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[Alleviated affect of exogenous CaCl2 on the growth, antioxidative enzyme activities and cadmium absorption efficiency of Wedelia trilobata hairy roots under cadmium stress].
Sheng Wu Gong Cheng Xue Bao. 2012 Jun; 28(6):747-62.SW

Abstract

In order to study the physiological mechanism of exogenous calcium on the toxicity of heavy metal cadmium (Cd) to Wedelia trilobata hairy roots, the effects of Cd alone, and in combination with different concentrations of Ca on growth, contents of soluble protein and malondialdehyde (MDA), activities of superoxide dismutase (SOD) and peroxidase (POD), Cd2+ absorption in W. trilobata hairy roots were investigated. Cd concentrations lower than 50 micromol/L enhanced the growth of hairy roots, while concentrations higher than 100 micromol/L inhibited growth, making the branched roots short and small, and also turning the root tips brown, even black. In comparison with the control (0 micromol/L Cd), the soluble protein content in hairy roots was found to increase when cultured with 10-50 micromol/L Cd, and decrease when exposed to a cadmium concentration higher than 100 micromol/L Cd. In addition, the activities of POD and SOD activity and MDA content were significantly higher than the control. Compared to the control (hairy roots cultured without 10-30 mmol/L Ca), 100 micromol/L Cd or 300 micromol/L Cd in combination with 10-30 mmol/L Ca resulted in increased growth, causing the main root and secondary roots thicker and also an increase in soluble protein content. On the contrary, MDA content and POD and SOD activities decreased. Quantitative analysis by Atomic Absorption Spectrophotometry showed that W. trilobata hairy roots can absorb and adsorb heavy metal Cd in the ionic form of Cd2+. The maximum content of Cd2+ absorbed by the hairy roots was obtained with a concentration 100 micromol/L Cd2+ while that of Cd2+ adsorbed by hairy roots was achieved with a concentration of 300 micromol/L Cd2+. The exogenous addition of 10-30 mmol/L Ca2+ was found to reduce the absorption, adsorption of Cd2+ and the toxicity of Cd significantly. This reduction in toxicity was caused by the reduction in the absorption of Cd and decreasing the lipid peroxidation through regulating the activities of antioxidant enzymes SOD and POD in the hairy roots.

Authors+Show Affiliations

Shi H
Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, Guangdong, China. shihp@scnu.edu.cn
Wang Y
No affiliation info available
Tsang PE
No affiliation info available
Chan LA
No affiliation info available

MeSH

AbsorptionAdsorptionCadmiumCalcium ChloridePeroxidasePlant RootsStress, PhysiologicalSuperoxide DismutaseWedelia

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23016311

Citation

Shi, Heping, et al. "[Alleviated Affect of Exogenous CaCl2 On the Growth, Antioxidative Enzyme Activities and Cadmium Absorption Efficiency of Wedelia Trilobata Hairy Roots Under Cadmium Stress]." Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, vol. 28, no. 6, 2012, pp. 747-62.
Shi H, Wang Y, Tsang PE, et al. [Alleviated affect of exogenous CaCl2 on the growth, antioxidative enzyme activities and cadmium absorption efficiency of Wedelia trilobata hairy roots under cadmium stress]. Sheng Wu Gong Cheng Xue Bao. 2012;28(6):747-62.
Shi, H., Wang, Y., Tsang, P. E., & Chan, L. A. (2012). [Alleviated affect of exogenous CaCl2 on the growth, antioxidative enzyme activities and cadmium absorption efficiency of Wedelia trilobata hairy roots under cadmium stress]. Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, 28(6), 747-62.
Shi H, et al. [Alleviated Affect of Exogenous CaCl2 On the Growth, Antioxidative Enzyme Activities and Cadmium Absorption Efficiency of Wedelia Trilobata Hairy Roots Under Cadmium Stress]. Sheng Wu Gong Cheng Xue Bao. 2012;28(6):747-62. PubMed PMID: 23016311.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Alleviated affect of exogenous CaCl2 on the growth, antioxidative enzyme activities and cadmium absorption efficiency of Wedelia trilobata hairy roots under cadmium stress]. AU - Shi,Heping, AU - Wang,Yunling, AU - Tsang,PoKeung Eric, AU - Chan,LeeWah Andrew, PY - 2012/9/29/entrez PY - 2012/9/29/pubmed PY - 2013/9/27/medline SP - 747 EP - 62 JF - Sheng wu gong cheng xue bao = Chinese journal of biotechnology JO - Sheng Wu Gong Cheng Xue Bao VL - 28 IS - 6 N2 - In order to study the physiological mechanism of exogenous calcium on the toxicity of heavy metal cadmium (Cd) to Wedelia trilobata hairy roots, the effects of Cd alone, and in combination with different concentrations of Ca on growth, contents of soluble protein and malondialdehyde (MDA), activities of superoxide dismutase (SOD) and peroxidase (POD), Cd2+ absorption in W. trilobata hairy roots were investigated. Cd concentrations lower than 50 micromol/L enhanced the growth of hairy roots, while concentrations higher than 100 micromol/L inhibited growth, making the branched roots short and small, and also turning the root tips brown, even black. In comparison with the control (0 micromol/L Cd), the soluble protein content in hairy roots was found to increase when cultured with 10-50 micromol/L Cd, and decrease when exposed to a cadmium concentration higher than 100 micromol/L Cd. In addition, the activities of POD and SOD activity and MDA content were significantly higher than the control. Compared to the control (hairy roots cultured without 10-30 mmol/L Ca), 100 micromol/L Cd or 300 micromol/L Cd in combination with 10-30 mmol/L Ca resulted in increased growth, causing the main root and secondary roots thicker and also an increase in soluble protein content. On the contrary, MDA content and POD and SOD activities decreased. Quantitative analysis by Atomic Absorption Spectrophotometry showed that W. trilobata hairy roots can absorb and adsorb heavy metal Cd in the ionic form of Cd2+. The maximum content of Cd2+ absorbed by the hairy roots was obtained with a concentration 100 micromol/L Cd2+ while that of Cd2+ adsorbed by hairy roots was achieved with a concentration of 300 micromol/L Cd2+. The exogenous addition of 10-30 mmol/L Ca2+ was found to reduce the absorption, adsorption of Cd2+ and the toxicity of Cd significantly. This reduction in toxicity was caused by the reduction in the absorption of Cd and decreasing the lipid peroxidation through regulating the activities of antioxidant enzymes SOD and POD in the hairy roots. SN - 1000-3061 UR - https://www.unboundmedicine.com/medline/citation/23016311/[Alleviated_affect_of_exogenous_CaCl2_on_the_growth_antioxidative_enzyme_activities_and_cadmium_absorption_efficiency_of_Wedelia_trilobata_hairy_roots_under_cadmium_stress]_ L2 - https://antibodies.cancer.gov/detail/CPTC-PTPRC-1 DB - PRIME DP - Unbound Medicine ER -