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Formation of zinc-containing nanoparticles from Zn²⁺ ions in cell culture media: implications for the nanotoxicology of ZnO.
Chem Res Toxicol. 2012 Oct 15; 25(10):2057-66.CR

Abstract

Zinc ions generate a range of poorly soluble Zn-containing nanoparticles when added to commonly used mammalian cell culture media. The formation of these nanoparticles confounds the use of soluble Zn salts as positive controls during cytotoxicity testing of other Zn-containing nanoparticles, such as ZnO. These nanoprecipitates can either be crystalline or amorphous and vary in composition depending upon the concentration of Zn(II) within the medium. The cytotoxicity and immune system response of these nanoparticles in situ are similar to those of 30 nm ZnO nanoparticles. The low residual level of truly soluble Zn species (taken as species passing through a 2 kDa membrane) in cell culture media with serum is insufficient to elicit any appreciable cytotoxicity. These observations highlight the importance of employing appropriate controls when studying ZnO nanoparticle toxicity and suggest a re-evaluation of the conclusions drawn in some previous cytotoxicity studies.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Turney TW
Centre for Green Chemistry and Department of Materials Engineering, Monash University, Clayton, Victoria 3800, Australia. terry.turney@monash.edu
Duriska MB
No affiliation info available
Jayaratne V
No affiliation info available
Elbaz A
No affiliation info available
O'Keefe SJ
No affiliation info available
Hastings AS
No affiliation info available
Piva TJ
No affiliation info available
Wright PF
No affiliation info available
Feltis BN
No affiliation info available

MeSH

AnimalsCations, DivalentCell LineCell SurvivalMast CellsMiceNanoparticlesSolubilityZincZinc Oxide

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22978249

Citation

Turney, Terence W., et al. "Formation of Zinc-containing Nanoparticles From Zn²⁺ Ions in Cell Culture Media: Implications for the Nanotoxicology of ZnO." Chemical Research in Toxicology, vol. 25, no. 10, 2012, pp. 2057-66.
Turney TW, Duriska MB, Jayaratne V, et al. Formation of zinc-containing nanoparticles from Zn²⁺ ions in cell culture media: implications for the nanotoxicology of ZnO. Chem Res Toxicol. 2012;25(10):2057-66.
Turney, T. W., Duriska, M. B., Jayaratne, V., Elbaz, A., O'Keefe, S. J., Hastings, A. S., Piva, T. J., Wright, P. F., & Feltis, B. N. (2012). Formation of zinc-containing nanoparticles from Zn²⁺ ions in cell culture media: implications for the nanotoxicology of ZnO. Chemical Research in Toxicology, 25(10), 2057-66. https://doi.org/10.1021/tx300241q
Turney TW, et al. Formation of Zinc-containing Nanoparticles From Zn²⁺ Ions in Cell Culture Media: Implications for the Nanotoxicology of ZnO. Chem Res Toxicol. 2012 Oct 15;25(10):2057-66. PubMed PMID: 22978249.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Formation of zinc-containing nanoparticles from Zn²⁺ ions in cell culture media: implications for the nanotoxicology of ZnO. AU - Turney,Terence W, AU - Duriska,Martin B, AU - Jayaratne,Vidura, AU - Elbaz,Abdulkareem, AU - O'Keefe,Sean J, AU - Hastings,Andrew S, AU - Piva,Terrence J, AU - Wright,Paul F A, AU - Feltis,Bryce N, Y1 - 2012/09/27/ PY - 2012/9/18/entrez PY - 2012/9/18/pubmed PY - 2013/3/8/medline SP - 2057 EP - 66 JF - Chemical research in toxicology JO - Chem Res Toxicol VL - 25 IS - 10 N2 - Zinc ions generate a range of poorly soluble Zn-containing nanoparticles when added to commonly used mammalian cell culture media. The formation of these nanoparticles confounds the use of soluble Zn salts as positive controls during cytotoxicity testing of other Zn-containing nanoparticles, such as ZnO. These nanoprecipitates can either be crystalline or amorphous and vary in composition depending upon the concentration of Zn(II) within the medium. The cytotoxicity and immune system response of these nanoparticles in situ are similar to those of 30 nm ZnO nanoparticles. The low residual level of truly soluble Zn species (taken as species passing through a 2 kDa membrane) in cell culture media with serum is insufficient to elicit any appreciable cytotoxicity. These observations highlight the importance of employing appropriate controls when studying ZnO nanoparticle toxicity and suggest a re-evaluation of the conclusions drawn in some previous cytotoxicity studies. SN - 1520-5010 UR - https://www.unboundmedicine.com/medline/citation/22978249/Formation_of_zinc_containing_nanoparticles_from_Zn²⁺_ions_in_cell_culture_media:_implications_for_the_nanotoxicology_of_ZnO_ DB - PRIME DP - Unbound Medicine ER -