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Detection of odorant molecules via surface acoustic wave biosensor array based on odorant-binding proteins.
Biosens Bioelectron. 2013 Mar 15; 41:328-34.BB

Abstract

In this paper, we present an array of biosensors for vapour phase detection of odorant molecules based on surface acoustic wave (SAW) resonators coated with odorant-binding proteins (OBPs). For the first time, the sensing capabilities of three different OBPs, as sensitive layers for SAW devices, are studied and compared. The SAW biosensor array is composed of three SAW devices coated by the droplet method with the wild-type OBP from cow (wtbOBP), a double mutant of the OBP from cow (dmbOBP) and the wild-type OBP from pig (wtpOBP). An uncoated device is used to compensate the variations of the environmental parameters. The SAW devices consist of two-port resonators fabricated on quartz (ST-cut, x propagation) with electrodes made of aluminium covered with a thin gold film (2 nm thick). The obtained surface densities of OBP layers are between 1.18×10(-6) kg/m(2) and 2.31×10(-6) kg/m(2) and were calculated measuring the resonant frequency shift of the SAW devices after the coating. The SAW biosensor array was tested in nitrogen upon exposure to vapours of R-(-)-1-octen-3-ol (octenol), in the range of concentration between 13 and 61 ppm, and R-(-)-carvone (carvone), in the range between 9 and 80 ppm. The highest sensitivity for detection of octenol (25.9 Hz/ppm) was obtained using the wtpOBP-based SAW biosensor, while the highest sensitivity for detection of carvone (9.2 Hz/ppm) was obtained using the dmbOBP-based SAW biosensor.

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Authors+Show Affiliations

Di Pietrantonio F
Institute of Acoustics and Sensors O.M.Corbino, National Research Council of Italy, Via del Fosso del Cavaliere 100, 00133 Rome, Italy. fabio.dp@idasc.cnr.it
Cannatà D
No affiliation info available
Benetti M
No affiliation info available
Verona E
No affiliation info available
Varriale A
No affiliation info available
Staiano M
No affiliation info available
D'Auria S
No affiliation info available

MeSH

Biosensing TechniquesCarrier ProteinsEquipment DesignEquipment Failure AnalysisGasesMicro-Electrical-Mechanical SystemsMicroarray AnalysisOdorantsProtein Interaction MappingReproducibility of ResultsSensitivity and SpecificitySound

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22981410

Citation

Di Pietrantonio, F, et al. "Detection of Odorant Molecules Via Surface Acoustic Wave Biosensor Array Based On Odorant-binding Proteins." Biosensors & Bioelectronics, vol. 41, 2013, pp. 328-34.
Di Pietrantonio F, Cannatà D, Benetti M, et al. Detection of odorant molecules via surface acoustic wave biosensor array based on odorant-binding proteins. Biosens Bioelectron. 2013;41:328-34.
Di Pietrantonio, F., Cannatà, D., Benetti, M., Verona, E., Varriale, A., Staiano, M., & D'Auria, S. (2013). Detection of odorant molecules via surface acoustic wave biosensor array based on odorant-binding proteins. Biosensors & Bioelectronics, 41, 328-34. https://doi.org/10.1016/j.bios.2012.08.046
Di Pietrantonio F, et al. Detection of Odorant Molecules Via Surface Acoustic Wave Biosensor Array Based On Odorant-binding Proteins. Biosens Bioelectron. 2013 Mar 15;41:328-34. PubMed PMID: 22981410.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of odorant molecules via surface acoustic wave biosensor array based on odorant-binding proteins. AU - Di Pietrantonio,F, AU - Cannatà,D, AU - Benetti,M, AU - Verona,E, AU - Varriale,A, AU - Staiano,M, AU - D'Auria,S, Y1 - 2012/08/29/ PY - 2012/07/26/received PY - 2012/08/21/accepted PY - 2012/9/18/entrez PY - 2012/9/18/pubmed PY - 2013/6/1/medline SP - 328 EP - 34 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 41 N2 - In this paper, we present an array of biosensors for vapour phase detection of odorant molecules based on surface acoustic wave (SAW) resonators coated with odorant-binding proteins (OBPs). For the first time, the sensing capabilities of three different OBPs, as sensitive layers for SAW devices, are studied and compared. The SAW biosensor array is composed of three SAW devices coated by the droplet method with the wild-type OBP from cow (wtbOBP), a double mutant of the OBP from cow (dmbOBP) and the wild-type OBP from pig (wtpOBP). An uncoated device is used to compensate the variations of the environmental parameters. The SAW devices consist of two-port resonators fabricated on quartz (ST-cut, x propagation) with electrodes made of aluminium covered with a thin gold film (2 nm thick). The obtained surface densities of OBP layers are between 1.18×10(-6) kg/m(2) and 2.31×10(-6) kg/m(2) and were calculated measuring the resonant frequency shift of the SAW devices after the coating. The SAW biosensor array was tested in nitrogen upon exposure to vapours of R-(-)-1-octen-3-ol (octenol), in the range of concentration between 13 and 61 ppm, and R-(-)-carvone (carvone), in the range between 9 and 80 ppm. The highest sensitivity for detection of octenol (25.9 Hz/ppm) was obtained using the wtpOBP-based SAW biosensor, while the highest sensitivity for detection of carvone (9.2 Hz/ppm) was obtained using the dmbOBP-based SAW biosensor. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/22981410/Detection_of_odorant_molecules_via_surface_acoustic_wave_biosensor_array_based_on_odorant_binding_proteins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(12)00570-2 DB - PRIME DP - Unbound Medicine ER -