Tags

Type your tag names separated by a space and hit enter

Oxidative stress and nitrosative stress are involved in different stages of proteolytic pulmonary emphysema.
Free Radic Biol Med. 2012 Dec 01; 53(11):1993-2001.FR

Abstract

Our aim was to investigate the role of oxidative stress in elastase-induced pulmonary emphysema. C57BL/6 mice were subjected to pancreatic porcine elastase (PPE) instillation (0.05 or 0.5 U per mouse, i.t.) to induce pulmonary emphysema. Lungs were collected on days 7, 14, and 21 after PPE instillation. The control group was sham injected. Also, mice treated with 1% aminoguanidine (AMG) and inducible NO synthase (iNOS) knockout mice received 0.5 U PPE (i.t.), and lungs were analyzed 21 days after. We performed bronchoalveolar lavage, biochemical analyses of oxidative stress, and lung stereology and morphometry assays. Emphysema was observed histologically at 21 days after 0.5 U PPE treatment; tissues from these mice exhibited increased alveolar linear intercept and air-space volume density in comparison with the control group. TNF-α was elevated at 7 and 14 days after 0.5 U PPE treatment, concomitant with a reduction in the IL-10 levels at the same time points. Myeloperoxidase was elevated in all groups treated with 0.5 U PPE. Oxidative stress was observed during early stages of emphysema, with increased nitrite levels and malondialdehyde and superoxide dismutase activity at 7 days after 0.5 U PPE treatment. Glutathione peroxidase activity was increased in all groups treated with 0.5 U PPE. The emphysema was attenuated when iNOS was inhibited using 1% AMG and in iNOS knockout mice. Furthermore, proteolytic stimulation by PPE enhanced the expression of nitrotyrosine and iNOS, whereas the PPE+AMG group showed low expression of iNOS and nitrotyrosine. PPE stimulus also induced endothelial (e) NOS expression, whereas AMG reduced eNOS. Our results suggest that the oxidative and nitrosative stress pathways are triggered by nitric oxide production via iNOS expression in pulmonary emphysema.

Authors+Show Affiliations

Programa de Pós-graduação em Biologia Humana e Experimental, Rio de Janeiro, RJ, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23000243

Citation

Lanzetti, Manuella, et al. "Oxidative Stress and Nitrosative Stress Are Involved in Different Stages of Proteolytic Pulmonary Emphysema." Free Radical Biology & Medicine, vol. 53, no. 11, 2012, pp. 1993-2001.
Lanzetti M, da Costa CA, Nesi RT, et al. Oxidative stress and nitrosative stress are involved in different stages of proteolytic pulmonary emphysema. Free Radic Biol Med. 2012;53(11):1993-2001.
Lanzetti, M., da Costa, C. A., Nesi, R. T., Barroso, M. V., Martins, V., Victoni, T., Lagente, V., Pires, K. M., e Silva, P. M., Resende, A. C., Porto, L. C., Benjamim, C. F., & Valença, S. S. (2012). Oxidative stress and nitrosative stress are involved in different stages of proteolytic pulmonary emphysema. Free Radical Biology & Medicine, 53(11), 1993-2001. https://doi.org/10.1016/j.freeradbiomed.2012.09.015
Lanzetti M, et al. Oxidative Stress and Nitrosative Stress Are Involved in Different Stages of Proteolytic Pulmonary Emphysema. Free Radic Biol Med. 2012 Dec 1;53(11):1993-2001. PubMed PMID: 23000243.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidative stress and nitrosative stress are involved in different stages of proteolytic pulmonary emphysema. AU - Lanzetti,Manuella, AU - da Costa,Cristiane Aguiar, AU - Nesi,Renata Tiscoski, AU - Barroso,Marina Valente, AU - Martins,Vanessa, AU - Victoni,Tatiana, AU - Lagente,Vincent, AU - Pires,Karla Maria Pereira, AU - e Silva,Patrícia Machado Rodrigues, AU - Resende,Angela Castro, AU - Porto,Luis Cristóvão, AU - Benjamim,Cláudia Farias, AU - Valença,Samuel Santos, Y1 - 2012/09/19/ PY - 2012/04/04/received PY - 2012/04/21/revised PY - 2012/09/13/accepted PY - 2012/9/25/entrez PY - 2012/9/25/pubmed PY - 2013/5/15/medline SP - 1993 EP - 2001 JF - Free radical biology & medicine JO - Free Radic Biol Med VL - 53 IS - 11 N2 - Our aim was to investigate the role of oxidative stress in elastase-induced pulmonary emphysema. C57BL/6 mice were subjected to pancreatic porcine elastase (PPE) instillation (0.05 or 0.5 U per mouse, i.t.) to induce pulmonary emphysema. Lungs were collected on days 7, 14, and 21 after PPE instillation. The control group was sham injected. Also, mice treated with 1% aminoguanidine (AMG) and inducible NO synthase (iNOS) knockout mice received 0.5 U PPE (i.t.), and lungs were analyzed 21 days after. We performed bronchoalveolar lavage, biochemical analyses of oxidative stress, and lung stereology and morphometry assays. Emphysema was observed histologically at 21 days after 0.5 U PPE treatment; tissues from these mice exhibited increased alveolar linear intercept and air-space volume density in comparison with the control group. TNF-α was elevated at 7 and 14 days after 0.5 U PPE treatment, concomitant with a reduction in the IL-10 levels at the same time points. Myeloperoxidase was elevated in all groups treated with 0.5 U PPE. Oxidative stress was observed during early stages of emphysema, with increased nitrite levels and malondialdehyde and superoxide dismutase activity at 7 days after 0.5 U PPE treatment. Glutathione peroxidase activity was increased in all groups treated with 0.5 U PPE. The emphysema was attenuated when iNOS was inhibited using 1% AMG and in iNOS knockout mice. Furthermore, proteolytic stimulation by PPE enhanced the expression of nitrotyrosine and iNOS, whereas the PPE+AMG group showed low expression of iNOS and nitrotyrosine. PPE stimulus also induced endothelial (e) NOS expression, whereas AMG reduced eNOS. Our results suggest that the oxidative and nitrosative stress pathways are triggered by nitric oxide production via iNOS expression in pulmonary emphysema. SN - 1873-4596 UR - https://www.unboundmedicine.com/medline/citation/23000243/Oxidative_stress_and_nitrosative_stress_are_involved_in_different_stages_of_proteolytic_pulmonary_emphysema_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-5849(12)01140-9 DB - PRIME DP - Unbound Medicine ER -