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Sevoflurane exposure in 7-day-old rats affects neurogenesis, neurodegeneration and neurocognitive function.
Neurosci Bull. 2012 Oct; 28(5):499-508.NB

Abstract

OBJECTIVE

Sevoflurane is widely used in pediatric anesthesia and former studies showed that it causes neurodegeneration in the developing brain. The present study was carried out to investigate the effects of sevoflurane on neurogenesis, neurodegeneration and behavior.

METHODS

We administered 5-bromodeoxyuridine, an S-phase marker, before, during, and after 4 h of sevoflurane given to rats on postnatal day 7 to assess dentate gyrus progenitor proliferation and Fluoro-Jade staining for degeneration. Spatial reference memory was tested 2 and 6 weeks after anesthesia.

RESULTS

Sevoflurane decreased progenitor proliferation and increased cell death until at least 4 days after anesthesia. Spatial reference memory was not affected at 2 weeks but was affected at 6 weeks after sevoflurane administration.

CONCLUSION

Sevoflurane reduces neurogenesis and increases the death of progenitor cells in developing brain. This might mediate the late-onset neurocognitive outcome after sevoflurane application.

Authors+Show Affiliations

Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22965743

Citation

Fang, Fang, et al. "Sevoflurane Exposure in 7-day-old Rats Affects Neurogenesis, Neurodegeneration and Neurocognitive Function." Neuroscience Bulletin, vol. 28, no. 5, 2012, pp. 499-508.
Fang F, Xue Z, Cang J. Sevoflurane exposure in 7-day-old rats affects neurogenesis, neurodegeneration and neurocognitive function. Neurosci Bull. 2012;28(5):499-508.
Fang, F., Xue, Z., & Cang, J. (2012). Sevoflurane exposure in 7-day-old rats affects neurogenesis, neurodegeneration and neurocognitive function. Neuroscience Bulletin, 28(5), 499-508. https://doi.org/10.1007/s12264-012-1260-4
Fang F, Xue Z, Cang J. Sevoflurane Exposure in 7-day-old Rats Affects Neurogenesis, Neurodegeneration and Neurocognitive Function. Neurosci Bull. 2012;28(5):499-508. PubMed PMID: 22965743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sevoflurane exposure in 7-day-old rats affects neurogenesis, neurodegeneration and neurocognitive function. AU - Fang,Fang, AU - Xue,Zhanggang, AU - Cang,Jing, Y1 - 2012/09/11/ PY - 2012/01/13/received PY - 2012/02/15/accepted PY - 2012/9/12/entrez PY - 2012/9/12/pubmed PY - 2013/3/27/medline SP - 499 EP - 508 JF - Neuroscience bulletin JO - Neurosci Bull VL - 28 IS - 5 N2 - OBJECTIVE: Sevoflurane is widely used in pediatric anesthesia and former studies showed that it causes neurodegeneration in the developing brain. The present study was carried out to investigate the effects of sevoflurane on neurogenesis, neurodegeneration and behavior. METHODS: We administered 5-bromodeoxyuridine, an S-phase marker, before, during, and after 4 h of sevoflurane given to rats on postnatal day 7 to assess dentate gyrus progenitor proliferation and Fluoro-Jade staining for degeneration. Spatial reference memory was tested 2 and 6 weeks after anesthesia. RESULTS: Sevoflurane decreased progenitor proliferation and increased cell death until at least 4 days after anesthesia. Spatial reference memory was not affected at 2 weeks but was affected at 6 weeks after sevoflurane administration. CONCLUSION: Sevoflurane reduces neurogenesis and increases the death of progenitor cells in developing brain. This might mediate the late-onset neurocognitive outcome after sevoflurane application. SN - 1995-8218 UR - https://www.unboundmedicine.com/medline/citation/22965743/Sevoflurane_exposure_in_7_day_old_rats_affects_neurogenesis_neurodegeneration_and_neurocognitive_function_ L2 - https://dx.doi.org/10.1007/s12264-012-1260-4 DB - PRIME DP - Unbound Medicine ER -