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Phenotypic analysis of Salmonella enterica serovar Typhimurium rpoE mutants encoding RNA polymerase extracytoplasmic stress response sigma factors σ(E) with altered promoter specificity.
Arch Microbiol. 2013 Jan; 195(1):27-36.AM

Abstract

We previously identified mutants in the rpoE gene of Salmonella enterica serovar Typhimurium (S. Typhimurium) encoding RNA polymerase extracytoplasmic stress response sigma factors σ(E) with altered promoter specificity. The replacement of the conserved R171 residue in the conserved region 4.2 of σ(E) by different amino acid residues exhibited different phenotypes. While R171A almost completely abolished sigma factor activity, R171G and R171C mutant changes imparted a relaxed recognition phenotype to the sigma factor. In the present study, we introduced these mutations into the S. Typhimurium chromosome to investigate their phenotype during ethanol stress and in promoter recognition. Both relaxed sigma factors were found to initiate transcription from a high number of artificial promoters in the S. Typhimurium genome. Both mutants had substantially decreased activity under stress conditions. However, this decreased activity and also the recognition of atypical promoters had no significant effect upon growth, even in stressful conditions.

Authors+Show Affiliations

Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22972381

Citation

Rezuchova, Bronislava, et al. "Phenotypic Analysis of Salmonella Enterica Serovar Typhimurium rpoE Mutants Encoding RNA Polymerase Extracytoplasmic Stress Response Sigma Factors σ(E) With Altered Promoter Specificity." Archives of Microbiology, vol. 195, no. 1, 2013, pp. 27-36.
Rezuchova B, Homerova D, Sevcikova B, et al. Phenotypic analysis of Salmonella enterica serovar Typhimurium rpoE mutants encoding RNA polymerase extracytoplasmic stress response sigma factors σ(E) with altered promoter specificity. Arch Microbiol. 2013;195(1):27-36.
Rezuchova, B., Homerova, D., Sevcikova, B., Novakova, R., Feckova, L., Roberts, M., & Kormanec, J. (2013). Phenotypic analysis of Salmonella enterica serovar Typhimurium rpoE mutants encoding RNA polymerase extracytoplasmic stress response sigma factors σ(E) with altered promoter specificity. Archives of Microbiology, 195(1), 27-36. https://doi.org/10.1007/s00203-012-0843-9
Rezuchova B, et al. Phenotypic Analysis of Salmonella Enterica Serovar Typhimurium rpoE Mutants Encoding RNA Polymerase Extracytoplasmic Stress Response Sigma Factors σ(E) With Altered Promoter Specificity. Arch Microbiol. 2013;195(1):27-36. PubMed PMID: 22972381.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic analysis of Salmonella enterica serovar Typhimurium rpoE mutants encoding RNA polymerase extracytoplasmic stress response sigma factors σ(E) with altered promoter specificity. AU - Rezuchova,Bronislava, AU - Homerova,Dagmar, AU - Sevcikova,Beatrica, AU - Novakova,Renata, AU - Feckova,Lubomira, AU - Roberts,Mark, AU - Kormanec,Jan, Y1 - 2012/09/13/ PY - 2012/06/13/received PY - 2012/08/31/accepted PY - 2012/08/29/revised PY - 2012/9/14/entrez PY - 2012/9/14/pubmed PY - 2013/4/9/medline SP - 27 EP - 36 JF - Archives of microbiology JO - Arch Microbiol VL - 195 IS - 1 N2 - We previously identified mutants in the rpoE gene of Salmonella enterica serovar Typhimurium (S. Typhimurium) encoding RNA polymerase extracytoplasmic stress response sigma factors σ(E) with altered promoter specificity. The replacement of the conserved R171 residue in the conserved region 4.2 of σ(E) by different amino acid residues exhibited different phenotypes. While R171A almost completely abolished sigma factor activity, R171G and R171C mutant changes imparted a relaxed recognition phenotype to the sigma factor. In the present study, we introduced these mutations into the S. Typhimurium chromosome to investigate their phenotype during ethanol stress and in promoter recognition. Both relaxed sigma factors were found to initiate transcription from a high number of artificial promoters in the S. Typhimurium genome. Both mutants had substantially decreased activity under stress conditions. However, this decreased activity and also the recognition of atypical promoters had no significant effect upon growth, even in stressful conditions. SN - 1432-072X UR - https://www.unboundmedicine.com/medline/citation/22972381/Phenotypic_analysis_of_Salmonella_enterica_serovar_Typhimurium_rpoE_mutants_encoding_RNA_polymerase_extracytoplasmic_stress_response_sigma_factors_σ_E__with_altered_promoter_specificity_ L2 - https://dx.doi.org/10.1007/s00203-012-0843-9 DB - PRIME DP - Unbound Medicine ER -