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Antioxidant profile of Trifolium pratense L.
Molecules. 2012 Sep 18; 17(9):11156-72.M

Abstract

In order to examine the antioxidant properties of five different extracts of Trifolium pratense L. (Leguminosae) leaves, various assays which measure free radical scavenging ability were carried out: 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, superoxide anion and nitric oxide radical scavenger capacity tests and lipid peroxidation assay. In all of the tests, only the H2O and (to some extent) the EtOAc extracts showed a potent antioxidant effect compared with BHT and BHA, well-known synthetic antioxidants. In addition, in vivo experiments were conducted with antioxidant systems (activities of GSHPx, GSHR, Px, CAT, XOD, GSH content and intensity of LPx) in liver homogenate and blood of mice after their treatment with extracts of T. pratense leaves, or in combination with CCl4. Besides, in the extracts examined the total phenolic and flavonoid amounts were also determined, together with presence of the selected flavonoids: quercetin, luteolin, apigenin, naringenin and kaempferol, which were studied using a HPLC-DAD technique. HPLC-DAD analysis showed a noticeable content of natural products according to which the examined Trifolium pratense species could well be regarded as a promising new source of bioactive natural compounds, which can be used both as a food supplement and a remedy.

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Authors+Show Affiliations

Kaurinovic B
Department of Chemistry, Faculty of Sciences, Trg Dositeja Obradovica 3, Novi Sad 21000, Serbia. biljana.kaurinovic@dh.uns.ac.rs
Popovic M
No affiliation info available
Vlaisavljevic S
No affiliation info available
Schwartsova H
No affiliation info available
Vojinovic-Miloradov M
No affiliation info available

MeSH

AnimalsAntioxidantsBiphenyl CompoundsFemaleFlavonoidsFree Radical ScavengersHydroxyl RadicalLipid PeroxidationLiverMaleMiceMice, Inbred BALB CNitric OxidePicratesPlant ExtractsPlant LeavesSuperoxidesTrifolium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22990457

Citation

Kaurinovic, Biljana, et al. "Antioxidant Profile of Trifolium Pratense L." Molecules (Basel, Switzerland), vol. 17, no. 9, 2012, pp. 11156-72.
Kaurinovic B, Popovic M, Vlaisavljevic S, et al. Antioxidant profile of Trifolium pratense L. Molecules. 2012;17(9):11156-72.
Kaurinovic, B., Popovic, M., Vlaisavljevic, S., Schwartsova, H., & Vojinovic-Miloradov, M. (2012). Antioxidant profile of Trifolium pratense L. Molecules (Basel, Switzerland), 17(9), 11156-72.
Kaurinovic B, et al. Antioxidant Profile of Trifolium Pratense L. Molecules. 2012 Sep 18;17(9):11156-72. PubMed PMID: 22990457.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant profile of Trifolium pratense L. AU - Kaurinovic,Biljana, AU - Popovic,Mira, AU - Vlaisavljevic,Sanja, AU - Schwartsova,Heidy, AU - Vojinovic-Miloradov,Mirjana, Y1 - 2012/09/18/ PY - 2012/08/20/received PY - 2012/09/05/revised PY - 2012/09/07/accepted PY - 2012/9/20/entrez PY - 2012/9/20/pubmed PY - 2013/3/8/medline SP - 11156 EP - 72 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 17 IS - 9 N2 - In order to examine the antioxidant properties of five different extracts of Trifolium pratense L. (Leguminosae) leaves, various assays which measure free radical scavenging ability were carried out: 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, superoxide anion and nitric oxide radical scavenger capacity tests and lipid peroxidation assay. In all of the tests, only the H2O and (to some extent) the EtOAc extracts showed a potent antioxidant effect compared with BHT and BHA, well-known synthetic antioxidants. In addition, in vivo experiments were conducted with antioxidant systems (activities of GSHPx, GSHR, Px, CAT, XOD, GSH content and intensity of LPx) in liver homogenate and blood of mice after their treatment with extracts of T. pratense leaves, or in combination with CCl4. Besides, in the extracts examined the total phenolic and flavonoid amounts were also determined, together with presence of the selected flavonoids: quercetin, luteolin, apigenin, naringenin and kaempferol, which were studied using a HPLC-DAD technique. HPLC-DAD analysis showed a noticeable content of natural products according to which the examined Trifolium pratense species could well be regarded as a promising new source of bioactive natural compounds, which can be used both as a food supplement and a remedy. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/22990457/Antioxidant_profile_of_Trifolium_pratense_L_ L2 - https://www.mdpi.com/resolver?pii=molecules170911156 DB - PRIME DP - Unbound Medicine ER -