Analysis of short-chain aliphatic amines in food and water samples using a near infrared cyanine 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate potassium with CE-LIF detection.
Electrophoresis. 2012 Oct; 33(19-20):3002-7.E

Abstract

Precolumn derivatization of six short-chain aliphatic amines by a near-infrared dye, 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'- disulfonate potassium (MeCy5-OSu), followed by MEKC-CE-LIF detection has been developed as a method for the determination of aliphatic amines in environmental water and food. Optimum derivatization was operated nicely in pH 9.0 borate buffer at 20°C for 30 min. Well separated peaks were observed with a pH 9.5 BGE containing 10 mmol L⁻¹ phosphoric acid, 20 mmol L⁻¹ SDS, and 7% methanol buffered with 1.0 mol L⁻¹ NaOH. The separation procedure was rapidly achieved within 11 min and the matrix interferences could be effectively eliminated. A linear calibration graph was obtained for 5-200 nmol L⁻¹ analytes with a correlation coefficient in the range 0.9933-0.9995 for amines. This method was successfully utilized to determine aliphatic amines in lake, sewage water, and red wine with recoveries ranging from 96.4 to 105% and the RSDs ranging from 0.9 to 2.9%. Near-infrared, LIF-detector-compatible MeCy5-OSu was proved suitable for the accurate, sensitive, and rapid separation and determination of aliphatic amines in water and food samples.

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Authors+Show Affiliations

Fu NN

Key Laboratory of Analytical Chemistry for Biology and Medicine-Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, P. R. China.

Zhang HS

No affiliation info available

Wang H

No affiliation info available

MeSH

AminesCarbocyaninesElectrophoresis, CapillaryFluorescent DyesHydrogen-Ion ConcentrationLakesLinear ModelsMethanolReproducibility of ResultsSensitivity and SpecificitySewageSodium Dodecyl SulfateSpectrometry, FluorescenceWater Pollutants, ChemicalWine

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22996817

Citation

Fu, Ni-Na, et al. "Analysis of Short-chain Aliphatic Amines in Food and Water Samples Using a Near Infrared Cyanine 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate Potassium With CE-LIF Detection." Electrophoresis, vol. 33, no. 19-20, 2012, pp. 3002-7.

Fu NN, Zhang HS, Wang H. Analysis of short-chain aliphatic amines in food and water samples using a near infrared cyanine 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate potassium with CE-LIF detection. Electrophoresis. 2012;33(19-20):3002-7.

Fu, N. N., Zhang, H. S., & Wang, H. (2012). Analysis of short-chain aliphatic amines in food and water samples using a near infrared cyanine 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate potassium with CE-LIF detection. Electrophoresis, 33(19-20), 3002-7. https://doi.org/10.1002/elps.201200237

Fu NN, Zhang HS, Wang H. Analysis of Short-chain Aliphatic Amines in Food and Water Samples Using a Near Infrared Cyanine 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate Potassium With CE-LIF Detection. Electrophoresis. 2012;33(19-20):3002-7. PubMed PMID: 22996817.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Analysis of short-chain aliphatic amines in food and water samples using a near infrared cyanine 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate potassium with CE-LIF detection. AU - Fu,Ni-Na, AU - Zhang,Hua-Shan, AU - Wang,Hong, Y1 - 2012/09/20/ PY - 2012/04/22/received PY - 2012/07/13/revised PY - 2012/07/24/accepted PY - 2012/9/22/entrez PY - 2012/9/22/pubmed PY - 2013/3/5/medline SP - 3002 EP - 7 JF - Electrophoresis JO - Electrophoresis VL - 33 IS - 19-20 N2 - Precolumn derivatization of six short-chain aliphatic amines by a near-infrared dye, 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'- disulfonate potassium (MeCy5-OSu), followed by MEKC-CE-LIF detection has been developed as a method for the determination of aliphatic amines in environmental water and food. Optimum derivatization was operated nicely in pH 9.0 borate buffer at 20°C for 30 min. Well separated peaks were observed with a pH 9.5 BGE containing 10 mmol L⁻¹ phosphoric acid, 20 mmol L⁻¹ SDS, and 7% methanol buffered with 1.0 mol L⁻¹ NaOH. The separation procedure was rapidly achieved within 11 min and the matrix interferences could be effectively eliminated. A linear calibration graph was obtained for 5-200 nmol L⁻¹ analytes with a correlation coefficient in the range 0.9933-0.9995 for amines. This method was successfully utilized to determine aliphatic amines in lake, sewage water, and red wine with recoveries ranging from 96.4 to 105% and the RSDs ranging from 0.9 to 2.9%. Near-infrared, LIF-detector-compatible MeCy5-OSu was proved suitable for the accurate, sensitive, and rapid separation and determination of aliphatic amines in water and food samples. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/22996817/Analysis_of_short_chain_aliphatic_amines_in_food_and_water_samples_using_a_near_infrared_cyanine_1__ε_succinimydyl_hexanoate__1'_methyl_333'3'_tetramethyl_indocarbocyanine_55'_disulfonate_potassium_with_CE_LIF_detection_ L2 - https://doi.org/10.1002/elps.201200237 DB - PRIME DP - Unbound Medicine ER -

Tags

Analysis of short-chain aliphatic amines in food and water samples using a near infrared cyanine 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate potassium with CE-LIF detection.Electrophoresis. 2012 Oct; 33(19-20):3002-7.E