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Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection.
J Virol Methods. 2013 Jan; 187(1):114-20.JV

Abstract

Dengue virus (DENV) is the most prevalent arbovirus in the world, found mainly in tropical regions. As clinical manifestations present frequently as nonspecific febrile illness, laboratory diagnosis is essential to confirm DENV infections and for epidemiological studies. Recombinant envelope (E) antigens of four serotypes of DENV were used to develop an immunoglobulin G enzyme-linked immunosorbent assay (IgG-ELISA). To evaluate the IgG-ELISA, a panel of serum samples that had been tested previously by a plaque reduction neutralization test (PRNT) was investigated for the presence of anti-E antibodies against the four DENV serotypes. IgG-ELISA was found to have a sensitivity (91%) and specificity (98%) at a receiver-operating characteristic (ROC) optimized cutoff and demonstrated high performance as well as good indexes. A concordance of 97% was achieved between both assays, and only 21/704 (3%) samples were not concordant. The results of the present study demonstrate a moderate correlation between neutralizing antibody titers and IgG-ELISA values. These findings indicate that the recombinant protein-based IgG-ELISA is a suitable method for routine serodiagnosis, monitoring and seroepidemiological studies of DENV infections.

Authors+Show Affiliations

Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Laboratório de Vírus, Belo Horizonte, MG, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23018061

Citation

Rocha, Eliseu Soares de Oliveira, et al. "Recombinant Envelope Protein-based Enzyme Immunoassay for IgG Antibodies Is Comparable to Neutralization Tests for Epidemiological Studies of Dengue Infection." Journal of Virological Methods, vol. 187, no. 1, 2013, pp. 114-20.
Rocha ES, Oliveira JG, Santos JR, et al. Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection. J Virol Methods. 2013;187(1):114-20.
Rocha, E. S., Oliveira, J. G., Santos, J. R., Rodrigues, G. O., Figueiredo, L. B., Pessanha, J. E., Proietti, F. A., Fonseca, F. G., Bonjardim, C. A., Ferreira, P. C., & Kroon, E. G. (2013). Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection. Journal of Virological Methods, 187(1), 114-20. https://doi.org/10.1016/j.jviromet.2012.09.012
Rocha ES, et al. Recombinant Envelope Protein-based Enzyme Immunoassay for IgG Antibodies Is Comparable to Neutralization Tests for Epidemiological Studies of Dengue Infection. J Virol Methods. 2013;187(1):114-20. PubMed PMID: 23018061.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection. AU - Rocha,Eliseu Soares de Oliveira, AU - Oliveira,Jaquelline Germano de, AU - Santos,João Rodrigues dos, AU - Rodrigues,Gisele Olinto Libânio, AU - Figueiredo,Leandra Barcelos, AU - Pessanha,José Eduardo Marques, AU - Proietti,Fernando Augusto, AU - Fonseca,Flávio Guimarães da, AU - Bonjardim,Claudio Antonio, AU - Ferreira,Paulo Cesar Peregrino, AU - Kroon,Erna Geessien, Y1 - 2012/09/24/ PY - 2012/01/18/received PY - 2012/09/12/revised PY - 2012/09/13/accepted PY - 2012/9/29/entrez PY - 2012/9/29/pubmed PY - 2013/6/21/medline SP - 114 EP - 20 JF - Journal of virological methods JO - J Virol Methods VL - 187 IS - 1 N2 - Dengue virus (DENV) is the most prevalent arbovirus in the world, found mainly in tropical regions. As clinical manifestations present frequently as nonspecific febrile illness, laboratory diagnosis is essential to confirm DENV infections and for epidemiological studies. Recombinant envelope (E) antigens of four serotypes of DENV were used to develop an immunoglobulin G enzyme-linked immunosorbent assay (IgG-ELISA). To evaluate the IgG-ELISA, a panel of serum samples that had been tested previously by a plaque reduction neutralization test (PRNT) was investigated for the presence of anti-E antibodies against the four DENV serotypes. IgG-ELISA was found to have a sensitivity (91%) and specificity (98%) at a receiver-operating characteristic (ROC) optimized cutoff and demonstrated high performance as well as good indexes. A concordance of 97% was achieved between both assays, and only 21/704 (3%) samples were not concordant. The results of the present study demonstrate a moderate correlation between neutralizing antibody titers and IgG-ELISA values. These findings indicate that the recombinant protein-based IgG-ELISA is a suitable method for routine serodiagnosis, monitoring and seroepidemiological studies of DENV infections. SN - 1879-0984 UR - https://www.unboundmedicine.com/medline/citation/23018061/Recombinant_envelope_protein_based_enzyme_immunoassay_for_IgG_antibodies_is_comparable_to_neutralization_tests_for_epidemiological_studies_of_dengue_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-0934(12)00326-6 DB - PRIME DP - Unbound Medicine ER -