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Complex invasion pattern of the cerebral cortex bymicroglial cells during development of the mouse embryo.
Glia 2013; 61(2):150-63GLIA

Abstract

Microglia are the immune cells of the central nervous system. They are suspected to play important roles in adult synaptogenesis and in the development of the neuronal network. Microglial cells originate from progenitors in the yolk sac. Although it was suggested that they invade the cortex at early developmental stages in the embryo, their invasion pattern remains largely unknown. To address this issue we analyzed the pattern of cortical invasion by microglial cells in mouse embryos at the onset of neuronal cell migration using in vivo immunohistochemistry and ex vivo time-lapse analysis of microglial cells. Microglial cells begin to invade the cortex at 11.5 days of embryonic age (E11.5). They first accumulate at the pial surface and within the lateral ventricles, after which they spread throughout the cortical wall, avoiding the cortical plate region in later embryonic ages. The invasion of the cortical parenchyma occurs in different phases. First, there is a gradual increase of microglial cells between E10.5 and E14.5. From E14.5 to E15.5 there is a rapid phase with a massive increase in microglia, followed by a slow phase again from E15.5 until E17.5. At early stages, many peripheral microglia are actively proliferating before entering the parenchyma. Remarkably, activated microglia accumulate in the choroid plexus primordium, where they are in the proximity of dying cells. Time-lapse analysis shows that embryonic microglia are highly dynamic cells.

Authors+Show Affiliations

Hasselt University, BIOMED, Agoralaan (Gebouw C), Diepenbeek B-3590, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23001583

Citation

Swinnen, Nina, et al. "Complex Invasion Pattern of the Cerebral Cortex Bymicroglial Cells During Development of the Mouse Embryo." Glia, vol. 61, no. 2, 2013, pp. 150-63.
Swinnen N, Smolders S, Avila A, et al. Complex invasion pattern of the cerebral cortex bymicroglial cells during development of the mouse embryo. Glia. 2013;61(2):150-63.
Swinnen, N., Smolders, S., Avila, A., Notelaers, K., Paesen, R., Ameloot, M., ... Rigo, J. M. (2013). Complex invasion pattern of the cerebral cortex bymicroglial cells during development of the mouse embryo. Glia, 61(2), pp. 150-63. doi:10.1002/glia.22421.
Swinnen N, et al. Complex Invasion Pattern of the Cerebral Cortex Bymicroglial Cells During Development of the Mouse Embryo. Glia. 2013;61(2):150-63. PubMed PMID: 23001583.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Complex invasion pattern of the cerebral cortex bymicroglial cells during development of the mouse embryo. AU - Swinnen,Nina, AU - Smolders,Sophie, AU - Avila,Ariel, AU - Notelaers,Kristof, AU - Paesen,Rik, AU - Ameloot,Marcel, AU - Brône,Bert, AU - Legendre,Pascal, AU - Rigo,Jean-Michel, Y1 - 2012/09/21/ PY - 2012/06/15/received PY - 2012/08/28/accepted PY - 2012/9/25/entrez PY - 2012/9/25/pubmed PY - 2013/6/1/medline SP - 150 EP - 63 JF - Glia JO - Glia VL - 61 IS - 2 N2 - Microglia are the immune cells of the central nervous system. They are suspected to play important roles in adult synaptogenesis and in the development of the neuronal network. Microglial cells originate from progenitors in the yolk sac. Although it was suggested that they invade the cortex at early developmental stages in the embryo, their invasion pattern remains largely unknown. To address this issue we analyzed the pattern of cortical invasion by microglial cells in mouse embryos at the onset of neuronal cell migration using in vivo immunohistochemistry and ex vivo time-lapse analysis of microglial cells. Microglial cells begin to invade the cortex at 11.5 days of embryonic age (E11.5). They first accumulate at the pial surface and within the lateral ventricles, after which they spread throughout the cortical wall, avoiding the cortical plate region in later embryonic ages. The invasion of the cortical parenchyma occurs in different phases. First, there is a gradual increase of microglial cells between E10.5 and E14.5. From E14.5 to E15.5 there is a rapid phase with a massive increase in microglia, followed by a slow phase again from E15.5 until E17.5. At early stages, many peripheral microglia are actively proliferating before entering the parenchyma. Remarkably, activated microglia accumulate in the choroid plexus primordium, where they are in the proximity of dying cells. Time-lapse analysis shows that embryonic microglia are highly dynamic cells. SN - 1098-1136 UR - https://www.unboundmedicine.com/medline/citation/23001583/Complex_invasion_pattern_of_the_cerebral_cortex_bymicroglial_cells_during_development_of_the_mouse_embryo_ L2 - https://doi.org/10.1002/glia.22421 DB - PRIME DP - Unbound Medicine ER -