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Role of catechins in the antioxidant capacity of an active film containing green tea, green coffee, and grapefruit extracts.
J Agric Food Chem. 2012 Oct 03; 60(39):9842-9.JA

Abstract

The oxygen radical absorbance capacity (ORAC) method was used to characterize the antioxidant capacity of natural extracts of green tea, green coffee, and grapefruit. These natural extracts were incorporated into a plastic film layer, which was subsequently subjected to a free radical gas stream in order to determine the antioxidant capacity directly in the active film. The green tea extract (GTE) afforded the strongest antioxidant activity. To identify the active compounds in the extract, concentration of the diverse catechins in samples were determined by HPLC-UV analysis. The results showed that the content of catechins in the GTE is around 77% (w/w), the major components being (-)-epigallocatechin gallate, (-)-epicatechin gallate, and (-)-epicatechin. A variation in the concentration profile of catechins was detected during the oxidation process. The chromatographic study demonstrated that (-)-gallocatechin, (-)- epigallocatechin, (+)-catechin, and (-)-catechin gallate exhibited the most radical scavenging.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Colon M
Department of Analytical Chemistry, Aragon Institute of Engineering Research I3A, CPS-University of Zaragoza , Torres Quevedo Building, María de Luna Street 3, E-50018 Zaragoza, Spain.
Nerin C
No affiliation info available

MeSH

AntioxidantsCamellia sinensisCatechinCitrus paradisiCoffeaOxidation-ReductionPlant ExtractsReactive Oxygen Species

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22973940

Citation

Colon, M, and C Nerin. "Role of Catechins in the Antioxidant Capacity of an Active Film Containing Green Tea, Green Coffee, and Grapefruit Extracts." Journal of Agricultural and Food Chemistry, vol. 60, no. 39, 2012, pp. 9842-9.
Colon M, Nerin C. Role of catechins in the antioxidant capacity of an active film containing green tea, green coffee, and grapefruit extracts. J Agric Food Chem. 2012;60(39):9842-9.
Colon, M., & Nerin, C. (2012). Role of catechins in the antioxidant capacity of an active film containing green tea, green coffee, and grapefruit extracts. Journal of Agricultural and Food Chemistry, 60(39), 9842-9. https://doi.org/10.1021/jf302477y
Colon M, Nerin C. Role of Catechins in the Antioxidant Capacity of an Active Film Containing Green Tea, Green Coffee, and Grapefruit Extracts. J Agric Food Chem. 2012 Oct 3;60(39):9842-9. PubMed PMID: 22973940.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of catechins in the antioxidant capacity of an active film containing green tea, green coffee, and grapefruit extracts. AU - Colon,M, AU - Nerin,C, Y1 - 2012/09/25/ PY - 2012/9/15/entrez PY - 2012/9/15/pubmed PY - 2013/2/23/medline SP - 9842 EP - 9 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 60 IS - 39 N2 - The oxygen radical absorbance capacity (ORAC) method was used to characterize the antioxidant capacity of natural extracts of green tea, green coffee, and grapefruit. These natural extracts were incorporated into a plastic film layer, which was subsequently subjected to a free radical gas stream in order to determine the antioxidant capacity directly in the active film. The green tea extract (GTE) afforded the strongest antioxidant activity. To identify the active compounds in the extract, concentration of the diverse catechins in samples were determined by HPLC-UV analysis. The results showed that the content of catechins in the GTE is around 77% (w/w), the major components being (-)-epigallocatechin gallate, (-)-epicatechin gallate, and (-)-epicatechin. A variation in the concentration profile of catechins was detected during the oxidation process. The chromatographic study demonstrated that (-)-gallocatechin, (-)- epigallocatechin, (+)-catechin, and (-)-catechin gallate exhibited the most radical scavenging. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/22973940/Role_of_catechins_in_the_antioxidant_capacity_of_an_active_film_containing_green_tea_green_coffee_and_grapefruit_extracts_ L2 - https://doi.org/10.1021/jf302477y DB - PRIME DP - Unbound Medicine ER -