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Determinants of the essential one-carbon metabolism metabolites, homocysteine, S-adenosylmethionine, S-adenosylhomocysteine and folate, in cerebrospinal fluid.
Clin Chem Lab Med. 2012 Mar 24; 50(9):1641-7.CC

Abstract

BACKGROUND

Disturbances in the levels of one-carbon (1C) metabolism metabolites have been associated with a wide variety of neuropsychiatric diseases. Cerebrospinal fluid (CSF) levels of homocysteine (Hcy) and the other 1C metabolites, nor their interrelatedness and putative determinants, have been studied extensively in a healthy population.

METHODS

Plasma and CSF samples from 100 individuals free from neuropsychiatric diseases were analyzed (55 male, 45 female; age 50±17 years). In blood, we measured plasma Hcy, serum folate and serum vitamin B12. In CSF, we measured total Hcy, S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH) and 5-methyltetrahydrofolate (5-methylTHF). Highly selective analytical methods like liquid chromatography combined with either mass spectrometry or fluorescence detection were used.

RESULTS

CSF Hcy was inversely correlated with CSF 5-methylTHF and positively with plasma Hcy, independent of serum folate status. CSF SAH correlated with age, lower CSF 5-methylTHF and higher CSF Hcy. CSF 5-methylTHF showed independent negative correlations with age and positive correlations with serum folate. CSF SAM did not correlate with any of the 1C metabolites.

CONCLUSIONS

Aging is characterized by a reduction in CSF 5-methylTHF levels and increased CSF levels of the potentially neurotoxic transmethylation inhibitor SAH. CSF 5-methylTHF, which is itself determined in part by systemic folate status, is a powerful independent determinant of CSF levels of Hcy and SAH.

Links

Publisher Full Text
degruyter.com
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Authors+Show Affiliations

Smith DE
Department of Clinical Chemistry, Metabolic Laboratory, VU University Medical Center, Amsterdam, The Netherlands.
Smulders YM
No affiliation info available
Blom HJ
No affiliation info available
Popp J
No affiliation info available
Jessen F
No affiliation info available
Semmler A
No affiliation info available
Farkas M
No affiliation info available
Linnebank M
No affiliation info available

MeSH

AdultAgedAgingChromatography, High Pressure LiquidFemaleFolic AcidHomocysteineHumansImmunoassayMaleMiddle AgedS-AdenosylhomocysteineS-AdenosylmethionineTandem Mass SpectrometryVitamin B 12

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22962226

Citation

Smith, Desirée E C., et al. "Determinants of the Essential One-carbon Metabolism Metabolites, Homocysteine, S-adenosylmethionine, S-adenosylhomocysteine and Folate, in Cerebrospinal Fluid." Clinical Chemistry and Laboratory Medicine, vol. 50, no. 9, 2012, pp. 1641-7.
Smith DE, Smulders YM, Blom HJ, et al. Determinants of the essential one-carbon metabolism metabolites, homocysteine, S-adenosylmethionine, S-adenosylhomocysteine and folate, in cerebrospinal fluid. Clin Chem Lab Med. 2012;50(9):1641-7.
Smith, D. E., Smulders, Y. M., Blom, H. J., Popp, J., Jessen, F., Semmler, A., Farkas, M., & Linnebank, M. (2012). Determinants of the essential one-carbon metabolism metabolites, homocysteine, S-adenosylmethionine, S-adenosylhomocysteine and folate, in cerebrospinal fluid. Clinical Chemistry and Laboratory Medicine, 50(9), 1641-7. https://doi.org/10.1515/cclm-2012-0056
Smith DE, et al. Determinants of the Essential One-carbon Metabolism Metabolites, Homocysteine, S-adenosylmethionine, S-adenosylhomocysteine and Folate, in Cerebrospinal Fluid. Clin Chem Lab Med. 2012 Mar 24;50(9):1641-7. PubMed PMID: 22962226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determinants of the essential one-carbon metabolism metabolites, homocysteine, S-adenosylmethionine, S-adenosylhomocysteine and folate, in cerebrospinal fluid. AU - Smith,Desirée E C, AU - Smulders,Yvo M, AU - Blom,Henk J, AU - Popp,Julius, AU - Jessen,Frank, AU - Semmler,Alexander, AU - Farkas,Melinda, AU - Linnebank,Michael, Y1 - 2012/03/24/ PY - 2011/08/18/received PY - 2012/02/27/accepted PY - 2012/9/11/entrez PY - 2012/9/11/pubmed PY - 2013/1/17/medline SP - 1641 EP - 7 JF - Clinical chemistry and laboratory medicine JO - Clin Chem Lab Med VL - 50 IS - 9 N2 - BACKGROUND: Disturbances in the levels of one-carbon (1C) metabolism metabolites have been associated with a wide variety of neuropsychiatric diseases. Cerebrospinal fluid (CSF) levels of homocysteine (Hcy) and the other 1C metabolites, nor their interrelatedness and putative determinants, have been studied extensively in a healthy population. METHODS: Plasma and CSF samples from 100 individuals free from neuropsychiatric diseases were analyzed (55 male, 45 female; age 50±17 years). In blood, we measured plasma Hcy, serum folate and serum vitamin B12. In CSF, we measured total Hcy, S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH) and 5-methyltetrahydrofolate (5-methylTHF). Highly selective analytical methods like liquid chromatography combined with either mass spectrometry or fluorescence detection were used. RESULTS: CSF Hcy was inversely correlated with CSF 5-methylTHF and positively with plasma Hcy, independent of serum folate status. CSF SAH correlated with age, lower CSF 5-methylTHF and higher CSF Hcy. CSF 5-methylTHF showed independent negative correlations with age and positive correlations with serum folate. CSF SAM did not correlate with any of the 1C metabolites. CONCLUSIONS: Aging is characterized by a reduction in CSF 5-methylTHF levels and increased CSF levels of the potentially neurotoxic transmethylation inhibitor SAH. CSF 5-methylTHF, which is itself determined in part by systemic folate status, is a powerful independent determinant of CSF levels of Hcy and SAH. SN - 1437-4331 UR - https://www.unboundmedicine.com/medline/citation/22962226/Determinants_of_the_essential_one_carbon_metabolism_metabolites_homocysteine_S_adenosylmethionine_S_adenosylhomocysteine_and_folate_in_cerebrospinal_fluid_ L2 - https://www.degruyter.com/document/doi/10.1515/cclm-2012-0056 DB - PRIME DP - Unbound Medicine ER -