders, Y. M., Blom, H. J., Popp, J., Jessen, F., Semmler, A., Farkas, M., & Linnebank, M. (2012). Determinants of the essential one-carbon metabolism metabolites, homocysteine, S-adenosylmethionine, S-adenosylhomocysteine and folate, in cerebrospinal fluid. Clinical Chemistry and Laboratory Medicine, 50(9), 1641-7. https://doi.org/10.1515/cclm-2012-0056
Smith DE, et al. Determinants of the Essential One-carbon Metabolism Metabolites, Homocysteine, S-adenosylmethionine, S-adenosylhomocysteine and Folate, in Cerebrospinal Fluid. Clin Chem Lab Med. 2012 Mar 24;50(9):1641-7. PubMed PMID: 22962226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determinants of the essential one-carbon metabolism metabolites, homocysteine, S-adenosylmethionine, S-adenosylhomocysteine and folate, in cerebrospinal fluid. AU - Smith,Desirée E C, AU - Smulders,Yvo M, AU - Blom,Henk J, AU - Popp,Julius, AU - Jessen,Frank, AU - Semmler,Alexander, AU - Farkas,Melinda, AU - Linnebank,Michael, Y1 - 2012/03/24/ PY - 2011/08/18/received PY - 2012/02/27/accepted PY - 2012/9/11/entrez PY - 2012/9/11/pubmed PY - 2013/1/17/medline SP - 1641 EP - 7 JF - Clinical chemistry and laboratory medicine JO - Clin Chem Lab Med VL - 50 IS - 9 N2 - BACKGROUND: Disturbances in the levels of one-carbon (1C) metabolism metabolites have been associated with a wide variety of neuropsychiatric diseases. Cerebrospinal fluid (CSF) levels of homocysteine (Hcy) and the other 1C metabolites, nor their interrelatedness and putative determinants, have been studied extensively in a healthy population. METHODS: Plasma and CSF samples from 100 individuals free from neuropsychiatric diseases were analyzed (55 male, 45 female; age 50±17 years). In blood, we measured plasma Hcy, serum folate and serum vitamin B12. In CSF, we measured total Hcy, S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH) and 5-methyltetrahydrofolate (5-methylTHF). Highly selective analytical methods like liquid chromatography combined with either mass spectrometry or fluorescence detection were used. RESULTS: CSF Hcy was inversely correlated with CSF 5-methylTHF and positively with plasma Hcy, independent of serum folate status. CSF SAH correlated with age, lower CSF 5-methylTHF and higher CSF Hcy. CSF 5-methylTHF showed independent negative correlations with age and positive correlations with serum folate. CSF SAM did not correlate with any of the 1C metabolites. CONCLUSIONS: Aging is characterized by a reduction in CSF 5-methylTHF levels and increased CSF levels of the potentially neurotoxic transmethylation inhibitor SAH. CSF 5-methylTHF, which is itself determined in part by systemic folate status, is a powerful independent determinant of CSF levels of Hcy and SAH. SN - 1437-4331 UR - https://www.unboundmedicine.com/medline/citation/22962226/Determinants_of_the_essential_one_carbon_metabolism_metabolites_homocysteine_S_adenosylmethionine_S_adenosylhomocysteine_and_folate_in_cerebrospinal_fluid_ L2 - https://www.degruyter.com/document/doi/10.1515/cclm-2012-0056 DB - PRIME DP - Unbound Medicine ER -