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Biomarkers of HPV in head and neck squamous cell carcinoma.
Cancer Res. 2012 Oct 01; 72(19):5004-13.CR

Abstract

Human papillomavirus (HPV) is an accepted cause of head and neck squamous cell carcinoma (HNSCC), and patients with HPV-associated HNSCC have a favorable prognosis. Currently, there is no general guidance on the most appropriate biomarkers for clinical assessment of HPV in these malignancies. We compared PCR-based and serologic HPV assays, as well as p16 immunohistochemistry, individually and in combination in a single population-based study to assess their associations with overall survival among patients with HNSCC, and thus their potential value as biomarkers. HPV16 serology was determined for 488 patients; immunohistochemical detection of p16 expression in tumors was conducted in a subset of 233 cases, and PCR-based methods to assess the presence of HPV16 DNA in a subset of 179 cases of tumors. Considering each biomarker individually in the subset of patients studied for all endpoints, seropositivity for the E6 and E7 proteins was significantly associated with enhanced all-cause survival in oropharyngeal disease [HR(E6/E7+) = 0.1, 95% confidence interval (CI) = 0.02-0.3]. Neither the presence of HPV16 DNA nor p16 immunostaining was associated with significant enhanced overall survival in oropharyngeal disease (HR(DNA) = 0.9, 95% CI = 0.3-2.9; HR(p16) = 0.3, 95% CI = 0.1-1.1). However, the combination of HPV-positive DNA and E6 or E7 serology was associated with enhanced overall survival in oropharyngeal disease (HR(DNA+/E6/E7+) = 0.1, 95% CI = 0.02-1.0), whereas E6/E7 seronegative patients with evidence of HPV in tumor DNA did not show any evidence of favorable survival (HR(DNA+/E6-/E7-) = 3.4, 95% CI = 0.6-18.1). Furthermore, patients with p16 staining and E6 or E7 seropositivity had favorable survival from oropharyngeal disease (HR(p16+/E6/E7+) = 0.1, 95% CI = 0.02-0.4), whereas patients who were p16 positive and E6/E7 seronegative had significantly increased hazard of all causes of death (HR(p16+/E6-/E7-) = 3.1, 95% CI = 1.2-7.7). A stronger association of HPV presence with prognosis (assessed by all-cause survival) is observed when "HPV-associated" HNSCC is defined using tumor status (HPV DNA status or P16) and HPV E6/E7 serology in combination rather using tumor HPV status alone.

Authors+Show Affiliations

Department of Epidemiology, Division of Biology and Medicine, Brown University, Providence, Rhode Island, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22991304

Citation

Liang, Caihua, et al. "Biomarkers of HPV in Head and Neck Squamous Cell Carcinoma." Cancer Research, vol. 72, no. 19, 2012, pp. 5004-13.
Liang C, Marsit CJ, McClean MD, et al. Biomarkers of HPV in head and neck squamous cell carcinoma. Cancer Res. 2012;72(19):5004-13.
Liang, C., Marsit, C. J., McClean, M. D., Nelson, H. H., Christensen, B. C., Haddad, R. I., Clark, J. R., Wein, R. O., Grillone, G. A., Houseman, E. A., Halec, G., Waterboer, T., Pawlita, M., Krane, J. F., & Kelsey, K. T. (2012). Biomarkers of HPV in head and neck squamous cell carcinoma. Cancer Research, 72(19), 5004-13. https://doi.org/10.1158/0008-5472.CAN-11-3277
Liang C, et al. Biomarkers of HPV in Head and Neck Squamous Cell Carcinoma. Cancer Res. 2012 Oct 1;72(19):5004-13. PubMed PMID: 22991304.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biomarkers of HPV in head and neck squamous cell carcinoma. AU - Liang,Caihua, AU - Marsit,Carmen J, AU - McClean,Michael D, AU - Nelson,Heather H, AU - Christensen,Brock C, AU - Haddad,Robert I, AU - Clark,John R, AU - Wein,Richard O, AU - Grillone,Gregory A, AU - Houseman,E Andres, AU - Halec,Gordana, AU - Waterboer,Tim, AU - Pawlita,Michael, AU - Krane,Jeffrey F, AU - Kelsey,Karl T, Y1 - 2012/09/18/ PY - 2012/9/20/entrez PY - 2012/9/20/pubmed PY - 2012/12/22/medline SP - 5004 EP - 13 JF - Cancer research JO - Cancer Res. VL - 72 IS - 19 N2 - Human papillomavirus (HPV) is an accepted cause of head and neck squamous cell carcinoma (HNSCC), and patients with HPV-associated HNSCC have a favorable prognosis. Currently, there is no general guidance on the most appropriate biomarkers for clinical assessment of HPV in these malignancies. We compared PCR-based and serologic HPV assays, as well as p16 immunohistochemistry, individually and in combination in a single population-based study to assess their associations with overall survival among patients with HNSCC, and thus their potential value as biomarkers. HPV16 serology was determined for 488 patients; immunohistochemical detection of p16 expression in tumors was conducted in a subset of 233 cases, and PCR-based methods to assess the presence of HPV16 DNA in a subset of 179 cases of tumors. Considering each biomarker individually in the subset of patients studied for all endpoints, seropositivity for the E6 and E7 proteins was significantly associated with enhanced all-cause survival in oropharyngeal disease [HR(E6/E7+) = 0.1, 95% confidence interval (CI) = 0.02-0.3]. Neither the presence of HPV16 DNA nor p16 immunostaining was associated with significant enhanced overall survival in oropharyngeal disease (HR(DNA) = 0.9, 95% CI = 0.3-2.9; HR(p16) = 0.3, 95% CI = 0.1-1.1). However, the combination of HPV-positive DNA and E6 or E7 serology was associated with enhanced overall survival in oropharyngeal disease (HR(DNA+/E6/E7+) = 0.1, 95% CI = 0.02-1.0), whereas E6/E7 seronegative patients with evidence of HPV in tumor DNA did not show any evidence of favorable survival (HR(DNA+/E6-/E7-) = 3.4, 95% CI = 0.6-18.1). Furthermore, patients with p16 staining and E6 or E7 seropositivity had favorable survival from oropharyngeal disease (HR(p16+/E6/E7+) = 0.1, 95% CI = 0.02-0.4), whereas patients who were p16 positive and E6/E7 seronegative had significantly increased hazard of all causes of death (HR(p16+/E6-/E7-) = 3.1, 95% CI = 1.2-7.7). A stronger association of HPV presence with prognosis (assessed by all-cause survival) is observed when "HPV-associated" HNSCC is defined using tumor status (HPV DNA status or P16) and HPV E6/E7 serology in combination rather using tumor HPV status alone. SN - 1538-7445 UR - https://www.unboundmedicine.com/medline/citation/22991304/Biomarkers_of_HPV_in_head_and_neck_squamous_cell_carcinoma_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=22991304 DB - PRIME DP - Unbound Medicine ER -