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Ionic liquid-based microwave-assisted extraction for the determination of flavonoid glycosides in pigeon pea leaves by high-performance liquid chromatography-diode array detector with pentafluorophenyl column.
J Sep Sci. 2012 Nov; 35(21):2875-83.JS

Abstract

In this study, an ionic liquid-based microwave-assisted extraction (ILMAE) followed by high-performance liquid chromatography-diode array detector with a pentafluorophenyl column for the extraction and quantification of eight flavonoid glycosides in pigeon pea leaves is described. Compared with conventional extraction methods, ILMAE is a more effective and environment friendly method for the extraction of nature compounds from herbal plants. Nine different types of ionic liquids with different cations and anions were investigated. The results suggested that varying the anion and cation had significant effects on the extraction of flavonoid glycosides, and 1.0 M 1-butyl-3-methylimidazolium bromide ([C4MIM]Br) solution was selected as solvent. In addition, the extraction procedures were also optimized using a series of single-factor experiments. The optimum parameters were obtained as follows: extraction temperature 60°C, liquid-solid ratio 20:1 mL/g and extraction time 13 min. Moreover, an HPLC method using pentafluorophenyl column was established and validated. Good linearity was observed with the regression coefficients (r(2)) more than 0.999. The limit of detection (LODs) (S/N = 3) and limit of quantification (LOQs) (S/N = 10) for the components were less than 0.41 and 1.47 μg/mL, respectively. The inter- and intraday precisions that were used to evaluate the reproducibility and relative standard deviation (RSD) values were less than 4.57%. The recoveries were between 97.26 and 102.69%. The method was successfully used for the analysis of samples of pigeon pea leaves. In conclusion, the developed ILMAE-HPLC-diode array detector using pentafluorophenyl column method can be applied for quality control of pigeon pea leaves and related medicinal products.

Authors+Show Affiliations

Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23001940

Citation

Wei, Wei, et al. "Ionic Liquid-based Microwave-assisted Extraction for the Determination of Flavonoid Glycosides in Pigeon Pea Leaves By High-performance Liquid Chromatography-diode Array Detector With Pentafluorophenyl Column." Journal of Separation Science, vol. 35, no. 21, 2012, pp. 2875-83.
Wei W, Fu YJ, Zu YG, et al. Ionic liquid-based microwave-assisted extraction for the determination of flavonoid glycosides in pigeon pea leaves by high-performance liquid chromatography-diode array detector with pentafluorophenyl column. J Sep Sci. 2012;35(21):2875-83.
Wei, W., Fu, Y. J., Zu, Y. G., Wang, W., Luo, M., Zhao, C. J., Li, C. Y., Zhang, L., & Wei, Z. F. (2012). Ionic liquid-based microwave-assisted extraction for the determination of flavonoid glycosides in pigeon pea leaves by high-performance liquid chromatography-diode array detector with pentafluorophenyl column. Journal of Separation Science, 35(21), 2875-83. https://doi.org/10.1002/jssc.201200473
Wei W, et al. Ionic Liquid-based Microwave-assisted Extraction for the Determination of Flavonoid Glycosides in Pigeon Pea Leaves By High-performance Liquid Chromatography-diode Array Detector With Pentafluorophenyl Column. J Sep Sci. 2012;35(21):2875-83. PubMed PMID: 23001940.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ionic liquid-based microwave-assisted extraction for the determination of flavonoid glycosides in pigeon pea leaves by high-performance liquid chromatography-diode array detector with pentafluorophenyl column. AU - Wei,Wei, AU - Fu,Yu-jie, AU - Zu,Yuan-gang, AU - Wang,Wei, AU - Luo,Meng, AU - Zhao,Chun-jian, AU - Li,Chun-ying, AU - Zhang,Lin, AU - Wei,Zuo-fu, Y1 - 2012/09/24/ PY - 2012/05/16/received PY - 2012/06/22/revised PY - 2012/07/03/accepted PY - 2012/9/25/entrez PY - 2012/9/25/pubmed PY - 2013/3/19/medline SP - 2875 EP - 83 JF - Journal of separation science JO - J Sep Sci VL - 35 IS - 21 N2 - In this study, an ionic liquid-based microwave-assisted extraction (ILMAE) followed by high-performance liquid chromatography-diode array detector with a pentafluorophenyl column for the extraction and quantification of eight flavonoid glycosides in pigeon pea leaves is described. Compared with conventional extraction methods, ILMAE is a more effective and environment friendly method for the extraction of nature compounds from herbal plants. Nine different types of ionic liquids with different cations and anions were investigated. The results suggested that varying the anion and cation had significant effects on the extraction of flavonoid glycosides, and 1.0 M 1-butyl-3-methylimidazolium bromide ([C4MIM]Br) solution was selected as solvent. In addition, the extraction procedures were also optimized using a series of single-factor experiments. The optimum parameters were obtained as follows: extraction temperature 60°C, liquid-solid ratio 20:1 mL/g and extraction time 13 min. Moreover, an HPLC method using pentafluorophenyl column was established and validated. Good linearity was observed with the regression coefficients (r(2)) more than 0.999. The limit of detection (LODs) (S/N = 3) and limit of quantification (LOQs) (S/N = 10) for the components were less than 0.41 and 1.47 μg/mL, respectively. The inter- and intraday precisions that were used to evaluate the reproducibility and relative standard deviation (RSD) values were less than 4.57%. The recoveries were between 97.26 and 102.69%. The method was successfully used for the analysis of samples of pigeon pea leaves. In conclusion, the developed ILMAE-HPLC-diode array detector using pentafluorophenyl column method can be applied for quality control of pigeon pea leaves and related medicinal products. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/23001940/Ionic_liquid_based_microwave_assisted_extraction_for_the_determination_of_flavonoid_glycosides_in_pigeon_pea_leaves_by_high_performance_liquid_chromatography_diode_array_detector_with_pentafluorophenyl_column_ L2 - https://doi.org/10.1002/jssc.201200473 DB - PRIME DP - Unbound Medicine ER -