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Molecular characterization and expression of a novel big defensin (Sb-BDef1) from ark shell, Scapharca broughtonii.
Fish Shellfish Immunol 2012; 33(5):1167-73FS

Abstract

Big defensins, endogenous cysteine-rich antimicrobial peptides (AMPs) with antimicrobial activity and immunomodulatory property, play crucial roles in host defense against various microbial pathogens. A novel big defensin (Sb-BDef1) of ark shell Scapharca broughtonii was identified by expressed sequence tag (EST) and RACE techniques. The Sb-BDef1 cDNA contained an open reading frame (ORF) of 336-bp encoding a polypeptide of 111 amino acids with a putative signal peptide of 21 amino acid residues, followed by a putative propeptide of 11 residues and a putative mature peptide of 79 residues. The mature peptide shared the common features of big defensins, including a high hydrophobic residues region (59%) in the N-terminus, a defensin domain in the C-terminus, which perfectly corresponds to the six conserved disulfide-bonded cysteine residues involved in the formation of the internal disulfide bridges (C1-C5, C2-C4 and C3-C6) in all big defensins from mollusk, horseshoe crab and amphioxus. Quantitative real-time PCR analysis revealed that the expression of Sb-BDef1 transcript was detected in all the tissues examined from normal ark shells, and the temporal expression of Sb-BDef1 mRNA was remarkably up-regulated at 8, 16 h in hemocytes, and at 16, 24 h in hepatopancreas after Vibrio anguillarum-challenge, respectively. These results suggested that Sb-BDef1 was a constitutive and inducible acute-phase protein and should be involved in immune response of Gram-negative microbial infection in ark shell S. broughtonii.

Authors+Show Affiliations

Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23000749

Citation

Li, Meng, et al. "Molecular Characterization and Expression of a Novel Big Defensin (Sb-BDef1) From Ark Shell, Scapharca Broughtonii." Fish & Shellfish Immunology, vol. 33, no. 5, 2012, pp. 1167-73.
Li M, Zhu L, Zhou CY, et al. Molecular characterization and expression of a novel big defensin (Sb-BDef1) from ark shell, Scapharca broughtonii. Fish Shellfish Immunol. 2012;33(5):1167-73.
Li, M., Zhu, L., Zhou, C. Y., Sun, S., Fan, Y. J., & Zhuang, Z. M. (2012). Molecular characterization and expression of a novel big defensin (Sb-BDef1) from ark shell, Scapharca broughtonii. Fish & Shellfish Immunology, 33(5), pp. 1167-73. doi:10.1016/j.fsi.2012.09.008.
Li M, et al. Molecular Characterization and Expression of a Novel Big Defensin (Sb-BDef1) From Ark Shell, Scapharca Broughtonii. Fish Shellfish Immunol. 2012;33(5):1167-73. PubMed PMID: 23000749.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization and expression of a novel big defensin (Sb-BDef1) from ark shell, Scapharca broughtonii. AU - Li,Meng, AU - Zhu,Ling, AU - Zhou,Chun-ya, AU - Sun,Shan, AU - Fan,Yan-jun, AU - Zhuang,Zhi-meng, Y1 - 2012/09/18/ PY - 2012/05/25/received PY - 2012/08/22/revised PY - 2012/09/04/accepted PY - 2012/9/25/entrez PY - 2012/9/25/pubmed PY - 2013/3/15/medline SP - 1167 EP - 73 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol. VL - 33 IS - 5 N2 - Big defensins, endogenous cysteine-rich antimicrobial peptides (AMPs) with antimicrobial activity and immunomodulatory property, play crucial roles in host defense against various microbial pathogens. A novel big defensin (Sb-BDef1) of ark shell Scapharca broughtonii was identified by expressed sequence tag (EST) and RACE techniques. The Sb-BDef1 cDNA contained an open reading frame (ORF) of 336-bp encoding a polypeptide of 111 amino acids with a putative signal peptide of 21 amino acid residues, followed by a putative propeptide of 11 residues and a putative mature peptide of 79 residues. The mature peptide shared the common features of big defensins, including a high hydrophobic residues region (59%) in the N-terminus, a defensin domain in the C-terminus, which perfectly corresponds to the six conserved disulfide-bonded cysteine residues involved in the formation of the internal disulfide bridges (C1-C5, C2-C4 and C3-C6) in all big defensins from mollusk, horseshoe crab and amphioxus. Quantitative real-time PCR analysis revealed that the expression of Sb-BDef1 transcript was detected in all the tissues examined from normal ark shells, and the temporal expression of Sb-BDef1 mRNA was remarkably up-regulated at 8, 16 h in hemocytes, and at 16, 24 h in hepatopancreas after Vibrio anguillarum-challenge, respectively. These results suggested that Sb-BDef1 was a constitutive and inducible acute-phase protein and should be involved in immune response of Gram-negative microbial infection in ark shell S. broughtonii. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/23000749/Molecular_characterization_and_expression_of_a_novel_big_defensin__Sb_BDef1__from_ark_shell_Scapharca_broughtonii_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(12)00322-1 DB - PRIME DP - Unbound Medicine ER -