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Occurrence and characterization of multidrug-resistant New Delhi metallo-β-lactamase-1-producing bacteria isolated between 2003 and 2010 in Bangladesh.
J Med Microbiol. 2013 Jan; 62(Pt 1):62-8.JM

Abstract

The purpose of this study was to screen for reduced susceptibility against imipenem and the presence of the New Delhi metallo-β-lactamase-1 (NDM-1) gene in a collection of Enterobacteriaceae (Escherichia coli, Shigella spp. and Klebsiella pneumoniae) from different surveillance studies between 2003 and 2010 at the International Centre for Diarrhoeal Disease Research, Bangladesh. None of the E. coli (n = 1789) and Shigella spp. (n = 90) isolated between 2009 and 2010 from stool samples was resistant or had intermediate susceptibility to imipenem. Among 127 extended-spectrum β-lactamase-producing strains isolated during 2003-2009, three Klebsiella pneumoniae isolates (2.4 %) were resistant to imipenem and were positive for bla(NDM-1). All these NDM-1-producing strains were isolated in 2008 and were resistant to all antibiotics tested except for tigecycline and colistin. All three isolates were positive for bla(OXA-1) group, bla(CTX-M-1) group (bla(CTX-M-15)) and bla(SHV) genes, whilst two isolates were positive for 16S rRNA methylase (armA) and qnr (qnrB) genes. One isolate was positive for the bla(CMY) gene and one for the rmtB gene. The bla(NDM-1) gene was located on a conjugative plasmid of ~23-24 MDa. The PFGE patterns of the isolates were different from each other. This study highlights the occurrence of NDM-1-producing organisms in Bangladesh in 2008. The clonal diversity of the isolates and the transferability of bla(NDM-1) plasmids suggest a wider distribution of NDM-1-producing bacteria in Bangladesh.

Authors+Show Affiliations

Center for Food and Waterborne Diseases, International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh. maislam@icddrb.orgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23019191

Citation

Islam, Mohammad Aminul, et al. "Occurrence and Characterization of Multidrug-resistant New Delhi Metallo-β-lactamase-1-producing Bacteria Isolated Between 2003 and 2010 in Bangladesh." Journal of Medical Microbiology, vol. 62, no. Pt 1, 2013, pp. 62-8.
Islam MA, Huq M, Nabi A, et al. Occurrence and characterization of multidrug-resistant New Delhi metallo-β-lactamase-1-producing bacteria isolated between 2003 and 2010 in Bangladesh. J Med Microbiol. 2013;62(Pt 1):62-8.
Islam, M. A., Huq, M., Nabi, A., Talukdar, P. K., Ahmed, D., Talukder, K. A., Cravioto, A., & Endtz, H. P. (2013). Occurrence and characterization of multidrug-resistant New Delhi metallo-β-lactamase-1-producing bacteria isolated between 2003 and 2010 in Bangladesh. Journal of Medical Microbiology, 62(Pt 1), 62-8. https://doi.org/10.1099/jmm.0.048066-0
Islam MA, et al. Occurrence and Characterization of Multidrug-resistant New Delhi Metallo-β-lactamase-1-producing Bacteria Isolated Between 2003 and 2010 in Bangladesh. J Med Microbiol. 2013;62(Pt 1):62-8. PubMed PMID: 23019191.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Occurrence and characterization of multidrug-resistant New Delhi metallo-β-lactamase-1-producing bacteria isolated between 2003 and 2010 in Bangladesh. AU - Islam,Mohammad Aminul, AU - Huq,Mohsina, AU - Nabi,Ashikun, AU - Talukdar,Prabhat Kumar, AU - Ahmed,Dilruba, AU - Talukder,Kaisar Ali, AU - Cravioto,Alejandro, AU - Endtz,Hubert P, Y1 - 2012/09/27/ PY - 2012/9/29/entrez PY - 2012/9/29/pubmed PY - 2013/2/19/medline SP - 62 EP - 8 JF - Journal of medical microbiology JO - J. Med. Microbiol. VL - 62 IS - Pt 1 N2 - The purpose of this study was to screen for reduced susceptibility against imipenem and the presence of the New Delhi metallo-β-lactamase-1 (NDM-1) gene in a collection of Enterobacteriaceae (Escherichia coli, Shigella spp. and Klebsiella pneumoniae) from different surveillance studies between 2003 and 2010 at the International Centre for Diarrhoeal Disease Research, Bangladesh. None of the E. coli (n = 1789) and Shigella spp. (n = 90) isolated between 2009 and 2010 from stool samples was resistant or had intermediate susceptibility to imipenem. Among 127 extended-spectrum β-lactamase-producing strains isolated during 2003-2009, three Klebsiella pneumoniae isolates (2.4 %) were resistant to imipenem and were positive for bla(NDM-1). All these NDM-1-producing strains were isolated in 2008 and were resistant to all antibiotics tested except for tigecycline and colistin. All three isolates were positive for bla(OXA-1) group, bla(CTX-M-1) group (bla(CTX-M-15)) and bla(SHV) genes, whilst two isolates were positive for 16S rRNA methylase (armA) and qnr (qnrB) genes. One isolate was positive for the bla(CMY) gene and one for the rmtB gene. The bla(NDM-1) gene was located on a conjugative plasmid of ~23-24 MDa. The PFGE patterns of the isolates were different from each other. This study highlights the occurrence of NDM-1-producing organisms in Bangladesh in 2008. The clonal diversity of the isolates and the transferability of bla(NDM-1) plasmids suggest a wider distribution of NDM-1-producing bacteria in Bangladesh. SN - 1473-5644 UR - https://www.unboundmedicine.com/medline/citation/23019191/Occurrence_and_characterization_of_multidrug_resistant_New_Delhi_metallo_β_lactamase_1_producing_bacteria_isolated_between_2003_and_2010_in_Bangladesh_ L2 - http://jmm.microbiologyresearch.org/pubmed/content/journal/jmm/10.1099/jmm.0.048066-0 DB - PRIME DP - Unbound Medicine ER -