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Formation of zinc-containing nanoparticles from Zn²⁺ ions in cell culture media: implications for the nanotoxicology of ZnO.
Chem Res Toxicol. 2012 Oct 15; 25(10):2057-66.CR

Abstract

Zinc ions generate a range of poorly soluble Zn-containing nanoparticles when added to commonly used mammalian cell culture media. The formation of these nanoparticles confounds the use of soluble Zn salts as positive controls during cytotoxicity testing of other Zn-containing nanoparticles, such as ZnO. These nanoprecipitates can either be crystalline or amorphous and vary in composition depending upon the concentration of Zn(II) within the medium. The cytotoxicity and immune system response of these nanoparticles in situ are similar to those of 30 nm ZnO nanoparticles. The low residual level of truly soluble Zn species (taken as species passing through a 2 kDa membrane) in cell culture media with serum is insufficient to elicit any appreciable cytotoxicity. These observations highlight the importance of employing appropriate controls when studying ZnO nanoparticle toxicity and suggest a re-evaluation of the conclusions drawn in some previous cytotoxicity studies.

Authors+Show Affiliations

Centre for Green Chemistry and Department of Materials Engineering, Monash University, Clayton, Victoria 3800, Australia. terry.turney@monash.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22978249

Citation

Turney, Terence W., et al. "Formation of Zinc-containing Nanoparticles From Zn²⁺ Ions in Cell Culture Media: Implications for the Nanotoxicology of ZnO." Chemical Research in Toxicology, vol. 25, no. 10, 2012, pp. 2057-66.
Turney TW, Duriska MB, Jayaratne V, et al. Formation of zinc-containing nanoparticles from Zn²⁺ ions in cell culture media: implications for the nanotoxicology of ZnO. Chem Res Toxicol. 2012;25(10):2057-66.
Turney, T. W., Duriska, M. B., Jayaratne, V., Elbaz, A., O'Keefe, S. J., Hastings, A. S., Piva, T. J., Wright, P. F., & Feltis, B. N. (2012). Formation of zinc-containing nanoparticles from Zn²⁺ ions in cell culture media: implications for the nanotoxicology of ZnO. Chemical Research in Toxicology, 25(10), 2057-66. https://doi.org/10.1021/tx300241q
Turney TW, et al. Formation of Zinc-containing Nanoparticles From Zn²⁺ Ions in Cell Culture Media: Implications for the Nanotoxicology of ZnO. Chem Res Toxicol. 2012 Oct 15;25(10):2057-66. PubMed PMID: 22978249.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Formation of zinc-containing nanoparticles from Zn²⁺ ions in cell culture media: implications for the nanotoxicology of ZnO. AU - Turney,Terence W, AU - Duriska,Martin B, AU - Jayaratne,Vidura, AU - Elbaz,Abdulkareem, AU - O'Keefe,Sean J, AU - Hastings,Andrew S, AU - Piva,Terrence J, AU - Wright,Paul F A, AU - Feltis,Bryce N, Y1 - 2012/09/27/ PY - 2012/9/18/entrez PY - 2012/9/18/pubmed PY - 2013/3/8/medline SP - 2057 EP - 66 JF - Chemical research in toxicology JO - Chem Res Toxicol VL - 25 IS - 10 N2 - Zinc ions generate a range of poorly soluble Zn-containing nanoparticles when added to commonly used mammalian cell culture media. The formation of these nanoparticles confounds the use of soluble Zn salts as positive controls during cytotoxicity testing of other Zn-containing nanoparticles, such as ZnO. These nanoprecipitates can either be crystalline or amorphous and vary in composition depending upon the concentration of Zn(II) within the medium. The cytotoxicity and immune system response of these nanoparticles in situ are similar to those of 30 nm ZnO nanoparticles. The low residual level of truly soluble Zn species (taken as species passing through a 2 kDa membrane) in cell culture media with serum is insufficient to elicit any appreciable cytotoxicity. These observations highlight the importance of employing appropriate controls when studying ZnO nanoparticle toxicity and suggest a re-evaluation of the conclusions drawn in some previous cytotoxicity studies. SN - 1520-5010 UR - https://www.unboundmedicine.com/medline/citation/22978249/Formation_of_zinc_containing_nanoparticles_from_Zn²⁺_ions_in_cell_culture_media:_implications_for_the_nanotoxicology_of_ZnO_ L2 - https://doi.org/10.1021/tx300241q DB - PRIME DP - Unbound Medicine ER -