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Proteomic profiling of dog urine after European adder (Vipera berus berus) envenomation by two-dimensional difference gel electrophoresis.
Toxicon. 2012 Dec 01; 60(7):1228-34.T

Abstract

Between April and September every year, many dogs in Finland are bitten by Vipera berus berus, also known as the European adder, the only venomous snake in the area. Exposure to snake bite venom causes local and systemic symptoms and in severe cases can lead to death. Urine samples were collected from four dogs bitten by V. berus berus and treated in the intensive care unit of the Veterinary Teaching Hospital at the University of Helsinki. The inclusion criteria were a strong suspicion of an adder bite no more than two days before admission and clinical signs of an adder bite. Exclusion criteria were defined as ongoing treatment with glucocorticoids or a known history of liver or kidney diseases. Six privately owned, healthy dogs were obtained as controls. Samples were subjected to 2D-DIGE analysis. Image analysis was performed with DeCyder 7.0 2D software, and protein spots demonstrating a minimum 1.5-fold difference in average spot volume ratios between envenomed and control dogs with a Student's t-test p-value of less than 0.05 were picked and identified using LC-MS/MS. In 2D-DIGE analysis, seven proteins were significantly (p < 0.05) over-expressed in the urine of dogs bitten by V. berus berus compared to the control group. From these, five proteins were identified: beta-2-microglobulin (b2MG), alpha-1-antitrypsin (AAT), albumin, fetuin-B and superoxide dismutase (SOD1). Results indicate that envenomation by V. berus berus alter the urinary protein profile in dogs.

Authors+Show Affiliations

Pharmacology and Toxicology Section, Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57, 00014 Helsinki, Finland. mari.palviainen@helsinki.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22975087

Citation

Palviainen, Mari, et al. "Proteomic Profiling of Dog Urine After European Adder (Vipera Berus Berus) Envenomation By Two-dimensional Difference Gel Electrophoresis." Toxicon : Official Journal of the International Society On Toxinology, vol. 60, no. 7, 2012, pp. 1228-34.
Palviainen M, Raekallio M, Vainionpää M, et al. Proteomic profiling of dog urine after European adder (Vipera berus berus) envenomation by two-dimensional difference gel electrophoresis. Toxicon. 2012;60(7):1228-34.
Palviainen, M., Raekallio, M., Vainionpää, M., Kosonen, S., & Vainio, O. (2012). Proteomic profiling of dog urine after European adder (Vipera berus berus) envenomation by two-dimensional difference gel electrophoresis. Toxicon : Official Journal of the International Society On Toxinology, 60(7), 1228-34. https://doi.org/10.1016/j.toxicon.2012.08.010
Palviainen M, et al. Proteomic Profiling of Dog Urine After European Adder (Vipera Berus Berus) Envenomation By Two-dimensional Difference Gel Electrophoresis. Toxicon. 2012 Dec 1;60(7):1228-34. PubMed PMID: 22975087.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic profiling of dog urine after European adder (Vipera berus berus) envenomation by two-dimensional difference gel electrophoresis. AU - Palviainen,Mari, AU - Raekallio,Marja, AU - Vainionpää,Mari, AU - Kosonen,Suvi, AU - Vainio,Outi, Y1 - 2012/09/05/ PY - 2012/06/13/received PY - 2012/08/16/revised PY - 2012/08/23/accepted PY - 2012/9/15/entrez PY - 2012/9/15/pubmed PY - 2013/3/9/medline SP - 1228 EP - 34 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 60 IS - 7 N2 - Between April and September every year, many dogs in Finland are bitten by Vipera berus berus, also known as the European adder, the only venomous snake in the area. Exposure to snake bite venom causes local and systemic symptoms and in severe cases can lead to death. Urine samples were collected from four dogs bitten by V. berus berus and treated in the intensive care unit of the Veterinary Teaching Hospital at the University of Helsinki. The inclusion criteria were a strong suspicion of an adder bite no more than two days before admission and clinical signs of an adder bite. Exclusion criteria were defined as ongoing treatment with glucocorticoids or a known history of liver or kidney diseases. Six privately owned, healthy dogs were obtained as controls. Samples were subjected to 2D-DIGE analysis. Image analysis was performed with DeCyder 7.0 2D software, and protein spots demonstrating a minimum 1.5-fold difference in average spot volume ratios between envenomed and control dogs with a Student's t-test p-value of less than 0.05 were picked and identified using LC-MS/MS. In 2D-DIGE analysis, seven proteins were significantly (p < 0.05) over-expressed in the urine of dogs bitten by V. berus berus compared to the control group. From these, five proteins were identified: beta-2-microglobulin (b2MG), alpha-1-antitrypsin (AAT), albumin, fetuin-B and superoxide dismutase (SOD1). Results indicate that envenomation by V. berus berus alter the urinary protein profile in dogs. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/22975087/Proteomic_profiling_of_dog_urine_after_European_adder__Vipera_berus_berus__envenomation_by_two_dimensional_difference_gel_electrophoresis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(12)00723-4 DB - PRIME DP - Unbound Medicine ER -