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Reduction of gastrointestinal motility by unilateral thyroparathyroidectomy plus subdiaphragmatic vagotomy in rats.

Abstract

AIM

To investigate whether the combined methods of unilateral thyroparathyroidectomy (TPX) and subdiaphragmatic vagotomy (VAX) can be adapted for rats and used as a reliable method to produce a rat model of long-term reduction of gastrointestinal (GI) motor function.

METHODS

Male Sprague-Dawley rats were randomly divided into 3 groups, normal, sham-operated and unilateral TPX plus VAX. The TPX plus VAX rats received VAX 7 d after application of TPX, and dietary intake and fecal output were then measured daily for 1 wk. After completion of the experiments, gastric emptying and small bowel transit were measured in vivo, and the contractile responses of colonic strips to excitatory and inhibitory neurotransmitters were estimated using isometric force transducers in vitro.

RESULTS

In comparison with normal and sham-operated rats, rats which received unilateral TPX plus VAX showed a significant decrease in body weight and in fecal pellet number and weight throughout the entire week. Application of TPX plus VAX to rats markedly delayed gastric emptying and small bowel transit. In TPX plus VAX rats, the longitudinal muscles of the proximal colon showed a significant reduction in contractile responses to acetylcholine (5 × 10(-6) mol/L), and a dramatic attenuation of contractile responses was also observed in both the longitudinal and circular muscles of the distal colon. However, the spontaneous contractility of the colonic strips from TPX plus VAX rats was not significantly affected by treatment with N-nitro-L-arginine-methyl ester (0.1 mol/L).

CONCLUSION

The results indicate that unilateral TPX plus VAX reduced the motor function of the GI tract in rats, and the reduced gut motility is likely mediated, at least in part, by inhibition of the excitatory neurotransmitter system.

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  • Authors+Show Affiliations

    ,

    Department of Pharmacology, Wonkwang University School of Medicine, Jeonbuk 570-749, South Korea.

    , , , , , ,

    Source

    World journal of gastroenterology 18:33 2012 Sep 07 pg 4570-7

    MeSH

    Acetylcholine
    Animals
    Colon
    Defecation
    Diaphragm
    Gastric Emptying
    Gastrointestinal Motility
    Gastrointestinal Transit
    Male
    Models, Animal
    Muscle Contraction
    Muscle, Smooth
    Parathyroid Glands
    Parathyroidectomy
    Rats
    Rats, Sprague-Dawley
    Thyroxine
    Vagotomy
    Vagus Nerve
    Vasodilator Agents

    Pub Type(s)

    Comparative Study
    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22969231

    Citation

    Lee, Jun Ho, et al. "Reduction of Gastrointestinal Motility By Unilateral Thyroparathyroidectomy Plus Subdiaphragmatic Vagotomy in Rats." World Journal of Gastroenterology, vol. 18, no. 33, 2012, pp. 4570-7.
    Lee JH, Kwon OD, Ahn SH, et al. Reduction of gastrointestinal motility by unilateral thyroparathyroidectomy plus subdiaphragmatic vagotomy in rats. World J Gastroenterol. 2012;18(33):4570-7.
    Lee, J. H., Kwon, O. D., Ahn, S. H., Choi, K. H., Park, J. H., Lee, S., ... Jung, K. Y. (2012). Reduction of gastrointestinal motility by unilateral thyroparathyroidectomy plus subdiaphragmatic vagotomy in rats. World Journal of Gastroenterology, 18(33), pp. 4570-7. doi:10.3748/wjg.v18.i33.4570.
    Lee JH, et al. Reduction of Gastrointestinal Motility By Unilateral Thyroparathyroidectomy Plus Subdiaphragmatic Vagotomy in Rats. World J Gastroenterol. 2012 Sep 7;18(33):4570-7. PubMed PMID: 22969231.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Reduction of gastrointestinal motility by unilateral thyroparathyroidectomy plus subdiaphragmatic vagotomy in rats. AU - Lee,Jun Ho, AU - Kwon,Oh Deog, AU - Ahn,Seon Ho, AU - Choi,Keun Han, AU - Park,Ji Hye, AU - Lee,Seoul, AU - Choi,Bong Kyu, AU - Jung,Kyu Yong, PY - 2012/01/13/received PY - 2012/03/02/revised PY - 2012/03/20/accepted PY - 2012/9/13/entrez PY - 2012/9/13/pubmed PY - 2013/4/17/medline KW - Gastric emptying KW - Rat KW - Small bowel transit KW - Subdiaphragmatic vagotomy KW - Unilateral thyroparathyroidectomy SP - 4570 EP - 7 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 18 IS - 33 N2 - AIM: To investigate whether the combined methods of unilateral thyroparathyroidectomy (TPX) and subdiaphragmatic vagotomy (VAX) can be adapted for rats and used as a reliable method to produce a rat model of long-term reduction of gastrointestinal (GI) motor function. METHODS: Male Sprague-Dawley rats were randomly divided into 3 groups, normal, sham-operated and unilateral TPX plus VAX. The TPX plus VAX rats received VAX 7 d after application of TPX, and dietary intake and fecal output were then measured daily for 1 wk. After completion of the experiments, gastric emptying and small bowel transit were measured in vivo, and the contractile responses of colonic strips to excitatory and inhibitory neurotransmitters were estimated using isometric force transducers in vitro. RESULTS: In comparison with normal and sham-operated rats, rats which received unilateral TPX plus VAX showed a significant decrease in body weight and in fecal pellet number and weight throughout the entire week. Application of TPX plus VAX to rats markedly delayed gastric emptying and small bowel transit. In TPX plus VAX rats, the longitudinal muscles of the proximal colon showed a significant reduction in contractile responses to acetylcholine (5 × 10(-6) mol/L), and a dramatic attenuation of contractile responses was also observed in both the longitudinal and circular muscles of the distal colon. However, the spontaneous contractility of the colonic strips from TPX plus VAX rats was not significantly affected by treatment with N-nitro-L-arginine-methyl ester (0.1 mol/L). CONCLUSION: The results indicate that unilateral TPX plus VAX reduced the motor function of the GI tract in rats, and the reduced gut motility is likely mediated, at least in part, by inhibition of the excitatory neurotransmitter system. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/22969231/Reduction_of_gastrointestinal_motility_by_unilateral_thyroparathyroidectomy_plus_subdiaphragmatic_vagotomy_in_rats L2 - http://www.wjgnet.com/1007-9327/full/v18/i33/4570.htm DB - PRIME DP - Unbound Medicine ER -