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Neuroprotective effect of epigallocatechin-3-gallate against N-methyl-D-aspartate-induced excitotoxicity in the adult rat retina.
Acta Ophthalmol. 2012 Dec; 90(8):e609-15.AO

Abstract

PURPOSE

Epigallocatechin-3-gallate (EGCG), the major polyphenol of green tea, has been suggested to reduce glutamate excitotoxicity. We therefore investigated the potentially protective effects of EGCG against N-methyl-d-aspartate (NMDA)-induced excitotoxicity in the retina.

METHODS

Female Wistar rats (n = 171) were divided into a normal control group (n = 9); saline control group with intravitreal saline injections (n = 54); NMDA control group with an intravitreal NMDA injection and intraperitoneal saline injections (n = 54); and NMDA study group (n = 54) receiving an intravitreal NMDA injection plus intraperitoneal EGCG (25 mg/kg) injections. Starting at 2 days prior to the intravitreal NMDA injection, the intraperitoneal injections were performed daily for the whole study period. At 12 hr, 1, 2, 3 days, 1 and 2 weeks after the intravitreal NMDA injection, the animals were killed. We counted the neurons in the retinal ganglion cell layer (GCL) on histological sections, measured the thickness of Thy-1 immunoreactivity and assessed the expression of Thy-1 mRNA by real-time polymerase chain reaction.

RESULTS

At all time-points, GCL cell density, thickness of Thy-1 immunoreactivity and expression of Thy-1 mRNA were significantly (all p < 0.05) lower in the NMDA control group than in the NMDA study group, in which the parameters were significantly (all p < 0.05) lower than in the saline control group and the normal control group. In both groups with an intravitreal NMDA injection, GCL cell density, thickness of Thy-1 immunoreactivity and expression of Thy-1 mRNA decreased significantly with increasing follow-up time.

CONCLUSIONS

Intraperitoneal application of EGCG resulted in a significantly less marked NMDA-associated loss of retinal ganglion cells.

Authors+Show Affiliations

Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22974415

Citation

Chen, Fei, et al. "Neuroprotective Effect of Epigallocatechin-3-gallate Against N-methyl-D-aspartate-induced Excitotoxicity in the Adult Rat Retina." Acta Ophthalmologica, vol. 90, no. 8, 2012, pp. e609-15.
Chen F, Jiang L, Shen C, et al. Neuroprotective effect of epigallocatechin-3-gallate against N-methyl-D-aspartate-induced excitotoxicity in the adult rat retina. Acta Ophthalmol. 2012;90(8):e609-15.
Chen, F., Jiang, L., Shen, C., Wan, H., Xu, L., Wang, N., & Jonas, J. B. (2012). Neuroprotective effect of epigallocatechin-3-gallate against N-methyl-D-aspartate-induced excitotoxicity in the adult rat retina. Acta Ophthalmologica, 90(8), e609-15. https://doi.org/10.1111/j.1755-3768.2012.02502.x
Chen F, et al. Neuroprotective Effect of Epigallocatechin-3-gallate Against N-methyl-D-aspartate-induced Excitotoxicity in the Adult Rat Retina. Acta Ophthalmol. 2012;90(8):e609-15. PubMed PMID: 22974415.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotective effect of epigallocatechin-3-gallate against N-methyl-D-aspartate-induced excitotoxicity in the adult rat retina. AU - Chen,Fei, AU - Jiang,Libin, AU - Shen,Ceying, AU - Wan,Hongfei, AU - Xu,Liang, AU - Wang,Ningli, AU - Jonas,Jost B, Y1 - 2012/09/13/ PY - 2012/9/15/entrez PY - 2012/9/15/pubmed PY - 2013/3/15/medline SP - e609 EP - 15 JF - Acta ophthalmologica JO - Acta Ophthalmol VL - 90 IS - 8 N2 - PURPOSE: Epigallocatechin-3-gallate (EGCG), the major polyphenol of green tea, has been suggested to reduce glutamate excitotoxicity. We therefore investigated the potentially protective effects of EGCG against N-methyl-d-aspartate (NMDA)-induced excitotoxicity in the retina. METHODS: Female Wistar rats (n = 171) were divided into a normal control group (n = 9); saline control group with intravitreal saline injections (n = 54); NMDA control group with an intravitreal NMDA injection and intraperitoneal saline injections (n = 54); and NMDA study group (n = 54) receiving an intravitreal NMDA injection plus intraperitoneal EGCG (25 mg/kg) injections. Starting at 2 days prior to the intravitreal NMDA injection, the intraperitoneal injections were performed daily for the whole study period. At 12 hr, 1, 2, 3 days, 1 and 2 weeks after the intravitreal NMDA injection, the animals were killed. We counted the neurons in the retinal ganglion cell layer (GCL) on histological sections, measured the thickness of Thy-1 immunoreactivity and assessed the expression of Thy-1 mRNA by real-time polymerase chain reaction. RESULTS: At all time-points, GCL cell density, thickness of Thy-1 immunoreactivity and expression of Thy-1 mRNA were significantly (all p < 0.05) lower in the NMDA control group than in the NMDA study group, in which the parameters were significantly (all p < 0.05) lower than in the saline control group and the normal control group. In both groups with an intravitreal NMDA injection, GCL cell density, thickness of Thy-1 immunoreactivity and expression of Thy-1 mRNA decreased significantly with increasing follow-up time. CONCLUSIONS: Intraperitoneal application of EGCG resulted in a significantly less marked NMDA-associated loss of retinal ganglion cells. SN - 1755-3768 UR - https://www.unboundmedicine.com/medline/citation/22974415/Neuroprotective_effect_of_epigallocatechin_3_gallate_against_N_methyl_D_aspartate_induced_excitotoxicity_in_the_adult_rat_retina_ L2 - https://doi.org/10.1111/j.1755-3768.2012.02502.x DB - PRIME DP - Unbound Medicine ER -