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Evidence supporting a zoonotic origin of human coronavirus strain NL63.
J Virol. 2012 Dec; 86(23):12816-25.JV

Abstract

The relationship between bats and coronaviruses (CoVs) has received considerable attention since the severe acute respiratory syndrome (SARS)-like CoV was identified in the Chinese horseshoe bat (Rhinolophidae) in 2005. Since then, several bats throughout the world have been shown to shed CoV sequences, and presumably CoVs, in the feces; however, no bat CoVs have been isolated from nature. Moreover, there are very few bat cell lines or reagents available for investigating CoV replication in bat cells or for isolating bat CoVs adapted to specific bat species. Here, we show by molecular clock analysis that alphacoronavirus (α-CoV) sequences derived from the North American tricolored bat (Perimyotis subflavus) are predicted to share common ancestry with human CoV (HCoV)-NL63, with the most recent common ancestor between these viruses occurring approximately 563 to 822 years ago. Further, we developed immortalized bat cell lines from the lungs of this bat species to determine if these cells were capable of supporting infection with HCoVs. While SARS-CoV, mouse-adapted SARS-CoV (MA15), and chimeric SARS-CoVs bearing the spike genes of early human strains replicated inefficiently, HCoV-NL63 replicated for multiple passages in the immortalized lung cells from this bat species. These observations support the hypothesis that human CoVs are capable of establishing zoonotic-reverse zoonotic transmission cycles that may allow some CoVs to readily circulate and exchange genetic material between strains found in bats and other mammals, including humans.

Authors+Show Affiliations

Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, American Recovery and Reinvestment Act
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

22993147

Citation

Huynh, Jeremy, et al. "Evidence Supporting a Zoonotic Origin of Human Coronavirus Strain NL63." Journal of Virology, vol. 86, no. 23, 2012, pp. 12816-25.
Huynh J, Li S, Yount B, et al. Evidence supporting a zoonotic origin of human coronavirus strain NL63. J Virol. 2012;86(23):12816-25.
Huynh, J., Li, S., Yount, B., Smith, A., Sturges, L., Olsen, J. C., Nagel, J., Johnson, J. B., Agnihothram, S., Gates, J. E., Frieman, M. B., Baric, R. S., & Donaldson, E. F. (2012). Evidence supporting a zoonotic origin of human coronavirus strain NL63. Journal of Virology, 86(23), 12816-25. https://doi.org/10.1128/JVI.00906-12
Huynh J, et al. Evidence Supporting a Zoonotic Origin of Human Coronavirus Strain NL63. J Virol. 2012;86(23):12816-25. PubMed PMID: 22993147.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence supporting a zoonotic origin of human coronavirus strain NL63. AU - Huynh,Jeremy, AU - Li,Shimena, AU - Yount,Boyd, AU - Smith,Alexander, AU - Sturges,Leslie, AU - Olsen,John C, AU - Nagel,Juliet, AU - Johnson,Joshua B, AU - Agnihothram,Sudhakar, AU - Gates,J Edward, AU - Frieman,Matthew B, AU - Baric,Ralph S, AU - Donaldson,Eric F, Y1 - 2012/09/19/ PY - 2012/9/21/entrez PY - 2012/9/21/pubmed PY - 2013/1/15/medline SP - 12816 EP - 25 JF - Journal of virology JO - J Virol VL - 86 IS - 23 N2 - The relationship between bats and coronaviruses (CoVs) has received considerable attention since the severe acute respiratory syndrome (SARS)-like CoV was identified in the Chinese horseshoe bat (Rhinolophidae) in 2005. Since then, several bats throughout the world have been shown to shed CoV sequences, and presumably CoVs, in the feces; however, no bat CoVs have been isolated from nature. Moreover, there are very few bat cell lines or reagents available for investigating CoV replication in bat cells or for isolating bat CoVs adapted to specific bat species. Here, we show by molecular clock analysis that alphacoronavirus (α-CoV) sequences derived from the North American tricolored bat (Perimyotis subflavus) are predicted to share common ancestry with human CoV (HCoV)-NL63, with the most recent common ancestor between these viruses occurring approximately 563 to 822 years ago. Further, we developed immortalized bat cell lines from the lungs of this bat species to determine if these cells were capable of supporting infection with HCoVs. While SARS-CoV, mouse-adapted SARS-CoV (MA15), and chimeric SARS-CoVs bearing the spike genes of early human strains replicated inefficiently, HCoV-NL63 replicated for multiple passages in the immortalized lung cells from this bat species. These observations support the hypothesis that human CoVs are capable of establishing zoonotic-reverse zoonotic transmission cycles that may allow some CoVs to readily circulate and exchange genetic material between strains found in bats and other mammals, including humans. SN - 1098-5514 UR - https://www.unboundmedicine.com/medline/citation/22993147/Evidence_supporting_a_zoonotic_origin_of_human_coronavirus_strain_NL63_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=22993147 DB - PRIME DP - Unbound Medicine ER -