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HPLC-ESI-QTOF-MS as a powerful analytical tool for characterising phenolic compounds in olive-leaf extracts.
Phytochem Anal. 2013 May-Jun; 24(3):213-23.PA

Abstract

INTRODUCTION

Olea europaea L. leaves may be considered a cheap, easily available natural source of phenolic compounds. In a previous study we evaluated the possibility of obtaining bioactive phenolic compounds from olive leaves by pressurised liquid extraction (PLE) for their use as natural anti-oxidants. The alimentary use of these kinds of extract makes comprehensive knowledge of their composition essential.

OBJECTIVE

To undertake a comprehensive characterisation of two olive-leaf extracts obtained by PLE using high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS).

METHOD

Olive leaves were extracted by PLE using ethanol and water as extraction solvents at 150°C and 200°C respectively. Separation was carried out in a HPLC system equipped with a C₁₈-column working in a gradient elution programme coupled to ESI-QTOF-MS operating in negative ion mode.

RESULTS

This analytical platform was able to detect 48 compounds and tentatively identify 31 different phenolic compounds in these extracts, including secoiridoids, simple phenols, flavonoids, cinnamic-acid derivatives and benzoic acids. Lucidumoside C was also identified for the first time in olive leaves.

CONCLUSION

The coupling of HPLC-ESI-QTOF-MS led to the in-depth characterisation of the olive-leaf extracts on the basis of mass accuracy, true isotopic pattern and tandem mass spectrometry (MS/MS) spectra. We may conclude therefore that this analytical tool is very valuable in the study of phenolic compounds in plant matrices.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Quirantes-Piné R
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, c/ Fuentenueva s/n, 18071 Granada, Spain.
Lozano-Sánchez J
No affiliation info available
Herrero M
No affiliation info available
Ibáñez E
No affiliation info available
Segura-Carretero A
No affiliation info available
Fernández-Gutiérrez A
No affiliation info available

MeSH

BenzoatesChromatography, High Pressure LiquidCinnamatesEquipment DesignFlavonoidsIridoidsLiquid-Liquid ExtractionOleaPhenolsPlant ExtractsPlant LeavesPolyphenolsSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22987739

Citation

Quirantes-Piné, Rosa, et al. "HPLC-ESI-QTOF-MS as a Powerful Analytical Tool for Characterising Phenolic Compounds in Olive-leaf Extracts." Phytochemical Analysis : PCA, vol. 24, no. 3, 2013, pp. 213-23.
Quirantes-Piné R, Lozano-Sánchez J, Herrero M, et al. HPLC-ESI-QTOF-MS as a powerful analytical tool for characterising phenolic compounds in olive-leaf extracts. Phytochem Anal. 2013;24(3):213-23.
Quirantes-Piné, R., Lozano-Sánchez, J., Herrero, M., Ibáñez, E., Segura-Carretero, A., & Fernández-Gutiérrez, A. (2013). HPLC-ESI-QTOF-MS as a powerful analytical tool for characterising phenolic compounds in olive-leaf extracts. Phytochemical Analysis : PCA, 24(3), 213-23. https://doi.org/10.1002/pca.2401
Quirantes-Piné R, et al. HPLC-ESI-QTOF-MS as a Powerful Analytical Tool for Characterising Phenolic Compounds in Olive-leaf Extracts. Phytochem Anal. 2013 May-Jun;24(3):213-23. PubMed PMID: 22987739.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPLC-ESI-QTOF-MS as a powerful analytical tool for characterising phenolic compounds in olive-leaf extracts. AU - Quirantes-Piné,Rosa, AU - Lozano-Sánchez,Jesús, AU - Herrero,Miguel, AU - Ibáñez,Elena, AU - Segura-Carretero,Antonio, AU - Fernández-Gutiérrez,Alberto, Y1 - 2012/09/15/ PY - 2012/02/09/received PY - 2012/06/30/revised PY - 2012/08/14/accepted PY - 2012/9/19/entrez PY - 2012/9/19/pubmed PY - 2014/1/9/medline SP - 213 EP - 23 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 24 IS - 3 N2 - INTRODUCTION: Olea europaea L. leaves may be considered a cheap, easily available natural source of phenolic compounds. In a previous study we evaluated the possibility of obtaining bioactive phenolic compounds from olive leaves by pressurised liquid extraction (PLE) for their use as natural anti-oxidants. The alimentary use of these kinds of extract makes comprehensive knowledge of their composition essential. OBJECTIVE: To undertake a comprehensive characterisation of two olive-leaf extracts obtained by PLE using high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS). METHOD: Olive leaves were extracted by PLE using ethanol and water as extraction solvents at 150°C and 200°C respectively. Separation was carried out in a HPLC system equipped with a C₁₈-column working in a gradient elution programme coupled to ESI-QTOF-MS operating in negative ion mode. RESULTS: This analytical platform was able to detect 48 compounds and tentatively identify 31 different phenolic compounds in these extracts, including secoiridoids, simple phenols, flavonoids, cinnamic-acid derivatives and benzoic acids. Lucidumoside C was also identified for the first time in olive leaves. CONCLUSION: The coupling of HPLC-ESI-QTOF-MS led to the in-depth characterisation of the olive-leaf extracts on the basis of mass accuracy, true isotopic pattern and tandem mass spectrometry (MS/MS) spectra. We may conclude therefore that this analytical tool is very valuable in the study of phenolic compounds in plant matrices. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/22987739/HPLC_ESI_QTOF_MS_as_a_powerful_analytical_tool_for_characterising_phenolic_compounds_in_olive_leaf_extracts_ L2 - https://doi.org/10.1002/pca.2401 DB - PRIME DP - Unbound Medicine ER -