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Idebenone treatment mediates the effect of menadione oxidative stress damage in Saccharomyces cerevisiae.
Drug Metab Lett. 2012 Jun 01; 6(2):120-3.DM

Abstract

We investigated the damage caused by oxidative stress using the yeast Saccharomyces cerevisiae as a model biological system. After inducing oxidative stress with menadione, we were able to evaluate the extent of cellular oxidative stress by utilizing 2',7'-dichlorofluorescein diacetate (DCFH-DA) as a marker of the presence of reactive oxygen species. Cells were grown on different carbon sources in order to compare fermentative and oxidative metabolism. Under these conditions we evaluated the effectiveness of idebenone (2,3-dimethoxy-5-methyl-6-(10- hydroxydecyl)-1,4-benzoquinone) as a molecule that could relieve menadione-induced growth inhibition in Saccharomyces cerevisiae.

Authors+Show Affiliations

Departamento de Bioquimica Humana, Instituto de Investigaciones Biomedicas (INBIOMED) Facultad de Medicina, Universidad de Buenos Aires, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

22974201

Citation

Gamondi, Oliver, et al. "Idebenone Treatment Mediates the Effect of Menadione Oxidative Stress Damage in Saccharomyces Cerevisiae." Drug Metabolism Letters, vol. 6, no. 2, 2012, pp. 120-3.
Gamondi O, Chapela S, Nievas I, et al. Idebenone treatment mediates the effect of menadione oxidative stress damage in Saccharomyces cerevisiae. Drug Metab Lett. 2012;6(2):120-3.
Gamondi, O., Chapela, S., Nievas, I., Burgos, I., Alonso, M., & Stella, C. (2012). Idebenone treatment mediates the effect of menadione oxidative stress damage in Saccharomyces cerevisiae. Drug Metabolism Letters, 6(2), 120-3.
Gamondi O, et al. Idebenone Treatment Mediates the Effect of Menadione Oxidative Stress Damage in Saccharomyces Cerevisiae. Drug Metab Lett. 2012 Jun 1;6(2):120-3. PubMed PMID: 22974201.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Idebenone treatment mediates the effect of menadione oxidative stress damage in Saccharomyces cerevisiae. AU - Gamondi,Oliver, AU - Chapela,Sebastián, AU - Nievas,Inés, AU - Burgos,Isabel, AU - Alonso,Manuel, AU - Stella,Carlos, PY - 2012/07/07/received PY - 2012/08/10/revised PY - 2012/09/06/accepted PY - 2012/9/15/entrez PY - 2012/9/15/pubmed PY - 2013/6/12/medline SP - 120 EP - 3 JF - Drug metabolism letters JO - Drug Metab Lett VL - 6 IS - 2 N2 - We investigated the damage caused by oxidative stress using the yeast Saccharomyces cerevisiae as a model biological system. After inducing oxidative stress with menadione, we were able to evaluate the extent of cellular oxidative stress by utilizing 2',7'-dichlorofluorescein diacetate (DCFH-DA) as a marker of the presence of reactive oxygen species. Cells were grown on different carbon sources in order to compare fermentative and oxidative metabolism. Under these conditions we evaluated the effectiveness of idebenone (2,3-dimethoxy-5-methyl-6-(10- hydroxydecyl)-1,4-benzoquinone) as a molecule that could relieve menadione-induced growth inhibition in Saccharomyces cerevisiae. SN - 1874-0758 UR - https://www.unboundmedicine.com/medline/citation/22974201/Idebenone_treatment_mediates_the_effect_of_menadione_oxidative_stress_damage_in_Saccharomyces_cerevisiae_ L2 - http://www.eurekaselect.com/104976/article DB - PRIME DP - Unbound Medicine ER -