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Topical application of an ethanol extract prepared from Illicium verum suppresses atopic dermatitis in NC/Nga mice.
J Ethnopharmacol. 2012 Oct 31; 144(1):151-9.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Illicium verum is a traditional herbal medicine with anti-inflammatory properties used in Asia. However, its usefulness in the treatment of allergic diseases remains unclear. This study evaluated the anti-inflammatory and antiallergic effects of I. verum extract (IVE) in a mouse model of atopic dermatitis.

MATERIALS AND METHODS

We investigated the effects of IVE on compound 48/80-induced histamine release, and phorbol 12-myristate13-acetate and calcium ionophore A23187-stimulated cytokines secretion in MC/9 mast cells. Atopic dermatitis was induced in NC/Nga mice by exposure to extract of house dust mite (Dermatophagoides farinae). After a topical application of IVE on ear and skin lesions, we evaluated the severity of skin symptoms, ear thickness, inflammatory cell infiltration, and serum levels of immunoglobulin E (IgE), histamine, interleukin (IL)-6, and intercellular adhesion molecule (ICAM)-1. In addition, we determined the expression of IL-4, IL-6, tumor necrosis factor (TNF)-α, interferon (IFN)-γ thymus- and activation-regulated chemokine (TARC), regulated on activation, normal T cell expressed and secreted (RANTES), ICAM-1, and vascular cell adhesion molecule (VCAM)-1 in ear tissues.

RESULTS

IVE inhibited secretion of histamine, IL-4, IL-6, and TNF-α from mast cells in a dose-dependent manner. Topical application of IVE significantly reduced dermatitis scores, ear thickness, and serum levels of IgE, histamine, IL-6, and ICAM-1. Histopathological analysis demonstrated decreased epidermal thickening and dermal infiltration by inflammatory cells. In the ear lesions, IVE treatment reduced expression of IL-4, IL-6, TNF-α, TARC, RANTES, ICAM-1, and VCAM-1, but not IFN-γ.

CONCLUSIONS

These results indicate that IVE inhibits atopic dermatitis-like skin lesions by suppressing the expression of cytokines, chemokines, and adhesion molecules. These results suggest that IVE may be a potential therapeutic candidate for atopic dermatitis.

Authors+Show Affiliations

Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine, 483 Expo-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22971899

Citation

Sung, Yoon-Young, et al. "Topical Application of an Ethanol Extract Prepared From Illicium Verum Suppresses Atopic Dermatitis in NC/Nga Mice." Journal of Ethnopharmacology, vol. 144, no. 1, 2012, pp. 151-9.
Sung YY, Yang WK, Lee AY, et al. Topical application of an ethanol extract prepared from Illicium verum suppresses atopic dermatitis in NC/Nga mice. J Ethnopharmacol. 2012;144(1):151-9.
Sung, Y. Y., Yang, W. K., Lee, A. Y., Kim, D. S., Nho, K. J., Kim, Y. S., & Kim, H. K. (2012). Topical application of an ethanol extract prepared from Illicium verum suppresses atopic dermatitis in NC/Nga mice. Journal of Ethnopharmacology, 144(1), 151-9. https://doi.org/10.1016/j.jep.2012.08.042
Sung YY, et al. Topical Application of an Ethanol Extract Prepared From Illicium Verum Suppresses Atopic Dermatitis in NC/Nga Mice. J Ethnopharmacol. 2012 Oct 31;144(1):151-9. PubMed PMID: 22971899.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Topical application of an ethanol extract prepared from Illicium verum suppresses atopic dermatitis in NC/Nga mice. AU - Sung,Yoon-Young, AU - Yang,Won-Kyung, AU - Lee,A Yeong, AU - Kim,Dong-Seon, AU - Nho,Kyoung Jin, AU - Kim,Young Sang, AU - Kim,Ho Kyoung, Y1 - 2012/09/03/ PY - 2012/04/24/received PY - 2012/08/24/revised PY - 2012/08/27/accepted PY - 2012/9/14/entrez PY - 2012/9/14/pubmed PY - 2013/3/15/medline SP - 151 EP - 9 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 144 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Illicium verum is a traditional herbal medicine with anti-inflammatory properties used in Asia. However, its usefulness in the treatment of allergic diseases remains unclear. This study evaluated the anti-inflammatory and antiallergic effects of I. verum extract (IVE) in a mouse model of atopic dermatitis. MATERIALS AND METHODS: We investigated the effects of IVE on compound 48/80-induced histamine release, and phorbol 12-myristate13-acetate and calcium ionophore A23187-stimulated cytokines secretion in MC/9 mast cells. Atopic dermatitis was induced in NC/Nga mice by exposure to extract of house dust mite (Dermatophagoides farinae). After a topical application of IVE on ear and skin lesions, we evaluated the severity of skin symptoms, ear thickness, inflammatory cell infiltration, and serum levels of immunoglobulin E (IgE), histamine, interleukin (IL)-6, and intercellular adhesion molecule (ICAM)-1. In addition, we determined the expression of IL-4, IL-6, tumor necrosis factor (TNF)-α, interferon (IFN)-γ thymus- and activation-regulated chemokine (TARC), regulated on activation, normal T cell expressed and secreted (RANTES), ICAM-1, and vascular cell adhesion molecule (VCAM)-1 in ear tissues. RESULTS: IVE inhibited secretion of histamine, IL-4, IL-6, and TNF-α from mast cells in a dose-dependent manner. Topical application of IVE significantly reduced dermatitis scores, ear thickness, and serum levels of IgE, histamine, IL-6, and ICAM-1. Histopathological analysis demonstrated decreased epidermal thickening and dermal infiltration by inflammatory cells. In the ear lesions, IVE treatment reduced expression of IL-4, IL-6, TNF-α, TARC, RANTES, ICAM-1, and VCAM-1, but not IFN-γ. CONCLUSIONS: These results indicate that IVE inhibits atopic dermatitis-like skin lesions by suppressing the expression of cytokines, chemokines, and adhesion molecules. These results suggest that IVE may be a potential therapeutic candidate for atopic dermatitis. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22971899/Topical_application_of_an_ethanol_extract_prepared_from_Illicium_verum_suppresses_atopic_dermatitis_in_NC/Nga_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00567-3 DB - PRIME DP - Unbound Medicine ER -