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Deficient antiviral immune responses in childhood: distinct roles of atopy and asthma.
J Allergy Clin Immunol 2012; 130(6):1307-14JA

Abstract

BACKGROUND

Impaired immune response to viral infections in atopic asthmatic patients has been recently reported and debated. Whether this condition is present in childhood and whether it is affected by atopy per se deserves further investigation.

OBJECTIVE

We sought to investigate airway interferon production in response to rhinovirus infection in children who are asthmatic, atopic, or both and its correlation with the airway inflammatory profile.

METHODS

Bronchial biopsy specimens and epithelial cells were obtained from 47 children (mean age, 5 ± 0.5 years) undergoing bronchoscopy. The study population included asthmatic children who were either atopic or nonatopic, atopic children without asthma, and children without atopy or asthma. Rhinovirus type 16 induction of IFN-λ and IFN-β mRNA and protein levels was assessed in bronchial epithelial cell cultures. The immunoinflammatory profile was evaluated by means of immunohistochemistry in bronchial biopsy specimens.

RESULTS

Rhinovirus type 16-induced interferon production was significantly reduced in atopic asthmatic, nonatopic asthmatic, and atopic nonasthmatic children compared with that seen in nonatopic nonasthmatic children (all P < .05). Increased rhinovirus viral RNA levels paralleled this deficient interferon induction. Additionally, IFN-λ and IFN-β induction correlated inversely with the airway T(H)2 immunopathologic profile (eosinophilia and IL-4 positivity: P < .05 and r = -0.38 and P < .05 and r = -0.58, respectively) and with epithelial damage (P < .05 and r = -0.55). Furthermore, total serum IgE levels correlated negatively with rhinovirus-induced IFN-λ mRNA levels (P < .05 and r = -0.41) and positively with rhinovirus viral RNA levels (P < .05 and r = 0.44).

CONCLUSIONS

Deficient interferon responses to rhinovirus infection are present in childhood in asthmatic subjects irrespective of their atopic status and in atopic patients without asthma. These findings suggest that deficient immune responses to viral infections are not limited to patients with atopic asthma but are present in those with other T(H)2-oriented conditions.

Authors+Show Affiliations

Department of Cardiac, Thoracic and Vascular Sciences, Section of Respiratory Diseases, University of Padova, Padua, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22981791

Citation

Baraldo, Simonetta, et al. "Deficient Antiviral Immune Responses in Childhood: Distinct Roles of Atopy and Asthma." The Journal of Allergy and Clinical Immunology, vol. 130, no. 6, 2012, pp. 1307-14.
Baraldo S, Contoli M, Bazzan E, et al. Deficient antiviral immune responses in childhood: distinct roles of atopy and asthma. J Allergy Clin Immunol. 2012;130(6):1307-14.
Baraldo, S., Contoli, M., Bazzan, E., Turato, G., Padovani, A., Marku, B., ... Papi, A. (2012). Deficient antiviral immune responses in childhood: distinct roles of atopy and asthma. The Journal of Allergy and Clinical Immunology, 130(6), pp. 1307-14. doi:10.1016/j.jaci.2012.08.005.
Baraldo S, et al. Deficient Antiviral Immune Responses in Childhood: Distinct Roles of Atopy and Asthma. J Allergy Clin Immunol. 2012;130(6):1307-14. PubMed PMID: 22981791.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deficient antiviral immune responses in childhood: distinct roles of atopy and asthma. AU - Baraldo,Simonetta, AU - Contoli,Marco, AU - Bazzan,Erica, AU - Turato,Graziella, AU - Padovani,Anna, AU - Marku,Brunilda, AU - Calabrese,Fiorella, AU - Caramori,Gaetano, AU - Ballarin,Andrea, AU - Snijders,Deborah, AU - Barbato,Angelo, AU - Saetta,Marina, AU - Papi,Alberto, Y1 - 2012/09/13/ PY - 2011/12/23/received PY - 2012/08/01/revised PY - 2012/08/02/accepted PY - 2012/9/18/entrez PY - 2012/9/18/pubmed PY - 2013/3/1/medline SP - 1307 EP - 14 JF - The Journal of allergy and clinical immunology JO - J. Allergy Clin. Immunol. VL - 130 IS - 6 N2 - BACKGROUND: Impaired immune response to viral infections in atopic asthmatic patients has been recently reported and debated. Whether this condition is present in childhood and whether it is affected by atopy per se deserves further investigation. OBJECTIVE: We sought to investigate airway interferon production in response to rhinovirus infection in children who are asthmatic, atopic, or both and its correlation with the airway inflammatory profile. METHODS: Bronchial biopsy specimens and epithelial cells were obtained from 47 children (mean age, 5 ± 0.5 years) undergoing bronchoscopy. The study population included asthmatic children who were either atopic or nonatopic, atopic children without asthma, and children without atopy or asthma. Rhinovirus type 16 induction of IFN-λ and IFN-β mRNA and protein levels was assessed in bronchial epithelial cell cultures. The immunoinflammatory profile was evaluated by means of immunohistochemistry in bronchial biopsy specimens. RESULTS: Rhinovirus type 16-induced interferon production was significantly reduced in atopic asthmatic, nonatopic asthmatic, and atopic nonasthmatic children compared with that seen in nonatopic nonasthmatic children (all P < .05). Increased rhinovirus viral RNA levels paralleled this deficient interferon induction. Additionally, IFN-λ and IFN-β induction correlated inversely with the airway T(H)2 immunopathologic profile (eosinophilia and IL-4 positivity: P < .05 and r = -0.38 and P < .05 and r = -0.58, respectively) and with epithelial damage (P < .05 and r = -0.55). Furthermore, total serum IgE levels correlated negatively with rhinovirus-induced IFN-λ mRNA levels (P < .05 and r = -0.41) and positively with rhinovirus viral RNA levels (P < .05 and r = 0.44). CONCLUSIONS: Deficient interferon responses to rhinovirus infection are present in childhood in asthmatic subjects irrespective of their atopic status and in atopic patients without asthma. These findings suggest that deficient immune responses to viral infections are not limited to patients with atopic asthma but are present in those with other T(H)2-oriented conditions. SN - 1097-6825 UR - https://www.unboundmedicine.com/medline/citation/22981791/Deficient_antiviral_immune_responses_in_childhood:_distinct_roles_of_atopy_and_asthma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(12)01291-2 DB - PRIME DP - Unbound Medicine ER -