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Activation of R-mediated innate immunity and disease susceptibility is affected by mutations in a cytosolic O-acetylserine (thiol) lyase in Arabidopsis.
Plant J. 2013 Jan; 73(1):118-30.PJ

Abstract

O-acetylserine (thiol) lyases (OASTLs) are evolutionarily conserved proteins among many prokaryotes and eukaryotes that perform sulfur acquisition and synthesis of cysteine. A mutation in the cytosolic OASTL-A1 protein ONSET OF LEAF DEATH3 (OLD3) was previously shown to reduce the OASTL activity of the old3-1 protein in vitro and cause auto-necrosis in specific Arabidopsis accessions. Here we investigated why a mutation in this protein causes auto-necrosis in some but not other accessions. The auto-necrosis was found to depend on Recognition of Peronospora Parasitica 1 (RPP1)-like disease resistance R gene(s) from an evolutionarily divergent R gene cluster that is present in Ler-0 but not the reference accession Col-0. RPP1-like gene(s) show a negative epistatic interaction with the old3-1 mutation that is not linked to reduced cysteine biosynthesis. Metabolic profiling and transcriptional analysis further indicate that an effector triggered-like immune response and metabolic disorder are associated with auto-necrosis in old3-1 mutants, probably activated by an RPP1-like gene. However, the old3-1 protein in itself results in largely neutral changes in primary plant metabolism, stress defence and immune responses. Finally, we showed that lack of a functional OASTL-A1 results in enhanced disease susceptibility against infection with virulent and non-virulent Pseudomonas syringae pv. tomato DC3000 strains. These results reveal an interaction between the cytosolic OASTL and components of plant immunity.

Authors+Show Affiliations

Institute of Molecular BioSciences, Massey University, Private Bag 11222, Palmerston North, New Zealand.Max-Planck-Institut fuer Molekulare Pflanzenphysiologie, Wissenschaftspark Golm, Am Muehlenberg 1, Potsdam-Golm, 14476, Germany.Centre for Bioenergy Plants Research and Development, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China, and.The New Zealand Institute for Plant & Food Research Limited, Private Bag 11600, Palmerston North, 4442, New Zealand.Max-Planck-Institut fuer Molekulare Pflanzenphysiologie, Wissenschaftspark Golm, Am Muehlenberg 1, Potsdam-Golm, 14476, Germany.Max-Planck-Institut fuer Molekulare Pflanzenphysiologie, Wissenschaftspark Golm, Am Muehlenberg 1, Potsdam-Golm, 14476, Germany.Max-Planck-Institut fuer Molekulare Pflanzenphysiologie, Wissenschaftspark Golm, Am Muehlenberg 1, Potsdam-Golm, 14476, Germany.Max-Planck-Institut fuer Molekulare Pflanzenphysiologie, Wissenschaftspark Golm, Am Muehlenberg 1, Potsdam-Golm, 14476, Germany.Max-Planck-Institut fuer Molekulare Pflanzenphysiologie, Wissenschaftspark Golm, Am Muehlenberg 1, Potsdam-Golm, 14476, Germany.Institute of Molecular BioSciences, Massey University, Private Bag 11222, Palmerston North, New Zealand.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22974487

Citation

Tahir, Jibran, et al. "Activation of R-mediated Innate Immunity and Disease Susceptibility Is Affected By Mutations in a Cytosolic O-acetylserine (thiol) Lyase in Arabidopsis." The Plant Journal : for Cell and Molecular Biology, vol. 73, no. 1, 2013, pp. 118-30.
Tahir J, Watanabe M, Jing HC, et al. Activation of R-mediated innate immunity and disease susceptibility is affected by mutations in a cytosolic O-acetylserine (thiol) lyase in Arabidopsis. Plant J. 2013;73(1):118-30.
Tahir, J., Watanabe, M., Jing, H. C., Hunter, D. A., Tohge, T., Nunes-Nesi, A., Brotman, Y., Fernie, A. R., Hoefgen, R., & Dijkwel, P. P. (2013). Activation of R-mediated innate immunity and disease susceptibility is affected by mutations in a cytosolic O-acetylserine (thiol) lyase in Arabidopsis. The Plant Journal : for Cell and Molecular Biology, 73(1), 118-30. https://doi.org/10.1111/tpj.12021
Tahir J, et al. Activation of R-mediated Innate Immunity and Disease Susceptibility Is Affected By Mutations in a Cytosolic O-acetylserine (thiol) Lyase in Arabidopsis. Plant J. 2013;73(1):118-30. PubMed PMID: 22974487.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of R-mediated innate immunity and disease susceptibility is affected by mutations in a cytosolic O-acetylserine (thiol) lyase in Arabidopsis. AU - Tahir,Jibran, AU - Watanabe,Mutsumi, AU - Jing,Hai-Chun, AU - Hunter,Donald A, AU - Tohge,Takayuki, AU - Nunes-Nesi,Adriano, AU - Brotman,Yariv, AU - Fernie,Alisdair R, AU - Hoefgen,Rainer, AU - Dijkwel,Paul P, Y1 - 2012/11/05/ PY - 2012/06/08/received PY - 2012/09/05/revised PY - 2012/09/10/accepted PY - 2012/9/15/entrez PY - 2012/9/15/pubmed PY - 2016/4/14/medline KW - OASTL-A1 KW - RPP1 KW - auto-necrosis KW - cysteine biosynthesis KW - disease resistance KW - innate immunity SP - 118 EP - 30 JF - The Plant journal : for cell and molecular biology JO - Plant J. VL - 73 IS - 1 N2 - O-acetylserine (thiol) lyases (OASTLs) are evolutionarily conserved proteins among many prokaryotes and eukaryotes that perform sulfur acquisition and synthesis of cysteine. A mutation in the cytosolic OASTL-A1 protein ONSET OF LEAF DEATH3 (OLD3) was previously shown to reduce the OASTL activity of the old3-1 protein in vitro and cause auto-necrosis in specific Arabidopsis accessions. Here we investigated why a mutation in this protein causes auto-necrosis in some but not other accessions. The auto-necrosis was found to depend on Recognition of Peronospora Parasitica 1 (RPP1)-like disease resistance R gene(s) from an evolutionarily divergent R gene cluster that is present in Ler-0 but not the reference accession Col-0. RPP1-like gene(s) show a negative epistatic interaction with the old3-1 mutation that is not linked to reduced cysteine biosynthesis. Metabolic profiling and transcriptional analysis further indicate that an effector triggered-like immune response and metabolic disorder are associated with auto-necrosis in old3-1 mutants, probably activated by an RPP1-like gene. However, the old3-1 protein in itself results in largely neutral changes in primary plant metabolism, stress defence and immune responses. Finally, we showed that lack of a functional OASTL-A1 results in enhanced disease susceptibility against infection with virulent and non-virulent Pseudomonas syringae pv. tomato DC3000 strains. These results reveal an interaction between the cytosolic OASTL and components of plant immunity. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/22974487/Activation_of_R_mediated_innate_immunity_and_disease_susceptibility_is_affected_by_mutations_in_a_cytosolic_O_acetylserine__thiol__lyase_in_Arabidopsis_ L2 - https://doi.org/10.1111/tpj.12021 DB - PRIME DP - Unbound Medicine ER -