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Gibberellin 20-oxidase gene OsGA20ox3 regulates plant stature and disease development in rice.
Mol Plant Microbe Interact 2013; 26(2):227-39MP

Abstract

Gibberellin (GA) 20-oxidase (GA20ox) catalyses consecutive steps of oxidation in the late part of the GA biosynthetic pathway. A T-DNA insertion mutant (17S-14) in rice, with an elongated phenotype, was isolated. Analysis of the flanking sequences of the T-DNA insertion site revealed that an incomplete T-DNA integration resulted in enhanced constitutively expression of downstream OsGA20ox3 in the mutant. The accumulation of bioactive GA(1) and GA(4) were increased in the mutant in comparison with the wild-type plant. Transgenic plants overexpressing OsGA20ox3 showed phenotypes similar to those of the 17S-14 mutant, and the RNA interference (RNAi) lines that had decreased OsGA20ox3 expression exhibited a semidwarf phenotype. Expression of OsGA20ox3 was detected in the leaves and roots of young seedlings, immature panicles, anthers, and pollens, based on β-glucuronidase (GUS) activity staining in transgenic plants expressing the OsGA20ox3 promoter fused to the GUS gene. The OsGA20ox3 RNAi lines showed enhanced resistance against rice pathogens Magnaporthe oryzae (causing rice blast) and Xanthomonas oryzae pv. oryzae (causing bacterial blight) and increased expression of defense-related genes. Conversely, OsGA20ox3-overexpressing plants were more susceptible to these pathogens comparing with the wild-type plants. The susceptibility of wild-type plants to X. oryzae pv. oryzae was increased by exogenous application of GA(3) and decreased by S-3307 treatment. Together, the results provide direct evidence for a critical role of OsGA20ox3 in regulating not only plant stature but also disease resistance in rice.

Authors+Show Affiliations

State Key Laboratory of Agrobiotechnology, China Agricultural University, Yuanmingyan West Road 2, Beijing 100193, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22992000

Citation

Qin, Xue, et al. "Gibberellin 20-oxidase Gene OsGA20ox3 Regulates Plant Stature and Disease Development in Rice." Molecular Plant-microbe Interactions : MPMI, vol. 26, no. 2, 2013, pp. 227-39.
Qin X, Liu JH, Zhao WS, et al. Gibberellin 20-oxidase gene OsGA20ox3 regulates plant stature and disease development in rice. Mol Plant Microbe Interact. 2013;26(2):227-39.
Qin, X., Liu, J. H., Zhao, W. S., Chen, X. J., Guo, Z. J., & Peng, Y. L. (2013). Gibberellin 20-oxidase gene OsGA20ox3 regulates plant stature and disease development in rice. Molecular Plant-microbe Interactions : MPMI, 26(2), pp. 227-39. doi:10.1094/MPMI-05-12-0138-R.
Qin X, et al. Gibberellin 20-oxidase Gene OsGA20ox3 Regulates Plant Stature and Disease Development in Rice. Mol Plant Microbe Interact. 2013;26(2):227-39. PubMed PMID: 22992000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gibberellin 20-oxidase gene OsGA20ox3 regulates plant stature and disease development in rice. AU - Qin,Xue, AU - Liu,Jun Hua, AU - Zhao,Wen Sheng, AU - Chen,Xu Jun, AU - Guo,Ze Jian, AU - Peng,You Liang, PY - 2012/9/21/entrez PY - 2012/9/21/pubmed PY - 2014/6/17/medline SP - 227 EP - 39 JF - Molecular plant-microbe interactions : MPMI JO - Mol. Plant Microbe Interact. VL - 26 IS - 2 N2 - Gibberellin (GA) 20-oxidase (GA20ox) catalyses consecutive steps of oxidation in the late part of the GA biosynthetic pathway. A T-DNA insertion mutant (17S-14) in rice, with an elongated phenotype, was isolated. Analysis of the flanking sequences of the T-DNA insertion site revealed that an incomplete T-DNA integration resulted in enhanced constitutively expression of downstream OsGA20ox3 in the mutant. The accumulation of bioactive GA(1) and GA(4) were increased in the mutant in comparison with the wild-type plant. Transgenic plants overexpressing OsGA20ox3 showed phenotypes similar to those of the 17S-14 mutant, and the RNA interference (RNAi) lines that had decreased OsGA20ox3 expression exhibited a semidwarf phenotype. Expression of OsGA20ox3 was detected in the leaves and roots of young seedlings, immature panicles, anthers, and pollens, based on β-glucuronidase (GUS) activity staining in transgenic plants expressing the OsGA20ox3 promoter fused to the GUS gene. The OsGA20ox3 RNAi lines showed enhanced resistance against rice pathogens Magnaporthe oryzae (causing rice blast) and Xanthomonas oryzae pv. oryzae (causing bacterial blight) and increased expression of defense-related genes. Conversely, OsGA20ox3-overexpressing plants were more susceptible to these pathogens comparing with the wild-type plants. The susceptibility of wild-type plants to X. oryzae pv. oryzae was increased by exogenous application of GA(3) and decreased by S-3307 treatment. Together, the results provide direct evidence for a critical role of OsGA20ox3 in regulating not only plant stature but also disease resistance in rice. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/22992000/Gibberellin_20_oxidase_gene_OsGA20ox3_regulates_plant_stature_and_disease_development_in_rice_ L2 - http://apsjournals.apsnet.org/doi/full/10.1094/MPMI-05-12-0138-R?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -