Tags

Type your tag names separated by a space and hit enter

Investigation of developmental toxicity and teratogenicity of antiemetics on rat embryos cultured in vitro.
Anat Histol Embryol. 2013 Aug; 42(4):239-46.AH

Abstract

In this study, we aimed to investigate and compare the direct toxic and teratogenic effects of dimenhydrinate, metoclopramide and trimethobenzamide HCl, antiemetic drugs on embryonic growth and development in cultured rat embryos. Embryos were explanted on day 9.5 of gestation and cultured. Whole rat serum was used as a culture medium for the control group while different concentrations of dimenhydrinate (2.5-20 μg/ml), metoclopramide (10-50 μg/ml) and trimethobenzamide HCl (25-100 μg/ml) were added to serum for the experimental groups. Effects of antiemetics on embryonic developmental parameters were compared, and embryos were evaluated for the presence of any malformations. Also, the total DNA was extracted from the cells to determine the fragmentation of nuclear DNA of embryonic cells. Compared with the control embryos, the antiemetics significantly decreased all growth and developmental parameters dose dependently. There was no difference regarding the fragmentation of nuclear DNA of the all used agents and controls. Amongst the agents, trimethobenzamide HCl was found to have more toxic and teratogenic potential, and metoclopramide appears to be the least toxic antiemetic and therefore could be more safely used and might be preferred for the treatment of nausea and vomiting in pregnancy.

Authors+Show Affiliations

Department of Anatomy, Selcuklu Medical Faculty, Selcuk University, Konya, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23002952

Citation

Fazliogullari, Z, et al. "Investigation of Developmental Toxicity and Teratogenicity of Antiemetics On Rat Embryos Cultured in Vitro." Anatomia, Histologia, Embryologia, vol. 42, no. 4, 2013, pp. 239-46.
Fazliogullari Z, Karabulut AK, Uysal II, et al. Investigation of developmental toxicity and teratogenicity of antiemetics on rat embryos cultured in vitro. Anat Histol Embryol. 2013;42(4):239-46.
Fazliogullari, Z., Karabulut, A. K., Uysal, I. I., Unver Dogan, N., & Acar, H. (2013). Investigation of developmental toxicity and teratogenicity of antiemetics on rat embryos cultured in vitro. Anatomia, Histologia, Embryologia, 42(4), 239-46. https://doi.org/10.1111/ahe.12007
Fazliogullari Z, et al. Investigation of Developmental Toxicity and Teratogenicity of Antiemetics On Rat Embryos Cultured in Vitro. Anat Histol Embryol. 2013;42(4):239-46. PubMed PMID: 23002952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigation of developmental toxicity and teratogenicity of antiemetics on rat embryos cultured in vitro. AU - Fazliogullari,Z, AU - Karabulut,A K, AU - Uysal,I I, AU - Unver Dogan,N, AU - Acar,H, Y1 - 2012/09/25/ PY - 2012/03/15/received PY - 2012/08/15/accepted PY - 2012/9/26/entrez PY - 2012/9/26/pubmed PY - 2014/1/1/medline SP - 239 EP - 46 JF - Anatomia, histologia, embryologia JO - Anat Histol Embryol VL - 42 IS - 4 N2 - In this study, we aimed to investigate and compare the direct toxic and teratogenic effects of dimenhydrinate, metoclopramide and trimethobenzamide HCl, antiemetic drugs on embryonic growth and development in cultured rat embryos. Embryos were explanted on day 9.5 of gestation and cultured. Whole rat serum was used as a culture medium for the control group while different concentrations of dimenhydrinate (2.5-20 μg/ml), metoclopramide (10-50 μg/ml) and trimethobenzamide HCl (25-100 μg/ml) were added to serum for the experimental groups. Effects of antiemetics on embryonic developmental parameters were compared, and embryos were evaluated for the presence of any malformations. Also, the total DNA was extracted from the cells to determine the fragmentation of nuclear DNA of embryonic cells. Compared with the control embryos, the antiemetics significantly decreased all growth and developmental parameters dose dependently. There was no difference regarding the fragmentation of nuclear DNA of the all used agents and controls. Amongst the agents, trimethobenzamide HCl was found to have more toxic and teratogenic potential, and metoclopramide appears to be the least toxic antiemetic and therefore could be more safely used and might be preferred for the treatment of nausea and vomiting in pregnancy. SN - 1439-0264 UR - https://www.unboundmedicine.com/medline/citation/23002952/Investigation_of_developmental_toxicity_and_teratogenicity_of_antiemetics_on_rat_embryos_cultured_in_vitro_ L2 - https://doi.org/10.1111/ahe.12007 DB - PRIME DP - Unbound Medicine ER -