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Molecular control of TiO₂-NPs toxicity formation at predicted environmental relevant concentrations by Mn-SODs proteins.
PLoS One. 2012; 7(9):e44688.Plos

Abstract

With growing concerns of the safety of nanotechnology, the in vivo toxicity of nanoparticles (NPs) at environmental relevant concentrations has drawn increasing attentions. We investigated the possible molecular mechanisms of titanium nanoparticles (Ti-NPs) in the induction of toxicity at predicted environmental relevant concentrations. In nematodes, small sizes (4 nm and 10 nm) of TiO₂-NPs induced more severe toxicities than large sizes (60 nm and 90 nm) of TiO₂-NPs on animals using lethality, growth, reproduction, locomotion behavior, intestinal autofluorescence, and reactive oxygen species (ROS) production as endpoints. Locomotion behaviors could be significantly decreased by exposure to 4-nm and 10-nm TiO₂-NPs at concentration of 1 ng/L in nematodes. Among genes required for the control of oxidative stress, only the expression patterns of sod-2 and sod-3 genes encoding Mn-SODs in animals exposed to small sizes TiO₂-NPs were significantly different from those in animals exposed to large sizes of TiO₂-NPs. sod-2 and sod-3 gene expressions were closely correlated with lethality, growth, reproduction, locomotion behavior, intestinal autofluorescence, and ROS production in TiO₂-NPs-exposed animals. Ectopically expression of human and nematode Mn-SODs genes effectively prevented the induction of ROS production and the development of toxicity of TiO₂-NPs. Therefore, the altered expression patterns of Mn-SODs may explain the toxicity formation for different sizes of TiO₂-NPs at predicted environmental relevant concentrations. In addition, we demonstrated here a strategy to investigate the toxicological effects of exposure to NPs upon humans by generating transgenic strains in nematodes for specific human genes.

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Authors+Show Affiliations

Li Y
Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Department of Biochemistry and Molecular Biology, Medical School of Southeast University, Nanjing, China.
Wang W
No affiliation info available
Wu Q
No affiliation info available
Li Y
No affiliation info available
Tang M
No affiliation info available
Ye B
No affiliation info available
Wang D
No affiliation info available

MeSH

AnimalsAnimals, Genetically ModifiedBody SizeCaenorhabditis elegansCaenorhabditis elegans ProteinsGene Expression RegulationLocomotionMetal NanoparticlesOxidative StressParticle SizeReproductionSuperoxide DismutaseSurvival AnalysisTitanium

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22973466

Citation

Li, Yinxia, et al. "Molecular Control of TiO₂-NPs Toxicity Formation at Predicted Environmental Relevant Concentrations By Mn-SODs Proteins." PloS One, vol. 7, no. 9, 2012, pp. e44688.
Li Y, Wang W, Wu Q, et al. Molecular control of TiO₂-NPs toxicity formation at predicted environmental relevant concentrations by Mn-SODs proteins. PLoS One. 2012;7(9):e44688.
Li, Y., Wang, W., Wu, Q., Li, Y., Tang, M., Ye, B., & Wang, D. (2012). Molecular control of TiO₂-NPs toxicity formation at predicted environmental relevant concentrations by Mn-SODs proteins. PloS One, 7(9), e44688. https://doi.org/10.1371/journal.pone.0044688
Li Y, et al. Molecular Control of TiO₂-NPs Toxicity Formation at Predicted Environmental Relevant Concentrations By Mn-SODs Proteins. PLoS One. 2012;7(9):e44688. PubMed PMID: 22973466.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular control of TiO₂-NPs toxicity formation at predicted environmental relevant concentrations by Mn-SODs proteins. AU - Li,Yinxia, AU - Wang,Wei, AU - Wu,Qiuli, AU - Li,Yiping, AU - Tang,Meng, AU - Ye,Boping, AU - Wang,Dayong, Y1 - 2012/09/04/ PY - 2012/05/29/received PY - 2012/08/07/accepted PY - 2012/9/14/entrez PY - 2012/9/14/pubmed PY - 2013/1/29/medline SP - e44688 EP - e44688 JF - PloS one JO - PLoS One VL - 7 IS - 9 N2 - With growing concerns of the safety of nanotechnology, the in vivo toxicity of nanoparticles (NPs) at environmental relevant concentrations has drawn increasing attentions. We investigated the possible molecular mechanisms of titanium nanoparticles (Ti-NPs) in the induction of toxicity at predicted environmental relevant concentrations. In nematodes, small sizes (4 nm and 10 nm) of TiO₂-NPs induced more severe toxicities than large sizes (60 nm and 90 nm) of TiO₂-NPs on animals using lethality, growth, reproduction, locomotion behavior, intestinal autofluorescence, and reactive oxygen species (ROS) production as endpoints. Locomotion behaviors could be significantly decreased by exposure to 4-nm and 10-nm TiO₂-NPs at concentration of 1 ng/L in nematodes. Among genes required for the control of oxidative stress, only the expression patterns of sod-2 and sod-3 genes encoding Mn-SODs in animals exposed to small sizes TiO₂-NPs were significantly different from those in animals exposed to large sizes of TiO₂-NPs. sod-2 and sod-3 gene expressions were closely correlated with lethality, growth, reproduction, locomotion behavior, intestinal autofluorescence, and ROS production in TiO₂-NPs-exposed animals. Ectopically expression of human and nematode Mn-SODs genes effectively prevented the induction of ROS production and the development of toxicity of TiO₂-NPs. Therefore, the altered expression patterns of Mn-SODs may explain the toxicity formation for different sizes of TiO₂-NPs at predicted environmental relevant concentrations. In addition, we demonstrated here a strategy to investigate the toxicological effects of exposure to NPs upon humans by generating transgenic strains in nematodes for specific human genes. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/22973466/Molecular_control_of_TiO₂_NPs_toxicity_formation_at_predicted_environmental_relevant_concentrations_by_Mn_SODs_proteins_ L2 - https://dx.plos.org/10.1371/journal.pone.0044688 DB - PRIME DP - Unbound Medicine ER -